The UAS-Datlastin-myc and UAS-BiP-sfGFP-HDEL fly lines used in this study were described previously (Orso et al., 2009 (link); Summerville et al., 2016 (link)). The Gal4 strains used were: tubulin-Gal4, elav-Gal4, D42-Gal4, and GMR-Gal4 obtained from Bloomington Drosophila Stock Center. To increase protein expression, experimental crosses were performed at 28°C, except for crosses with GMR-Gal4 which were performed at 25°C. Control genotypes included promoter-Gal4/+ individuals. Fly food was prepared using NUTRI-fly-IF mixture (Genesee Scientific), according to the manufacturer instructions.
Nutri fly if mixture
Manufactured by Genesee Scientific
NUTRI-fly-IF mixture is a nutrient-rich medium designed for the maintenance and growth of Drosophila (fruit fly) cultures. It provides essential nutrients and components required for the optimal development and reproduction of fruit flies in a laboratory setting.
Automatically generated - may contain errors
2 protocols using nutri fly if mixture
1
Fly Genetic Manipulation Protocols
2
Expression and Treatment of Drosophila Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The UAS-Dspastin-K467R and UAS-BiP-sf-GFP-ER fly lines used in this study were described previously (Orso et al., 2005 (link); Summerville et al., 2016 (link)). The Gal4 strains used were: Elav-Gal4 (pan neuronal expression); D42-Gal4 (motor neurons restricted), obtained from Bloomington Drosophila Stock Center. To increase protein expression, all experimental crosses were performed at 28°C. Control genotypes included promoter-Gal4/+ individuals. Fly food was prepared using NUTRI-fly-IF mixture (Genesee Scientific), according to the manufacturer instructions. For chronic vinblastine treatment, NUTRI-fly-IF was additioned with 50 nM vinblastine.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!