Chitosan (CH) (molecular weight: 190–370 kDa, degree of de-acetylation: ≥75%), Potato dextrose agar (PDA), sodium tripoly phosphate (STPP), streptomycin sulfate, phosphate buffer solution (PBS), and propidium iodide were all purchased from Sigma Aldrich (Saint Louis, MO, USA). Tween 80 (Xilong Scientific Co., Ltd., Shantou, China), sodium hypochlorite (NaOCl) (Merck, Darmstadt, Germany), acetic acid (Merck, New York, NY, USA) and the fungicide Teldor 50% SC (Bayer Crop Science Co. (Cambridge, UK) were purchased from a commercial distributer. Double-distilled water was used throughout the entire work.
Sodium hypochlorite naocl
Manufactured by Merck Group
Sourced in United States, Germany
Sodium hypochlorite (NaOCl) is a chemical compound commonly used as a disinfectant and oxidizing agent. It is a clear, greenish-yellow liquid with a characteristic chlorine-like odor. Sodium hypochlorite is a versatile lab equipment product that can be used in various applications due to its strong oxidizing properties.
Automatically generated - may contain errors
Lab products found in correlation
Tween 80, by Xilong (3 mentions)
Potato dextrose agar (pda), by Merck Group (1 mentions)
Phosphate buffer solution (pbs), by Merck Group (1 mentions)
Sodium tripolyphosphate, by Merck Group (1 mentions)
Streptomycin sulfate, by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Alanine, by Merck Group (1 mentions)
Iron 2 chloride, by Merck Group (1 mentions)
Acetic acid ch3cooh, by Merck Group (1 mentions)
Cysteine, by Merck Group (1 mentions)
Na2hpo4, by Merck Group (1 mentions)
Nah2po4, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Phenazine methosulfate, by Merck Group (1 mentions)
Sabouraud dextrose broth (sdb), by Merck Group (1 mentions)
Morpholinopropanesulfonic acid mops, by Merck Group (1 mentions)
β nicotinamide adenine dinucleotide nadh, by Merck Group (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
13 protocols using sodium hypochlorite naocl
1
Chitosan-Based Antimicrobial Formulation
2
Cysteine and Alanine Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cysteine (98.8%), alanine (97.6%), NaH2PO4 (AR), and Na2HPO4 (AR) were procured from Sigma-Aldrich. Sodium hypochlorite (NaOCl, AR), iron(ii ) chloride (AR), 1,10-ortho-phenanthroline (AR), hydrochloric acid (HCl, AR), acetic acid (CH3COOH, AR), and sodium hydroxide (NaOH, AR) were procured from Merck.
3
Comprehensive Antioxidant Assays Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Gallic acid, sodium carbonate (Na2CO3), catechin, nitrotetrazolium blue chloride (NBT), ascorbic acid, 2,2′-azinobis-3-ethylbenzothiozoline-6-sulfonic (ABTS), potassium persulfate (K2S2O8), dihydrorodamine (DHR), and sodium hypochlorite (NaOCl) were purchased from Sigma-Aldrich, Taufkirchen, Germany. Ferric chloride (FeCl3), sodium hydroxide (NaOH), dimethylformamide (DMF), disodium (Na2HPO4), monopotassium phosphate (KH2PO4) phenol reagent appropriate for Folin-Ciocalteu (Folin) and Sabouraud dextrose broth (SDA) were obtained from Merck, Darmstadt, Germany. 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), ferrous sulfate, β-nicotinamide adenine dinucleotide (NADH), and phenazine methosulfate (PMS) were purchased from Sigma-Aldrich, Buchs (Switzerland), Anekal Taluk (India), and Burlington, VT, (USA), respectively. Sodium acetate, RPMI 1640 and morpholinopropanesulfonic acid (MOPS) was sourced from Sigma Chemical Co., Burlington, VT, USA, acetic acid from Chem-Lab NV, Zedelgem, Belgium, and anhydrous absolute ethanol from Carlo Erba Reagents, Val-de-Reuil, France. Ultra-pure water was obtained in-house using a Milli-Q water purification system (TGI Pure Water Systems, San Diego, CA, USA). All other chemicals were of analytical grade or equivalent.
4
Characterization of Human LDL Particles
5
Isolation and Characterization of Native Myeloperoxidase
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sodium hypochlorite (NaOCl), tert-butyl hydroperoxide solution, and reduced glutathione were from Sigma-Aldrich (St. Louis, MO). ThioGlo 1 (10-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)-9-methoxy-3-oxo-3H-naphthol[2,1-b]pyran-2-carboxylic acid methyl ester) and DEPMPO (5-diethoxy-phosphoryl-5-methyl-1-pyrroline N-oxide) were from Calbiochem (San Diego, CA). Other reagents were from Sigma-Aldrich (USA).
The concentration of commercial NaOCl solutions was determined as OClˉ concentration measured spectrophotometrically at pH 12.0, taking the molar extinction coefficient (ε292) equal to 350 M−1 cm−1 [34 (link)]. Assuming that рKα for HOCl is ~7.5 [34 (link)] and that at physiological pH about 50% of HOCl exists in the protonated form, while the resting 50% is in the dissociated form, hereinafter under the term “HOCl” is understood the HOCl/OClˉ mixture present in the test solution. The working solution of HOCl was prepared immediately before assay by dissolution of the commercial preparation in 10 mM Na-phosphate buffer pH 7.4 containing 140 mM NaCl.
Native myeloperoxidase (MPO) was isolated from extracts of frozen leukocytes of healthy donors as described elsewhere [35 (link)]. The purity of MPO preparations was estimated by the Reinheit Zahl (RZ) value (the 430/280 nm absorbance ratio ~0.85).
