Biocoat inserts

Manufactured by BD

Sourced in Germany, United States

BioCoat™ inserts are a type of cell culture labware designed for in vitro studies. They feature a coated membrane that facilitates cell attachment and growth. The core function of BioCoat™ inserts is to provide a specialized surface for culturing cells.

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Lab products found in correlation

Uncoated polycarbonate inserts, by Merck Group (6 mentions) Counting kit cck 8, by Dojindo Laboratories (1 mentions) Cell counting kit 8, by Dojindo Laboratories (1 mentions) Cell counting kit, by Dojindo Laboratories (1 mentions) 5 ethynyl 2 deoxyuridine (edu), by RiboBio (1 mentions)

Close spelling variants of this product

BioCoat cell culture inserts BD Biocoat control inserts BD Biocoat cell inserts BD BioCoat Matrigel Invasion Chambers and Control Inserts BD BioCoat Millicell inserts BD BioCoat™ 8.0 μm PET Membrane 24-well Cell Culture Inserts BD BioCoat Matrigel Invasion inserts Transwell Biocoat control inserts 24-well BioCoat cell culture inserts BioCoatTM Matrigel chamber inserts

8 protocols using biocoat inserts

Cell Proliferation, Migration, and Invasion Assays

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Cell counting kit (CCK-8; Dojindo, Kumamoto, Japan) assays were used to detect cell proliferation. Migration was examined by both a wound-healing assay and a Transwell assay (uncoated insert). A Transwell assay (Matrigel-coated insert) was used for invasion monitoring. Cell migration and invasion assays were performed using a Transwell technique with uncoated polycarbonate inserts (Millipore, Darmstadt, Germany) or BioCoat™ inserts (BD Biosciences, NJ, USA). Medium without FBS (2 × 10⁴ cells/200 μL) was added to the upper portion of a migration (uncoated insert) or invasion (Matrigel-coated insert) chamber, with 600 μL of DMEM containing 10% FBS added to the lower chamber. All experiments were performed three times, and the cell numbers were counted at least three times to calculate an average.

Cheng A., Xu Q., Li B., Zhang L., Wang H., Liu C., Han Z, & Feng Z. (2024). The enhanced energy metabolism in the tumor margin mediated by RRAD promotes the progression of oral squamous cell carcinoma. Cell Death & Disease, 15(5), 376.

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Cell Migration and Invasion Assays

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Migration ability was examined by both a wound healing assay and a transwell assay (uncoated insert). 1–6 × 10⁴ cells were seeded in BioCoat™ inserts (BD Biosciences, Franklin Lake, NJ, USA) for invasion. The migrating and invading cells were stained using 5% crystal violet. All the experiments were performed in triplicate.

Chang H., Xu Q., Li J., Li M., Zhang Z., Ma H, & Yang X. (2021). Lactate secreted by PKM2 upregulation promotes Galectin-9-mediated immunosuppression via inhibiting NF-κB pathway in HNSCC. Cell Death & Disease, 12(8), 725.

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Functional Assessments of lincRNA-p21

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HN6 and Cal27 cells were transfected with siRNA or plasmids of lincRNA-p21 for 24 h, and then seeded in the plates. As described in our previous study [25 (link)], cell proliferation experiments were performed using the Cell Counting Kit (CCK8; Dojindo, Kumamoto, Japan) assays. The colony-forming assay was performed to monitor the cloning capability of HN6 and Cal27 cells [22 (link)]. Cell migration and invasion assays were performed using a Transwell technique with uncoated polycarbonate inserts (Millipore, Darmstadt, Germany) or BioCoat™ inserts (BD Biosciences, Franklin Lake, NJ, USA). Medium without FBS (2 × 10⁴ cells/200 μl) were added into the upper portion of a migration (uncoated insert) or invasion (matrigel-coated insert) chamber, with 500 μl DMEM containing 10% FBS added into the lower chamber. After crystal violet staining, the crossed cells were dissolved in 33% of acetic acid. OD 570 nm absorbance was measured.

Jin S., Yang X., Li J., Yang W., Ma H, & Zhang Z. (2019). p53-targeted lincRNA-p21 acts as a tumor suppressor by inhibiting JAK2/STAT3 signaling pathways in head and neck squamous cell carcinoma. Molecular Cancer, 18, 38.

