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Recombinant human interferon gamma protein active ab259377

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Recombinant Human Interferon gamma protein (Active) (ab259377) is a purified recombinant protein produced in a HEK293 cell expression system. The protein is in an active form and has a molecular weight of approximately 17 kDa.

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Lab products found in correlation

Ab50036, by Abcam (2 mentions) Paraformaldehyde solution aaj19943k2, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole dihydrochloride dapi fluoropure grade, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Corning (2 mentions)

2 protocols using recombinant human interferon gamma protein active ab259377

1

Prostate Cancer Cell Culture Protocol

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lines were as previously described [51 (link),52 (link)]. The DU145, 22Rv1 and LNCaP prostate cancer cell lines were cultured in RPMI-1640 medium (Gibco). PC3 cells were cultured in F12K (Kaighn’s) medium (Gibco, 21127022). All culture media were supplemented with 10% fetal bovine serum (FBS, Corning) and 1% antibiotics (penicillin/streptomycin, Gibco). As positive controls for induction of EMT genes or PD-L1, we treated the cells with transforming growth factor (TGF)-β or interferon (IFN)-γ (5 ng/mL), respectively, as we previously published [53 (link)]. Paraformaldehyde solution (AAJ19943K2, Thermo Scientific) and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; FluoroPure grade; D21490, Thermo Scientific) were used to fix and stain the nuclei. Recombinant Human Interferon gamma protein (Active) (ab259377), and Recombinant human TGF beta 1 protein (ab50036) were purchased from Abcam.
Jafari N., Chen A., Kolla M., Pompa I.R., Qiu Y., Yu R., Llevenes P., Ennis C.S., Mori J., Mahdaviani K., Halpin M., Gignac G.A., Heaphy C.M., Monti S, & Denis G.V. (2022). Novel plasma exosome biomarkers for prostate cancer progression in co-morbid metabolic disease. Advances in cancer biology - metastasis, 6, 100073.
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2

Prostate Cancer Cell Culture Protocol

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lines were as previously described [51 (link),52 (link)]. The DU145, 22Rv1 and LNCaP prostate cancer cell lines were cultured in RPMI-1640 medium (Gibco). PC3 cells were cultured in F12K (Kaighn’s) medium (Gibco, 21127022). All culture media were supplemented with 10% fetal bovine serum (FBS, Corning) and 1% antibiotics (penicillin/streptomycin, Gibco). As positive controls for induction of EMT genes or PD-L1, we treated the cells with transforming growth factor (TGF)-β or interferon (IFN)-γ (5 ng/mL), respectively, as we previously published [53 (link)]. Paraformaldehyde solution (AAJ19943K2, Thermo Scientific) and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; FluoroPure grade; D21490, Thermo Scientific) were used to fix and stain the nuclei. Recombinant Human Interferon gamma protein (Active) (ab259377), and Recombinant human TGF beta 1 protein (ab50036) were purchased from Abcam.
Jafari N., Chen A., Kolla M., Pompa I.R., Qiu Y., Yu R., Llevenes P., Ennis C.S., Mori J., Mahdaviani K., Halpin M., Gignac G.A., Heaphy C.M., Monti S, & Denis G.V. (2022). Novel plasma exosome biomarkers for prostate cancer progression in co-morbid metabolic disease. Advances in cancer biology - metastasis, 6, 100073.
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