Ab168338

Manufactured by Abcam

Ab168338 is a laboratory product that serves as a specific antibody for research purposes. The core function of this product is to provide researchers with a tool for identifying and studying a particular target analyte.

Automatically generated - may contain errors

Lab products found in correlation

Ab124822, by Abcam (3 mentions) Ab168381, by Abcam (2 mentions) C myc sc 40, by Santa Cruz Biotechnology (2 mentions) Gfp sc 9996, by Santa Cruz Biotechnology (2 mentions) α synuclein ab27766, by Abcam (2 mentions) α synuclein ab138501, by Abcam (2 mentions) α synuclein sc 12767, by Santa Cruz Biotechnology (2 mentions) Enolase sc 15343, by Santa Cruz Biotechnology (2 mentions) Hsp60 sc 13115, by Santa Cruz Biotechnology (2 mentions) Th mab318, by Merck Group (2 mentions) Clpp gtx115070, by GeneTex (2 mentions) Clpp nbp1 89557, by Novus Biologicals (2 mentions) Eral1 11478 1 ap, by Proteintech (2 mentions) Chchd3 25624 1 ap, by Proteintech (2 mentions) Sod2 611580, by BD (2 mentions) Mfn1 h00055669 m04, by Abnova (2 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) β actin a1978, by Merck Group (1 mentions) Wfs1 11558 1 ap, by Proteintech (1 mentions) Ab227387, by Abcam (1 mentions)

4 protocols using ab168338

Protein Phosphatase and Protease Inhibitor Cocktails

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Protein phosphatase inhibitor and protease inhibitor cocktails were purchased from Sigma-Aldrich. Antibodies for ClpX (ab168338, 1:2000), ClpP (ab124822, 1:1000), VDAC (ab34726, 1:1000), α-Synuclein (ab27766, 1:1000), α-Synuclein (ab138501, 1:3000) and α-Synuclein phosphor S129 (ab168381, 1:1000) were from Abcam. GFP (sc-9996, 1:1000), c-Myc (sc-40, 1:1000), α-Synuclein (sc-12767, 1:1000), Enolase (sc-15343, 1:1000) and HSP60 (sc-13115, 1:2000) were from Santa Cruz Biotechnology. β-Actin (A1978, 1:10000) was from Sigma-Aldrich. TH (MAB318, 1:1000) was from Millipore. ClpP (GTX115070, 1:200) was from Genetex. ClpP (NBP1–89557, 1:200) was from Novus. LonP (15440–1-AP, 1:2000), ERAL1 (11478–1-AP, 1:2000), CHCHD3 (25624–1-AP, 1:1000) and WFS1 (11558–1-AP, 1:1000) were from Proteintech. SOD2 (611580, 1:1000) was from BD bioscience. MFN1 (H00055669-M04, 1:1000) was from Abnova. Sirt3 (5490S, 1:1000) was from cell signaling technology.

Hu D., Sun X., Liao X., Zhang X., Zarabi S., Schimmer A., Hong Y., Ford C., Luo Y, & Qi X. (2019). Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity. Acta Neuropathologica, 137(6), 939-960.

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Antibody panel for mitochondrial proteome

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Protein phosphatase inhibitor and protease inhibitor cocktails were purchased from Sigma–Aldrich. Antibodies for ClpX (ab168338, 1:2000), ClpP (ab124822, 1:1000), VDAC (ab34726, 1:1000), α-Synuclein (ab27766, 1:1000), α-Synuclein (ab138501, 1:3000) and α-Synuclein phosphor S129 (ab168381, 1:1000) were from Abcam. GFP (sc-9996, 1:1000), c-Myc (sc-40, 1:1000), α-Synuclein (sc-12767, 1:1000), Enolase (sc-15343, 1:1000) and HSP60 (sc-13115, 1:2000) were from Santa Cruz Biotechnology. β-Actin (A1978, 1:10000) was from Sigma–Aldrich. TH (MAB318, 1:1000) was from Millipore. ClpP (GTX115070, 1:200) was from Genetex. ClpP (NBP1-89557, 1:200) was from Novus. LonP (15440-1-AP, 1:2000), ERAL1 (11478-1-AP, 1:2000), CHCHD3 (25624-1-AP, 1:1000) and WFS1 (11558-1-AP, 1:1000) were from Proteintech. SOD2 (611580, 1:1000) was from BD bioscience. MFN1 (H00055669-M04, 1:1000) was from Abnova. Sirt3 (5490S, 1:1000) was from cell signaling technology.