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Transwell Assay for Cell Migration and Invasion

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Cell migration and invasion assays were performed using Transwell assay with uncoated polycarbonate inserts (Millipore, Darmstadt, Germany) for migration or BioCoat™ inserts (BD Biosciences, Franklin Lake, NJ, USA) for invasion. 1 ~ 5 × 10⁴ transfected cells were seed in the upper chamber. After crystal violet staining, the positive cells were counted and analyzed under microscope.

Ma H., Chang H., Yang W., Lu Y., Hu J, & Jin S. (2020). A novel IFNα-induced long noncoding RNA negatively regulates immunosuppression by interrupting H3K27 acetylation in head and neck squamous cell carcinoma. Molecular Cancer, 19, 4.

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Transwell Migration and Invasion Assay

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Cell migration and invasion assays were performed using Transwell technique with uncoated polycarbonate inserts (Millipore) for migration or BioCoat™ inserts (BD Biosciences) for invasion. Medium without FBS (1–5 × 10⁴ cells/200 μL) was added into the upper chamber, with 500 μL DMEM containing 10% FBS added into the lower chamber. After crystal violet staining, the positive cells were counted and analyzed under microscope.

Yang L., Chen Y., Liu N., Shi Q., Han X., Gan W, & Li D. (2021). Low expression of TRAF3IP2-AS1 promotes progression of NONO-TFE3 translocation renal cell carcinoma by stimulating N6-methyladenosine of PARP1 mRNA and downregulating PTEN. Journal of Hematology & Oncology, 14, 46.

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Analyzing Cell Migration and Invasion

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Transwell assays were performed to analyze cell migration and invasion. Uncoated polycarbonate inserts (Millipore) were used for the migration experiment, and BioCoat™ inserts (BD Biosciences, Franklin Lake, NJ, USA) were used for the invasion experiment. Transfected CAL27 and HN6 cells were seeded in the upper chamber. After 24 h, the cells were stained with crystal violet, and the number of crystal violet‐positive cells on the membrane was counted in three randomly selected fields of view at a magnification of 100×. For the wound healing assay, cells were seeded in 6‐well plates, and a 20‐μL pipette tip was utilized to scratch a uniform linear wound in the cell monolayer. Cell migration across the wound was observed at 0 h and 24 h after scraping, and fields of view were randomly selected and photographed.

Ju H., Hu Z., Wei D., Huang J., Zhang X., Rui M., Li Z., Zhang X., Hu J., Guo W, & Ren G. (2021). A novel intronic circular RNA, circGNG7, inhibits head and neck squamous cell carcinoma progression by blocking the phosphorylation of heat shock protein 27 at Ser78 and Ser82. Cancer Communications, 41(11), 1152-1172.

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Cell Proliferation, Migration, and Invasion Assay

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Cell proliferation was detected using a Cell Counting Kit (CCK8; Dojindo, Kumamoto, Japan). Cells were seeded into 6-well plates at 500 cells/well for 10 ~ 14 days to analyze the clone-forming capacity. Transwell assays were performed using uncoated polycarbonate inserts (Millipore, Darmstadt, Germany) to test migration or BioCoat™ inserts (BD Biosciences, Franklin Lake, NJ, USA) to test invasion. EdU (RiboBio, Guangzhou, China) and TUNEL (Beyotime, Shanghai, China) staining were conducted according to the manufacturers’ protocols.

Jin S., Li M., Chang H., Wang R., Zhang Z., Zhang J., He Y, & Ma H. (2022). The m6A demethylase ALKBH5 promotes tumor progression by inhibiting RIG-I expression and interferon alpha production through the IKKε/TBK1/IRF3 pathway in head and neck squamous cell carcinoma. Molecular Cancer, 21, 97.

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Assessing Tumor Cell Migration and Invasion

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We assessed the migration and invasion of tumor cells using a transwell assay. For migration, we used uncoated polycarbonate inserts (Millipore), while we used BioCoat™ inserts (BD Biosciences) for invasion. After starving the cells overnight, we filled the upper chamber with 1–5 × 10⁴ cells suspended in DMEM without FBS and the lower chamber with 500 μL of DMEM containing 10% FBS. After crystal violet staining, we counted and analyzed the positive cells under a microscope.

Yang L., Yin H., Chen Y., Pan C., Hang H., Lu Y., Ma W., Li X., Gan W., Guo H, & Li D. (2022). Low expression of PEBP1P2 promotes metastasis of clear cell renal cell carcinoma by post-transcriptional regulation of PEBP1 and KLF13 mRNA. Experimental Hematology & Oncology, 11, 87.

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