Human quantikine immunoassay kits

Blood was harvested into serum tubes to determine levels of interferon-γ (INF-γ), interferon gamma–induced protein-10 (IP-10), monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1 β), interleukin-17 (IL-17), and interleukin-10 (IL-10). Collection tubes were centrifuged over 10 min at 4 °C at 1000 g. Serum was then transferred into cryotubes and stored at – 80 °C until analysis. Serum levels of IFN-γ, IP-10, and MCP-1 were evaluated by Human Quantikine® Immunoassay kits (R&D Systems, MN, USA), sensitivities: 1.28 pg/ml, 4.46 pg/ml, and 10 pg/ml, respectively. Circulating levels of TNF-α, IL-6, IL-1 β, IL-17, and IL-10 were assessed using Quantikine® High Sensitivity Human Immunoassay kits (R&D Systems, MN, USA) with sensitivities: 0.049 pg/ml, 0.09 pg/ml, 0.063 pg/ml, 0.051 pg/ml, and 0.17 pg/ml, respectively. All assays were performed according to directions provided by the kits’ manufacturer. Within-assay coefficient of variation of all analyses was less than 10%.