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Nova view automated fluorescence microscope system

Manufactured by Inova Diagnostics
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The NOVA View is an automated fluorescence microscope system. It is designed to capture and analyze fluorescence-based images.

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Lab products found in correlation

Nova lite hep 2 ana slide with dapi kit, by Inova Diagnostics (4 mentions)

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NOVA View (7 citations)

4 protocols using nova view automated fluorescence microscope system

1

Standardized Indirect Immunofluorescence Assay

Cited 1 time
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Serum samples were evaluated by indirect immunofluorescence at a 1:80 dilution using the NOVA Lite HEp-2 ANA slide with DAPI kit (INOVA Diagnostics, San Diego, CA, USA) and a highly specific fluorescein isothiocyanate conjugated secondary antibody (goat anti-human IgG). Images captured via the NOVA View automated fluorescence microscope system (INOVA Diagnostics) were stored digitally. Immunofluorescence staining intensities were assigned integer grades from 0 to 4, relative to standard references, with nonzero grades indicating ANA positivity (Dinse et al. 2020 (link)). All samples were assayed in a single laboratory, using identical methods. At least two experienced evaluators made independent readings, blinded to participant characteristics and time period, and they agreed on over 95% of the grades; differences were resolved by consensus or adjudicated by a third blinded rater. Repeat testing of 200 random samples showed over 98% concordance.
Dinse G.E., Co C.A., Parks C.G., Weinberg C.R., Xie G., Chan E.K., Birnbaum L.S, & Miller F.W. (2022). Expanded assessment of xenobiotic associations with antinuclear antibodies in the United States, 1988–2012. Environment international, 166, 107376.
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2

Standardized Indirect Immunofluorescence Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum samples were evaluated by indirect immunofluorescence at a 1:80 dilution using the NOVA Lite HEp-2 ANA slide with DAPI kit (INOVA Diagnostics, San Diego, CA, USA) and a highly specific fluorescein isothiocyanate conjugated secondary antibody (goat anti-human IgG). Images captured via the NOVA View automated fluorescence microscope system (INOVA Diagnostics) were stored digitally. Immunofluorescence staining intensities were assigned integer grades from 0 to 4, relative to standard references, with nonzero grades indicating ANA positivity (Dinse et al. 2020 (link)). All samples were assayed in a single laboratory, using identical methods. At least two experienced evaluators made independent readings, blinded to participant characteristics and time period, and they agreed on over 95% of the grades; differences were resolved by consensus or adjudicated by a third blinded rater. Repeat testing of 200 random samples showed over 98% concordance.
Dinse G.E., Co C.A., Parks C.G., Weinberg C.R., Xie G., Chan E.K., Birnbaum L.S, & Miller F.W. (2022). Expanded assessment of xenobiotic associations with antinuclear antibodies in the United States, 1988–2012. Environment international, 166, 107376.
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3

Standardized Indirect Immunofluorescence Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum samples were evaluated by indirect immunofluorescence at a 1:80 dilution using the NOVA Lite HEp-2 ANA slide with DAPI kit (INOVA Diagnostics, San Diego, CA, USA) and a highly specific fluorescein isothiocyanate conjugated secondary antibody (goat anti-human IgG). Images captured via the NOVA View automated fluorescence microscope system (INOVA Diagnostics) were stored digitally. Immunofluorescence staining intensities were assigned integer grades from 0 to 4, relative to standard references, with nonzero grades indicating ANA positivity (Dinse et al. 2020 (link)). All samples were assayed in a single laboratory, using identical methods. At least two experienced evaluators made independent readings, blinded to participant characteristics and time period, and they agreed on over 95% of the grades; differences were resolved by consensus or adjudicated by a third blinded rater. Repeat testing of 200 random samples showed over 98% concordance.
Dinse G.E., Co C.A., Parks C.G., Weinberg C.R., Xie G., Chan E.K., Birnbaum L.S, & Miller F.W. (2022). Expanded assessment of xenobiotic associations with antinuclear antibodies in the United States, 1988–2012. Environment international, 166, 107376.
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4

ANA Immunofluorescence Staining Assay

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum samples were shipped with dry ice and stored at −80°C until evaluated by indirect immunofluorescence at a 1:80 dilution using the NOVA Lite HEp-2 ANA slide with DAPI kit (INOVA Diagnostics), with a highly specific fluorescein isothiocyanate-conjugated secondary antibody (goat anti-human IgG). Images were captured using the NOVA View automated fluorescence microscope system (INOVA Diagnostics) and stored digitally. Immunofluorescence staining intensities were graded using a 0-4 scale compared to standard references (8 (link)). Participants who had grades of 1-4 were positive for ANA; those with grades of 3 or 4 were further assessed by sequential ANA titers up to 1:1280 dilution. ANA patterns, including nuclear, cytoplasmic, or mitotic, were defined according to international consensus (15 (link)). All serum samples were assayed using the same methods in a single laboratory. Readings were made independently by at least 2 experienced evaluators (who were blinded with regard to sample characteristics and time period), who agreed on >95% of the intensities and patterns; differences were resolved by consensus or adjudicated by a third blinded rater (EKLC) who was also blinded with regard to sample characteristics. Repeat testing of random samples showed >98% concordance.
Grevelink S.A., Youssef D.E., Loscalzo J, & Lerner E.A. (1993). Salivary gland extracts from the deerfly contain a potent inhibitor of platelet aggregation.. Proceedings of the National Academy of Sciences of the United States of America, 90(19), 9155-9158.
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