cDNA was prepared using the Quanta qScript mRNA cDNA Synthesis Kit (Quantabio, Beverly, MA, USA) according to the manufacturer’s instructions and diluted 1:10 in double-distilled water prior to qPCR plate preparation. qPCR was performed in either 384-well or 96-well plates, using Perfecta Sybr Green FastMix with low or No Rox (Quantabio) at a final well volume of 5 or 15 μl, respectively. As housekeeping genes, tubulin-β3 class III was used for human brain samples and for SH-SY5Y cells, and glyceraldehyde 3-phosphate dehydrogenase was used for HEK-293T cells. Expression was calculated as ΔΔCt values (primer sequences are detailed in Supplemental Table S2 ).
Perfecta sybr green fastmix with low or no rox
Manufactured by Quantabio
Perfecta Sybr Green FastMix with low or No Rox is a ready-to-use qPCR master mix designed for high-performance real-time PCR applications. It contains a proprietary buffer system, thermostable DNA polymerase, and SYBR Green I dye for sensitive detection of double-stranded DNA products.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using perfecta sybr green fastmix with low or no rox
1
Quantitative Gene Expression Analysis
2
Quantitative PCR Analysis of Gene Expression
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cDNA was prepared using the Quanta qScript mRNA cDNA Synthesis Kit (Quantabio, Beverly, MA, USA) according to the manufacturer’s instructions and diluted 1:10 in double-distilled water prior to qPCR plate preparation. qPCR was performed in either 384-well or 96-well plates on CFX-384/96 machines (Bio-Rad), using Perfecta Sybr Green FastMix with low or No Rox (Quantabio) at a final well volume of 5 or 15 μL, respectively. β-actin and RPL-19 were used as housekeeping genes. Expression was calculated as ΔΔCt values using the Bio-Rad CFX Maestro 1.1 Software Version 4.1.2433.1219.
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