Deparaffinized and hydrated sections were heated in citrate solution (pH 6.0) for antigen retrieval (DAKO). After blocking for 1 h. using 2% BSA, sections were incubated with primary antibodies that were either from rabbit or mouse to: hEPCAM (Invitrogen 1:200), hACE-2 (Abcam 1:200), Muc5AC (Abcam, 1:200), SARS-CoV-2 Spike (Abcam, 1:100) [9 (link)] and SARS-CoV-2 nucleocapsid (Sinobiogical, 1:100) [10 (link)], overnight. Following further PBS washs, the appropriate Alexa-Fluor-conjugated secondary antibodies (Molecular Probes, Eugene, OR, 1:700) were incubated for 1 h. at room temperature, washed in PBS, mounted in Slow fade for imaging. Immunostained slides were imaged with an FSX100 microscope (Olympus) and exposure-matched pictures from negative controls compared.
Hace 2
Manufactured by Abcam
Sourced in United States
HACE-2 is a recombinant human histone acetyltransferase complex subunit HACE1. It is a protein that plays a role in the acetylation of histones, which can regulate gene expression.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
Fsx100 microscope, by Olympus (1 mentions)
Antigen retrieval, by Agilent Technologies (1 mentions)
Muc5ac, by Abcam (1 mentions)
Sars cov 2 spike, by Abcam (1 mentions)
Flag tag m2, by Merck Group (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Sars cov 2 rbd, by Sino Biological (1 mentions)
Ripa buffer, by Santa Cruz Biotechnology (1 mentions)
Hrp conjugated goat antimouse or rabbit igg, by Southern Biotech (1 mentions)
Gm130, by Abcam (1 mentions)
Calnexin, by Abcam (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
2 protocols using hace 2
1
SARS-CoV-2 Immunohistochemistry Protocol
2
Western Blot Analysis of SARS-CoV-2 RBD and EV Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells, purified EVs or tissues were lysed with RIPA buffer (Santa Cruz,USA) and cleared lysate was collected by centrifugation for protein separation on SDS-polyacrylamide gel(10%), followed by transfer onto PVDF membranes(Millipore), blocked with 5% nonfat dry milk in TBST and reacted with primary antibodies recognizing FLAG M2 tag(Sigma,1:2000), SARS-CoV-2 RBD(Sinobiological,1:500), CD9(Abcam,1:2000), Alix(Cell Signaling Technology,1:1000), GM130(Abcam,1:1000), Calnexin(Abcam,1:1000), hACE2(Abcam,# ab108209,only react with human species,1:2000) and GAPDH(Santa Cruz,1:2000). Blots were incubated with HRP-conjugated goat anti-mouse or rabbit IgG (Southern Biotech,1:5000) for 1 h at room temperature before imaging.
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