A 1 mm square piece of cellulose acetate membrane filter (0.8 μm pore size; Schleicher and Schuell, Keene, NH) was infused with a saturated solution of Texas Red dextran amine (TDA; 3,000 MW; Molecular Probes) in ethanol and left to air-dry so that particles of TDA were embedded within the filter. The optic nerve was exposed and cut close to the back of eyeball. The piece of filter was inserted into the stump, and the palate was sutured. After 48 hours, the frog was anesthetized, fixed and the retina was sectioned for immunocytochemistry as described above.
Texas red dextran amine
Manufactured by Thermo Fisher Scientific
Texas Red dextran amine is a fluorescent dye that can be used as a tracer or labeling agent in various biological applications. It consists of Texas Red, a red-emitting fluorophore, covalently linked to dextran, a polysaccharide. This product provides a stable, water-soluble fluorescent label that can be used to track the movement and distribution of molecules or cells within biological systems.
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Lab products found in correlation
2 protocols using texas red dextran amine
1
Retinal Labeling via Cellulose Acetate Membrane
2
Retrograde Labeling of Retinal Ganglion Cells
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A 1 mm square piece of cellulose acetate membrane filter (0.8 μm pore size; Schleicher and Schuell, Keene, NH) was infused with a saturated solution of Texas Red dextran amine (TDA; 3,000 MW; Molecular Probes) in ethanol and left to air-dry so that particles of TDA were embedded within the filter. The optic nerve of control or experimental animals was exposed and cut close to the back of the eyeball. The piece of filter was inserted into the stump and the palate was sutured. After 48 − 60 hours, the frog was anesthetized, and fixed. With these parameters, TDA consistently labeled 80 − 84% of the cells in the GCL of control retinas. The percentage of displaced amacrine cells in Rana pipiens retina has been previously calculated as 16% [48 (link)], so we appear to be labeling most of the RGCs. The percentage of total cells in the GCL labeled with TDA decreased with time after axotomy; this was not due to a reduced efficacy of the retrograde labeling procedure but corresponded to the expected decline in RGC numbers at these times.
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