Anti osx

Manufactured by Santa Cruz Biotechnology

Sourced in Germany, Japan

Anti-Osx is an antibody product developed by Santa Cruz Biotechnology. It is designed to detect the Osterix (Osx) protein, which is a transcription factor involved in osteoblast differentiation and bone formation.

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4 protocols using anti osx

Immunohistochemical Analysis of Dlx3 and Osx in Molar Development

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The expressions of Dlx3 and Osx in mandibular first molars of both wild type and Bmp2-cKO^od mice were analyzed by immunohistochemistry as described previously^{57 (link)}. MD10-F2 cells cultured on glass slides were fixed with cold methanol/acetone (1:1) for 10 minutes and permeabilized with 0.5% Triton-X for 15 minutes. To block the non-specific binding of antibodies, slides were incubated with 10% goat serum for 30 minutes, followed by primary antibodies, anti-Dlx3 (Abcam) and anti-Osx (Santa Cruz), overnight at 4^oC. After washing with PBS, slides were incubated with secondary IgG antibodies conjugated to Alexa-Fluor 568 (Invitrogen) were added and incubated for 1 hour. Hoechst (Pierce) was used to stain nucleus.

Yang G., Yuan G., MacDougall M., Zhi C, & Chen S. (2017). BMP-2 induced Dspp transcription is mediated by Dlx3/Osx signaling pathway in odontoblasts. Scientific Reports, 7, 10775.

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Osteogenic Differentiation Markers Quantification

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Osterix and osteopontin bone-specific markers were assessed by confocal microscopy after 7 and 28 days of osteogenic differentiation. Constructs were fixed with 4% PFA for 8 h and permeabilized with 2% BSA/0.1% Triton X-100 solution at 4 °C. Next, samples were incubated 4 h at 37 °C with mouse polyclonal anti-Osx and goat polyclonal anti-Opn (Santa-Cruz Biotechnology, Heidelberg, Germany) antibodies. The bioconstructs were further incubated in tetramethylrodamine-5,6-isothiocyanate (TRITC) conjugated goat anti mouse and fluorescein isothiocyanate (FITC) conjugated rabbit anti goat secondary antibodies solutions for 1 h (Santa-Cruz Biotechnology, Heidelberg, Germany). Nuclei were stained with DAPI for 30 min. Finally, samples were visualized in confocal microscopy.

Dinescu S., Ionita M., Ignat S.R., Costache M, & Hermenean A. (2019). Graphene Oxide Enhances Chitosan-Based 3D Scaffold Properties for Bone Tissue Engineering. International Journal of Molecular Sciences, 20(20), 5077.

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Western Blot Analysis of Osteogenic Markers

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Western blot analysis was performed as in our previous report [63 (link)]. Briefly, the primary (anti-OCN, anti-OSX, anti-integrin α5, anti-integrin β1, anti-BMPR I, anti-BMPR II, anti-SMAD1/5/8, anti-P-SMAD1/5/8, anti-ERK1/2, anti-P-ERK1/2, anti-p38, anti-P-p38, anti-JNK, anti-P-JNK, or anti-β-actin; Santa Cruz Biotechnology) and secondary antibodies (goat anti-rabbit immunoglobulin G (IgG) or goat anti-mouse IgG conjugated to horseradish peroxidase) were employed with the dilutions recommended by the supplier. The blots were developed using enhanced chemiluminescence (Santa Cruz Biotechnology) and developed using X-ray film (Eastman-Kodak, Rochester, NY, USA).

Shim N.Y, & Heo J.S. (2021). Performance of the Polydopamine-Graphene Oxide Composite Substrate in the Osteogenic Differentiation of Mouse Embryonic Stem Cells. International Journal of Molecular Sciences, 22(14), 7323.

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Western Blot Analysis of Osteogenic Markers

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Proteins (30 μg) were dissolved in sample buffer, and electrophoresis was carried out at a current of 25 mA for 2 h. Proteins were transferred from SDS-PAGE onto nitrocellulose membranes (Schleicher & Schuell, Dassel, Germany). Membranes were blocked for 1 h with 5% nonfat dry milk in PBS containing 0.1% Tween-20 (PBS-T) and incubated overnight with anti-Osx (Santa Cruz Biotechnology), TβRII (Santa Cruz Biotechnology), Runx2 (Abcam), DMP1 (TaKaRa Bio, Shiga, Japan), Bsp (Abcam), Opn (Abcam), Cyclin D1 (Santa Cruz Biotechnology) and β-Actin (Santa Cruz Biotechnology) IgG diluted in PBS-T buffer at 4 °C. After washing, the membranes were incubated with anti-rabbit or mouse-IgG conjugated horseradish peroxidase (Santa Cruz Biotechnology) for 1 h. Labeled protein bands were detected using an enhanced chemiluminescence system (Amersham Biosciences, Buckinghamshire, UK). Protein expression levels were analyzed with the ImageQuanT TL 1D gel analysis program (Amersham Biosciences).

Choi H., Ahn Y.H., Kim T.H., Bae C.H., Lee J.C., You H.K, & Cho E.S. (2016). TGF-β Signaling Regulates Cementum Formation through Osterix Expression. Scientific Reports, 6, 26046.

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