Vet scan analyzer

Blood was collected from sedated animals at scheduled time points, as described in Section 2.2 and Figure 1a. Coagulation analysis, complete blood counts (CBC), and clinical chemistry analyses were performed, as previously described [38 (link),39 (link),40 (link),41 (link)]. Serum was also analyzed for C-Reactive Protein (CRP) levels using a Piccolo BioChemistry Panel Plus on a Vet Scan analyzer (Abaxis, Inc., Union City, CA, USA). Hematology and serum chemistry parameters shown below (Table 1) are provided (Figure 1c–h). The coagulation assessments performed were activated partial thromboplastin time (aPTT) and prothrombin time (PT) (Figure 1i,j).

Biochemical analysis was performed using the mammalian liver enzyme profile rotor on a Vet Scan analyzer (Abaxis, Inc., Union City, CA, USA). Coagulation times were determined using the IDEXX Coag Dx Analyzer (IDEXX Laboratories, Westbrook, ME, USA). Vetscan HM2 hematology analyzer (Abaxis, Inc., Union City, CA, USA) (IACUC 1381) or a Procyte Dx Hematology Analyzer (IDEXX laboratories, Westbrook, ME, USA) (IACUCs 1532 and 1529) was used to obtain neutrophil, lymphocyte, and red blood cell counts.

Blood was collected from the abdominal aorta and collected in 15-mL plain tubes and lavender-top (EDTA) specimen tubes for analysis of hemoglobin A1C (HbA1C). Within 1 h of collection, plain tube blood samples were then centrifuged at 3000 rpm for 10 min to collect serum. The sera were immediately stored at −80 °C to analyze blood urea nitrogen (BUN), serum creatine (sCr), glucose levels, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) activities, by using an Olympus AU400 chemistry analyzer (Tokyo, Japan). Advanced glycation end products (AGEs) using the VetScan analyzer (Abaxis Inc., Union City, CA, USA). The levels of urinary albumin, creatinine, and protein were estimated by using a Hitachi 7180 auto analyzer (Hitachi, Tokyo, Japan).

Whole blood was collected at pre-determined study days 7, 0, 3, 4, 5, 6, at scheduled euthanasia, and at moribund euthanasia. Whole blood was collected into tubes containing Ethylenediaminetetraacetic acid (EDTA) for Complete blood Counts (CBC) analysis and clinical chemistry. CBC was performed using a Procyte Dx Hematology Analyzer (IDEXX laboratories, Westbrook, Maine, USA) and the parameters analyzed included white blood cell counts, neutrophil counts and percent, lymphocytes counts and percent, monocytes counts and percent, red blood cell counts, percent hematocrit, reticulocyte counts and percent, platelet counts, and percent platelet. Clinical chemistry was performed using the mammalian liver enzyme profile rotor on a Vet Scan analyzer (Abaxis, Inc., Union City, CA, USA). Coagulation times were determined on whole blood with no additives using an IDEXX Coag Dx Analyzer (IDEXX Laboratories, Westbrook, Maine, USA). Parameters were evaluated against reference ranges established by Texas Biomed clinical pathology derived from a cohort of clinically healthy macaques.