Kms34

The human MM cell lines, KMS-34, were purchased from the Japanese Collection of Research Bioresources (JCRB, Osaka, Japan). KMS-34 cells were cultured in RPMI-1640 supplemented with 10% FBS and 1% penicillin-streptomycin (Nacalai Tesque, Kyoto, Japan). Cell lines were maintained in a humidified incubator at 37°C with a 5% CO₂ atmosphere.
The KMS-34 cells expressing PELI2, ELF3, EREG, and mock-transfected cells were established as follows; cDNAs encoding human PELI2, ELF3, and EREG were separately subcloned into the pMP71CW-IRES-EGFP vectors, a kind gift from Dr. T. Chinen (Kyushu University, Japan). These vectors and empty vector were transfected into KMS-34 cells by lipofection using vesicular stomatitis virus G protein pseudotyped vector particles, respectively.

Fourteen MM cell lines and HS‐5 bone marrow stromal cells (BMSC) were used. EJM (ACC‐560), LP‐1 (ACC‐41) and OPM‐2 (ACC‐50) were purchased from DSMZ. KMS‐11 (JCRB1179) was obtained from the Health Science Research Resources Bank. KMS‐12‐BM (JCRB0429), KMS‐12‐PE (JCRB0430), KMS‐21‐BM (JCRB1185), KMS‐26 (JCRB1187), KMS‐28‐BM (JCRB1192) and KMS‐34 (JCRB1195) were obtained from the Japanese Collection of Research Bioresources Cell Bank. HS‐5 (CRL‐11882), MM.1S (CRL‐2974), MM.1R (CRL‐2975), NCI‐H929 (CRL‐9068) and U266B1 (TIB‐196) were obtained from the American Type Culture Collection. EMJ was cultured in Iscove’s modified Dulbecco’s medium supplemented with 20% FBS. HS‐5 was cultured in DMEM supplemented with 10% FBS. NCI‐H929 was cultured in RPMI 1640 medium supplemented with 10% FBS and 0.05 mmol/L 2‐mercaptoethanol. U266B1 was cultured in RPMI 1640 medium supplemented with 15% FBS. The other cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS. All cell lines used were authenticated by means of short tandem repeat‐based DNA profiling.