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One step taqman rt qpcr

Manufactured by Thermo Fisher Scientific
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The One Step Taqman RT-qPCR is a real-time quantitative reverse transcription PCR (RT-qPCR) assay. It enables the detection and quantification of RNA targets in a single reaction.

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Lab products found in correlation

7500 real time pcr system, by Thermo Fisher Scientific (2 mentions) Rneasy plus mini kit, by Qiagen (1 mentions)

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TaqMan qPCR (43 citations) TaqMan RT-qPCR (5 citations)

2 protocols using one step taqman rt qpcr

1

ZIKV RNA Quantification from Tissues

Cited 1 time
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RNA was extracted from brain, eye and spleen tissue samples using RNeasy Plus Mini Kit (QIAgen), following the manufacturer’s recommendations. Viral RNA was quantified using One Step Taqman RT-qPCR (Thermo Fisher Scientific) on a 7500 Real-Time PCR System (Applied Biosystems) with primers and probe designed by Lanciotti and collaborators44 (link). ZIKV copies were calculated by interpolation onto an internal standard curve formed of eight 10-fold serial dilutions of a synthetic ZIKV RNA based on the Asian lineage. The ZIKV quantification was displayed in ZIKV RNA copies per gram of tissue.
Garcez P.P., Stolp H.B., Sravanam S., Christoff R.R., Ferreira J.C., Dias A.A., Pezzuto P., Higa L.M., Barbeito-Andrés J., Ferreira R.O., Andrade C.B., Siqueira M., Santos T.M., Drumond J., Hoerder-Suabedissen A., de Lima C.V., Tovar-Moll F., Lopes R.T., Fragel-Madeira L., Lent R., Ortiga-Carvalho T.M., Stipursky J., Bellio M., Tanuri A, & Molnár Z. (2018). Zika virus impairs the development of blood vessels in a mouse model of congenital infection. Scientific Reports, 8, 12774.
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2

Quantifying ZIKV Viral Particles' Inhibition

Cited 1 time
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Viral RNA was extracted from 200 μL supernatant of infected cells using QIAmp MiniElute Virus Spin (QIAgen, Hilden, Düsseldorf ,DE), following the manufacturer’s recommendations. ZIKV detection was performed using One Step Taqman RT-qPCR (Thermo Fisher Scientific) on a 7500 Real-Time PCR System (Applied Biosystems) with primers and the probe described elsewhere [2 (link)]. Threshold cycle (CT) was determined and ∆CT (CT chloroquine treated − CT untreated) was calculated. The fold reduction of virus particles’ release, including defective viral particles, were calculated by 2∆Ct.
Delvecchio R., Higa L.M., Pezzuto P., Valadão A.L., Garcez P.P., Monteiro F.L., Loiola E.C., Dias A.A., Silva F.J., Aliota M.T., Caine E.A., Osorio J.E., Bellio M., O’Connor D.H., Rehen S., de Aguiar R.S., Savarino A., Campanati L, & Tanuri A. (2016). Chloroquine, an Endocytosis Blocking Agent, Inhibits Zika Virus Infection in Different Cell Models. Viruses, 8(12), 322.
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