Ba0532

Manufactured by Boster Bio

BA0532 is a laboratory equipment product. It serves a core function in scientific research and analysis. The detailed specifications and intended use of this product are not available in this response.

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Lab products found in correlation

Ba0407, by Boster Bio (1 mentions) Pb0245, by Boster Bio (1 mentions) Kit 9706, by Maixin Group (1 mentions) Ar0005, by Boster Bio (1 mentions) Ab28364, by Abcam (1 mentions) α actin, by Merck Group (1 mentions) Fluorescence microscopy, by Olympus (1 mentions) A0262, by ABclonal (1 mentions) Cellsens dimension, by Olympus (1 mentions) Orb10573, by Biorbyt (1 mentions) Ab6538, by Abcam (1 mentions) Ab16667, by Abcam (1 mentions)

4 protocols using ba0532

Immunohistochemical Analysis of Angiogenic Factors

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Paraffin-embedded tissue sections (4 μm) were subjected to antigen retrieval, incubation with anti-CD31 (ab28364, abcam), anti-VEGF-A (BA0407, Boster), anti-HIF-1A (PB0245, Boster), anti-CD34 (BA0532, Boster), or anti-TSP1 (ab93653, abcam) antibodies, followed by incubation with a peroxidase-conjugated goat anti-rabbit IgG (KIT-9706, Maixin-Bio) secondary antibody. Samples were developed with DAB or AEC and counterstained with Mayor's hematoxylin (AR0005, Boster). Image Pro Plus 6.0 software was used for quantitative analysis.

Gao L., Chen K., Gao Q., Wang X., Sun J, & Yang Y.G. (2016). CD47 deficiency in tumor stroma promotes tumor progression by enhancing angiogenesis. Oncotarget, 8(14), 22406-22413.

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In Vivo Cardiac CTRP9 Overexpression

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Green fluorescent protein (GFP)-conjugated CTRP9 lentivirus (LV. CTRP9) or negative control (NC) lentivirus (NC) were injected into the left ventricle of mice (30 μl) and rats (100 μl) (Table 1). Frozen heart sections were prepared after 72 h transfection. Lentiviral location was assessed by staining with α-actin (Sigma-Aldrich, A2547) or CD31 (Boster, BA0532) antibodies (Supplemental Figs. 1B and 2A). Five sections from each heart were examined by fluorescence microscopy (Olympus, Japan). The overexpression efficiency was assessed by Western blot. Plasma CTRP9 was determined by Enzyme-Linked Immunosorbent Assay (Aviscera Bioscience, SK00081-08) to detect whether the lentivirus mediated a cardiac-specific CTRP9 overexpression (Supplemental Fig. 1D).

Zhao D., Feng P., Sun Y., Qin Z., Zhang Z., Tan Y., Gao E., Lau W.B., Ma X., Yang J., Yu S., Xu X., Yi D, & Yi W. (2018). Cardiac-derived CTRP9 protects against myocardial ischemia/reperfusion injury via calreticulin-dependent inhibition of apoptosis. Cell Death & Disease, 9(7), 723.

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Immunohistochemical Analysis of Tumor Mechanisms

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To investigate the mechanisms of action, we performed immunohistochemical studies on tumor tissues from 3 groups, following our previously developed procedures [30 (link)]. The parameters included, tumor cell apoptosis markers Tunel, bcl-2/6 (Maxim- 0598, Bio Co, CHN, working solution), tumor cell proliferation marker Ki-67 (MAB-0129, Maxim-Bio Co, CHN, working solution), tumor angiogenesis CD34 (BA0532, WuHan Boster Bio-Engineering Co, CHN, 1:100), VEGF (RB-9031, Maxim-Bio Co, CHN, working solution), and tumor lymphatic marker D2-40 (AM0103, Ascend Biotechnology Co, CHN, working solution). The results determination and scoring were performed according to the method described by [33 (link)], and presented as the median and range from three animals per group.

Zhang J., Yuan J.P., Wang Q., Shao L.H., Liu S.P., Firestone R.A., Hong Y.P., Li J.G., Xin Y.C, & Li Y. (2017). Experimental evidence of good efficacy and reduced toxicity with peptide-doxorubicin to treat gastric cancer. Oncotarget, 9(2), 1957-1968.

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Immunohistochemical Analysis of HPV-Related Markers

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Paraffin-embedded sections (5 μm) were subjected to H&E staining and immunohistochemical (IHC) staining. The slides were incubated overnight at 4°C with rabbit anti-HPV16 E7 (1:50, orb10573, Biorbyt), rabbit anti-P16 (1:100, A0262, Abclonal), rabbit anti-RB1 (1:100, 10048-2-Ig, Proteintech), rabbit anti-Ki67 (1:100, ab16667, Abcam), rabbit anti-CD34 (1:100, BA0532, Wuhan Boster Bio-Engineering), rabbit anti-CDK2 (1:400, ab6538, Abcam), and rabbit anti-E2F1 (1:200, 12171-1-Ap, Proteintech) as primary antibodies. Antibody detection was performed using diaminobenzidine (DAB). Images were photographed from three randomly chosen fields using cellSens Dimension (version 1.8.1, Olympus). To quantitatively analyze the expression level, the staining score was determined according to the staining depth and the ratio of positive stained positive cells. Dye depth: 0 is no dye, 1 is light yellow, 3 is brown-yellow, 2 is between 1 and 3. The proportion of positive stained positive cells is calculated to the total number of cells. Staining score = staining depth × positive stained cell ratio.

Xiong J., Tan S., Yu L., Shen H., Qu S., Zhang C., Ren C., Zhu D, & Wang H. (2021). E7-Targeted Nanotherapeutics for Key HPV Afflicted Cervical Lesions by Employing CRISPR/Cas9 and Poly (Beta-Amino Ester). International Journal of Nanomedicine, 16, 7609-7622.

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