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A1r hd25 confocal laser scanning microscope

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The Nikon A1R HD25 is a confocal laser scanning microscope. It is designed to provide high-resolution imaging capabilities for various applications. The core function of the A1R HD25 is to capture detailed, high-quality images through the use of a laser scanning system and advanced optical components.

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Nis elements ar software, by Nikon (1 mentions)

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4 protocols using a1r hd25 confocal laser scanning microscope

1

Confocal Imaging with Nikon A1R HD25

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Confocal images were collected using a Nikon A1R HD25 confocal laser scanning microscope equipped with LU-N4/N4S 4-laser unit.
Zhang S.J., Lowe L.A., Anees P., Krishnan Y., Fai T.G., Szostak J.W, & Wang A. (2023). Passive endocytosis in model protocells. Proceedings of the National Academy of Sciences of the United States of America, 120(24), e2221064120.
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2

Visualizing Peptide Uptake in U87MG Cells

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U87MG cells were seeded in a confocal dish with a cell density at ≈105 and incubated at 37 °C for 24 h. Cell suspensions were removed and washed three times with PBS buffers to remove any non-adherent cell. 20 μL of 200 μm NBD labeled peptide solutions (in Tris buffer) was mixed with 180 μL of fresh cell culture EMEM medium containing 1 mm of glutathione. After incubation at 37 °C for 2 h or 8 h, the culture medium was removed and washed extensively three times with PBS buffer. For groups with CR staining, the CR stock solution was added to the cell culture to reach a final concentration at 20 μm and incubated for 1 h. After removing CR solution and washing three times with PBS buffer, cells were stained Hoechst 33342 at room temperature for 15 min and followed by washing three times with PBS buffer. Cells were analyzed by an A1R HD25 confocal laser scanning microscope (Nikon) and fluorescence images were processed using ImageJ.
Chen W., Li S., Lang J.C., Chang Y., Pan Z., Kroll P., Sun X., Tang L, & Dong H. (2020). Combined Tumor Environment Triggered Self-Assembling Peptide Nanofibers and Inducible Multivalent Ligand Display for Cancer Cell Targeting with Enhanced Sensitivity and Specificity. Small (Weinheim an der Bergstrasse, Germany), 16(38), e2002780.
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3

Confocal Imaging with Nikon A1R HD25

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Confocal images were collected using a Nikon A1R HD25 confocal laser scanning microscope equipped with LU-N4/N4S 4-laser unit.
Zhang S.J., Lowe L.A., Anees P., Krishnan Y., Fai T.G., Szostak J.W, & Wang A. (2023). Passive endocytosis in model protocells. bioRxiv.
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4

Subcellular Localization of Ha4CL

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To confirm the subcellular localization of Ha4CL, organelles and Ha4CL were labeled with red fluorescent protein mCherry and green fluorescent protein EGFP, respectively. Strains YH20C, YH20M, or YH23P were cultivated in 3 mL fresh SC-His-Ura media at 30 °C at 250 rpm overnight. Precultures were transferred to 25 mL of the same media with an initial OD600 of 0.1 at 30 °C, 250 rpm for 60 h. Cells were collected by centrifugation and washed twice with the same volume of phosphate buffered saline (PBS) (pH 7.4), and then diluted to an OD600 of 0.4–0.8 with PBS. Subsequently, 2.5 μL of preparations were directly plated on the slide. The subcellular localization of Ha4CL in mitochondria and peroxisomes was observed at excitation wavelengths of 488 nm for EGFP and 585 nm for mCherry using a Nikon A1R HD25 confocal laser scanning microscope combined with a Nikon TI2-E automatically inverted microscope. The images were processed and exported by NIS-Elements AR software (Nikon, Tokyo, Japan).
Meng Y., Liu X., Zhang L, & Zhao G.R. (2022). Modular Engineering of Saccharomyces cerevisiae for De Novo Biosynthesis of Genistein. Microorganisms, 10(7), 1402.
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