Jc 1 kit

Zinc acetate and 2-methylimidazole (2-MeIM) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Glucose oxidase (GOx), ethanol, and 4,4-azodianiline were purchased from Aladdin Co., Ltd. (Shanghai, China). Dimethyl sulfoxide (DMSO), and mesitylene (trimethyl benzene, TMB) were purchased from Sigma–Aldrich (Shanghai, China) Trading Co., Ltd. Phosphate-buffered saline (PBS, 10×), and Dulbecco's modified Eagle's medium (DMEM) were purchased from KeyGEN (Nanjing, China). Fetal bovine serum (FBS) was purchased from ExCell Bio (Taicang, China). Penicillin/streptomycin (P/S, 100 ×), Bovine albumin (BSA), and DAPI stain solution were purchased from Sangon Biotech (Shanghai, China) Co., Ltd. Cell Counting Kit-8 (CCK-8) was purchased from TargetMol (Boston, MA, USA). JC-1 kit, propidium iodide (PI), and calcein-AM dyes, Annexin V-FITC/PI Apoptosis Detection Kit (Cat No. 40302ES50) were acquired from Yeasen Biotechnology (Shanghai, China). 2 × Phanta Max Master Mix, ChamQ SYBR® qPCR Master Mix (High ROX Premixed), and FastPure Cell/Tissue DNA Isolation Mini Kit were purchased from Vazyme Biotech Co., Ltd. (Nanjing, China). 0.05% trypsin solution was purchased from YIFEIXUE BIOTECH (Nanjing, China). RIPA buffer and Phenylmethylsulfonyl fluoride (PMSF) were purchased from Solarbio (Beijing, China).