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Protein Extraction and Western Blot Analysis

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Mouse testes were homogenized on ice and lysed in lysis buffer (50 mmol l⁻¹ Tris-HCl pH 8.0, 120 mmol l⁻¹ NaCl, 1 mmol l⁻¹ EDTA, 6 mmol l⁻¹ ethylene glycol-bis[β-aminoethyl ether]-N,N,N´,N´-tetraacetic acid [EGTA], 1% Nonidet P-40, 1 mmol l⁻¹ dithiothreitol, 10 mmol l⁻¹ NaF, 0.25 mmol l⁻¹ Na₃VO₄, and 50 mmol l⁻¹ β-glycerophosphate) supplemented with complete protease inhibitors (4693159001, Roche, Basel, Switzerland). The lysate was centrifuged at 20 000g (5804R, Eppendorf, Hamburg, Germany) for 30 min at 4°C, and the supernatant was used for western blotting. The following primary antibodies were used: rabbit anti-ClpX (1:3000, ab168338, Abcam), mouse anti-total OXPHOS (1:1000, ab110413, Abcam), and rabbit anti-β-actin (1:5000, #ab227387, Abcam). The secondary antibodies used were goat anti-mouse IgG-horseradish peroxidase (HRP)-conjugated (1:5000, FDM007, Fdbio Science, Hangzhou, China) and goat anti-rabbit IgG-HRP-conjugated (1:5000, FDR007, Fdbio Science). Relative protein levels, obtained after normalization to β-actin, were used to standardize the loading variations.

Feng H.W., Zhao Y., Gao Y.L., Liu D.T, & Huo L.J. (2023). Caseinolytic mitochondrial matrix peptidase X is essential for homologous chromosome synapsis and recombination during meiosis of male mouse germ cells. Asian Journal of Andrology, 26(2), 165-174.

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Carnosine Treatment Alters Mitochondrial Proteins

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The cells were treated with carnosine for 48 hours and then were lysed in Western
and IP lysis buffer containing PMSF for 5 minutes on ice, followed by
centrifugation at 13 000 × g for 25 minutes at 4°C. The
supernatant was harvested, and the protein concentration was quantified using a
BCA protein assay kit. Western blot analysis was carried out by standard
protocol. The following antibodies were used: rabbit anti-c-Myc antibody
(1:5000, ab32072), rabbit anti-PCNA antibody (1:1000, ab92552), rabbit
anti-Bcl-2 antibody (1:1000, ab32124), rabbit anti-SDHA antibody (1:1000,
ab137040), rabbit anti-IDH3A antibody (1:1000, ab58641), rabbit anti-MDH1
antibody (1:1000, ab180152), rabbit anti-ClpP antibody (1:1000, ab124822),
rabbit anti-ClpX antibody (1:1000, ab168338), rabbit anti-COX IV antibody
(1:1000, ab66739) (from Abcam Inc). Mouse anti-β-actin antibody (1:1000, AA128),
HRP-labeled goat anti-rabbit IgG (1:500, A0208), and HRP-labeled goat anti-mouse
IgG (1:500, A0216) were from Beyotime Institute of Biotechnology (Nanjing,
China).

Bao Y., Ding S., Cheng J., Liu Y., Wang B., Xu H., Shen Y, & Lyu J. (2016). Carnosine Inhibits the Proliferation of Human Cervical Gland Carcinoma Cells Through Inhibiting Both Mitochondrial Bioenergetics and Glycolysis Pathways and Retarding Cell Cycle Progression. Integrative Cancer Therapies, 17(1), 80-91.

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