The concentration of commercial NaOCl solutions was determined as OClˉ concentration measured spectrophotometrically at pH 12.0, taking the molar extinction coefficient (ε292) equal to 350 M−1 cm−1 [34 (link)]. Assuming that рKα for HOCl is ~7.5 [34 (link)] and that at physiological pH about 50% of HOCl exists in the protonated form, while the resting 50% is in the dissociated form, hereinafter under the term “HOCl” is understood the HOCl/OClˉ mixture present in the test solution. The working solution of HOCl was prepared immediately before assay by dissolution of the commercial preparation in 10 mM Na-phosphate buffer pH 7.4 containing 140 mM NaCl.
Native myeloperoxidase (MPO) was isolated from extracts of frozen leukocytes of healthy donors as described elsewhere [35 (link)]. The purity of MPO preparations was estimated by the Reinheit Zahl (RZ) value (the 430/280 nm absorbance ratio ~0.85).
6
Root Canal Preparation and Irrigation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Teeth were sectioned apically 12 mm below and coronally 2 mm above the cemento-enamel junction with a low-speed rotary saw (Isomet, Buhler, Lake Bluff, IL, USA). Tissue remnants were removed using a size 20 Hedström file (Dentsply Maillefer, Ballaigues, Switzerland)—samples were instrumented with rotary files (ProTaper, Dentsply, Maillefer, Ballaigues, Switzerland) to standardize the master apical file to be size 40 (F4). Next, Peeso reamers (Maillefer, Ballaigues, Switzerland) from #1 to #5 were used sequentially to obtain larger root canals.
Between the uses of each file, the root canals were irrigated with 2 mL of 2.5 % sodium hypochlorite (NaOCl) (Sigma-Aldrich, St. Louis, MO). Final irrigation was performed using 5 mL of 2.5 % NaOCl (1 min) and 5 mL of 17 % EDTA (Sigma) (1 min). Next, the canals were rinsed with sterile distilled water and then dried with paper points (Meta, Metabiomed, Chungbuk, Korea).
Between the uses of each file, the root canals were irrigated with 2 mL of 2.5 % sodium hypochlorite (NaOCl) (Sigma-Aldrich, St. Louis, MO). Final irrigation was performed using 5 mL of 2.5 % NaOCl (1 min) and 5 mL of 17 % EDTA (Sigma) (1 min). Next, the canals were rinsed with sterile distilled water and then dried with paper points (Meta, Metabiomed, Chungbuk, Korea).
7
Instrumentation of Immature Apices
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Teeth were sectioned apically 12 mm below and coronally 2 mm above the cementoenamel junction using a diamond disk. Remnant pulpal soft tissue was removed using a size 30 Hedström file (Dentsply Maillefer, Ballaigues, Switzerland) [Figure 1 ]. Working length was determined radiographically using size 20 K-files (Dentsply Maillefer, Ballaigues, Switzerland) and was kept 2 mm more than normal to over-instrument the canals simulating immature open apices. Rotary instrumentation was carried out using ProTaper rotary instruments (Dentsply Maillefer, Ballaigues, Switzerland) to standardize to master apical size 30 corresponding to F-3. Next Peeso reamers (Dentsply Maillefer, Ballaigues, Switzerland) were used sequentially from #1 to #5 to obtain larger canal space and foramen. The canals were irrigated with 2 mL 2.5% sodium hypochlorite (NaOCl) (Sigma-Aldrich, St. Louis, MO) between the instrument changes. A final flush was applied using 5 mL 17% ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich, St. Louis, MO) for 1 min and 5 mL 2.5% NaOCl for 1 min and then dried using paper points (Dentsply Maillefer, Ballaigues, Switzerland)
8
Melatonin Synthesis and Purification
9
Virucidal Activity Screening of D. canadense
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Testing solutions were prepared by mixing BoHV-1 (from 10 8.0 to 10 8.75 TCID 50 /ml) suspensions in DMEM:F12 with 2% FBS with previously established noncytotoxic extract volumes. The virus and extract mixtures were incubated at 20°C for 1 hour and titrated as described above. Controls of cells, the virus and extracts were included. After 96 h of incubation, the plates were examined using an inverted microscope (Leica, Germany) to detect CPE.
Sodium hypochlorite (NaOCl -Sigma-Aldrich, USA) in a final concentration of 0.05-0.1% was used as the positive control. NaOCl was diluted in water of standard hardness to a stock concentration. Sodium thiosulphate (Sigma-Aldrich, USA) in distilled water was used to neutralise the activity of NaOCl.
Based on the results of screening for virucidal activity, the extract of D. canadense was chosen for further investigation.
Sodium hypochlorite (NaOCl -Sigma-Aldrich, USA) in a final concentration of 0.05-0.1% was used as the positive control. NaOCl was diluted in water of standard hardness to a stock concentration. Sodium thiosulphate (Sigma-Aldrich, USA) in distilled water was used to neutralise the activity of NaOCl.
Based on the results of screening for virucidal activity, the extract of D. canadense was chosen for further investigation.
10
Sodium Hypochlorite Characterization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sodium hypochlorite (NaOCl) was purchased from Sigma-Aldrich (St. Louis, MO). NaOCl concentration was determined using an extinction coefficient of 350 M−1 cm−1 at 292 nm in water. Zeba Spin desalting columns and Amicon Ultra centrifugal filters were purchased from Thermo Fisher Scientific (Waltham, MA) and Millipore Sigma (Burlington, MA), respectively. Helixate FS (CSL Behring, Kankakee, IL, USA; Lots 270PP4J and 27N1VK1) was purchased from the manufacturer and reconstituted using sterile water in accordance with the kits and instructions provided.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!