Anti p erk 4377s

Fudosteine (Fud, purity ≥98%) was purchased from Feiyu Biological Technology (Nantong, Jiangsu, China). SU11274 (a selective HGF antagonist, purity ≥98%) was purchased from ApexBio (Houston, TX, USA). LPS was purchased from Sigma-Aldrich (St. Louis, MO, USA). TNF-α was purchased from Peprotech (Rocky Hill, USA). Anti-HGF (BS6234) was purchased from Bioworld (St. Paul, MN, USA). Anti-p-ERK (#4377S) and the HRP-coupled secondary antibody (#7074S or #7076S) were purchased from Cell Signal Technology (Beverly, MA, USA). Anti-MUC5AC (sc-21701) and anti-EGFR (sc-80543) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-CD11b (101206) was purchased from Biolegend (San Diego, CA, USA). Anti-ERK (51068-1-AP) and anti-GAPDH (HRP-60004) were purchased from Proteintech (Chicago, IL, USA). HGF, MUC5AC, IL-6 and IL-13 Enzyme-linked Immunosorbent Assay (ELISA) kits were purchased from Elabscience Biotechnology (Wuhan, Hubei, China). Phenol red was purchased from Maikun Chemical (Shanghai, China). Rotring ink was obtained from Rotring (Hamburg, Germany).

Cells transfected with plasmids or RNA transcripts were washed, fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X‐100 and subsequently blocked. Samples were incubated with primary antibody, anti‐p‐ERK (4377s; Cell Signaling Technology), or anti‐VP1 (MAB979; Merck Millipore) at a dilution of 1:200, then with secondary antibodies (1:500), and finally with DAPI for 5 min. Cells were examined by confocal microscopy (Leica) using Leica fluorescence microscope (Leica DFC 365 FX).