Tr213

Manufactured by Randox

Sourced in United Kingdom

The TR213 is a clinical chemistry analyzer designed for in-vitro diagnostic testing. It provides automated analysis of a variety of biochemical parameters in biological samples. The TR213 utilizes spectrophotometric techniques to deliver accurate and reliable results.

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Lab products found in correlation

As521, by Randox (2 mentions) Al520, by Randox (2 mentions) Ch201, by Randox (2 mentions) Ab230932, by Abcam (1 mentions) Sb 1010 225, by Thermo Fisher Scientific (1 mentions) Mpc900, by R&D Systems (1 mentions)

5 protocols using tr213

Plasma and Tissue Lipid Quantification

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Ten-microliter plasma samples were directly treated with 1 mL of working reagent (TR213, Randox), absorbance read at 500 nm after 5-min incubation at 37 °C. Hepatic tissue samples were first extracted with a proper amount of solvent (chloroform: methanol 2: 1, v/v) and Triton x-100 added. Extracts were vacuumed and reconstituted with the working reagent (TR213, Randox). Subsequent procedures were the same as those for serum samples [15 (link)].

Chiu W.C., Yang H.H., Chiang S.C., Chou Y.X, & Yang H.T. (2014). Auricularia polytricha aqueous extract supplementation decreases hepatic lipid accumulation and improves antioxidative status in animal model of nonalcoholic fatty liver. BioMedicine, 4(2), 12.

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Hepatic Triglyceride Quantification

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Total triglyceride (TG) were extracted from liver tissues using Folch solution (chloroform-methanol, 2:1 v/v), dried and dissolved in 100% EtOH^{47 (link)}. Hepatic lipid extracts were assayed for TG levels using commercial assay kits (Randox, TR213).

Kim Y.H., Lee J.H., Yeung J.L., Das E., Kim R.Y., Jiang Y., Moon J.H., Jeong H., Thakkar N., Son J.E., Trzaskalski N., Hui C.C., Doh K.O., Mulvihill E.E., Kim J.R., Kim K.H, & Sung H.K. (2019). Thermogenesis-independent metabolic benefits conferred by isocaloric intermittent fasting in ob/ob mice. Scientific Reports, 9, 2479.

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Serum Biomarker Measurement Protocol

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Blood serum was obtained by centrifuging at 1000 × g for 10 min. Total triglycerides (TR213, Randox Laboratories, Antrim, UK), total cholesterol (CH201, Randox Laboratories, Antrim, UK), aspartate aminotransferase (AST) (AS521, Randox Laboratories, Antrim, UK), alanine aminotransferase (ALT) (AL520, Randox Laboratories, Antrim, UK), gamma-glutamyl transferase (γGT) (GT523, Randox Laboratories, Antrim, UK), and alpha-fetoprotein (AFP) (AB360, Randox Laboratories, Antrim, UK), were determined using by enzymatic colorimetric methods using commercial kits (Randox Laboratories, Antrim, UK), respectively [27 (link)].

Yang M.Y., Hung T.W., Wang C.J, & Tseng T.H. (2019). Inhibitory Effect of Nelumbo nucifera Leaf Extract on 2-Acetylaminofluorene-induced Hepatocarcinogenesis Through Enhancing Antioxidative Potential and Alleviating Inflammation in Rats. Antioxidants, 8(9), 329.

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Biochemical Markers Profiling of Metabolic Status

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Plasma levels of aspartate aminotransferase (AST; AS521, Randox Laboratories, Antrim, UK), alanine aminotransferase (ALT; AL520, Randox Laboratories), total cholesterol (CH201, Randox Laboratories), and triglycerides (TR213, Randox Laboratories) were determined through enzymatic colorimetric methods using commercial kits (Randox Laboratories). In addition, levels of insulin (Mercodia AB, Uppsala, Sweden) and glycosylated hemoglobin (HbA1c; Fortress Diagnostics, UK) were determined.

Lee A.T., Yang M.Y., Lee Y.J., Yang T.W., Wang C.C, & Wang C.J. (2021). Gallic Acid Improves Diabetic Steatosis by Downregulating MicroRNA-34a-5p through Targeting NFE2L2 Expression in High-Fat Diet-Fed db/db Mice. Antioxidants, 11(1), 92.

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Multiparametric Serum Lipoprotein Analysis

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Sera were analyzed by a colorimetric assay for total cholesterol (SB-1010–225, Fisher Scientific, Hampton NH) and by ELISAs for PCSK9 (MPC900, R&D Systems, Minneapolis MN) and APOB (ab230932, abcam, Cambridge UK). Serum lipoprotein fractionation assays were performed at the University of Cincinnati Mouse Metabolic Phenotyping Center. Sera were pooled from 5 mice for each genotype and fractionated by fast liquid protein chromatography (FPLC) into 50 fractions. Cholesterol (NC9343696, Fisher, Hampton NH) and triglyceride (TR213, Randox Laboratories, Crumlin UK) content in each fraction were determined using a microliter plate enzyme-based assay. Liver function tests were performed at the University of Michigan In-Vivo Animal Core (IVAC) with sera collected from individual mice using a Liasys analyzer (AMS Alliance).

Tang V.T., McCormick J., Xu B., Wang Y., Fang H., Wang X., Siemieniak D., Khoriaty R., Emmer B.T., Chen X.W, & Ginsburg D. (2022). Hepatic inactivation of murine Surf4 results in marked reduction in plasma cholesterol. eLife, 11, e82269.

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