Doxorubicin hcl dox

N-(3-dimethylaminopropyl)-N’-ethyl-carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), 3-(aminopropyl)triethoxysilane (APTES), ethanol (absolute, ≥99.8%), doxorubicin HCl (DOX), and recombinant human serum albumin (rHSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline (PBS) was purchased from Gibco (Grand Island, NY, USA). Live Cell ExplorerTM live-cell labeling kit was purchased from AAT Bioquest^® (Sunnyvale, CA, USA). CellTiter-GlO^® Luminescent Cell Viability Assay was purchased from Promega (Madison, WI, USA).

MCF7, MDA-MB-231, and SKBR3 breast carcinoma cells, HeLa cervical adenocarcinoma cells, U-2OS osteosarcoma cells are from American Type Culture Collection (ATCC, Manassas, VA). ZR75-1 breast carcinoma cells are a gift from Dr. Natalia Marchenko (Stony Brook University). RPMI, DMEM, F12/DMEM, and McCoy’s 5A culture medium, fetal bovine serum (FBS), and superscript III reverse transcriptase (RT) are from Life Technologies (Carlsbad, CA). Bio-Rad protein assay was from Bio-Rad (Hercules, CA). Antibodies for total PARP, total Src, phospho-Src (Y416), Yes, p53, ATM, ATR, E-cadherin, n-cadherin, and Vimentin were from Cell Signaling Technology (Danvers, MA). Anti-actin antibody, doxorubicin-HCl (Dox), and Dasatinib were from Sigma (St. Louis, MO). HRP-labeled secondary antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). Chemiluminescence kit was from Thermo Scientific (Suwanee, GA). Fluorescence-labeled antibodies for CD44 and CD24 were from BD Biosciences (San Jose, CA). Rhodamine phalloidin was from Invitrogen (Carlsbad, CA). Fluoroshield mounting media with DAPI was from Abcam (Cambridge, UK).

Avanti Polar Lipids provided methoxy‐polyethylene glycol (MW 2000)–distearoylphosphatidylethanoloamine (mPEG 2000–DSPE) (Alabaster, AL). Maleimide PEG2000 di stearoyl phosphatidylethanolamine or DSPE‐PEG (2000) Maleimide was purchased from Avantipolar (Alabaster, AL). Furthermore, Nanocs Inc. provided 1,2‐Distearoyl‐sn‐glycero‐3‐phosphoethanolamine‐N‐ [succinimidyl (polyethylene glycol)] (DSPE–PEG3400 NHS) (USA). Merck supplied the ‐L‐Glutamyl‐L‐cysteinyl‐glycine (L‐Glutathione). Behestan Darou and Sigma‐Aldrich provided Caelyx^® and Doxorubicin HCl (DOX) respectively. Merck provided the isopropanol, which was acidified by adding 7.5 ml HCl 1M and 2.5 ml water to 90 ml isopropanol. The chemical grade was applied to all other reagents and solvents.