The compounds were analyzed with a Shimadzu HPLC system on a Phenomenex C18 Gemini column (5 μm, 4.6 × 250 mm) equipped with a binary pump (SPD-20AD), a UV detector (SPD-20A), an autosampler (SIL-20A), a column oven (CTO-20AC), a degasser, (DGU-20A3) and an LC solution system (Shimadzu, Kyoto, Japan). The flavonoid and phenolic compound quantification was conducted using a calibration curve at ten concentrations over a linear range (0.0005–1.0 mg/mL), and the chromatographic analysis was performed using the method previously reported by Jin et al. [13 (link)].
Lc solution system
Manufactured by Shimadzu
Sourced in Japan
The LC Solution system is a liquid chromatography (LC) platform designed for analytical and preparative applications. It provides basic functionality for LC analysis, including solvent delivery, sample injection, and detection. The system is capable of performing various LC techniques such as reversed-phase, normal-phase, and ion exchange chromatography. It is intended to serve as a reliable and versatile tool for chemical separation and analysis in a laboratory setting.
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2 protocols using lc solution system
1
HPLC Analysis of Flavonoid and Phenolic Compounds
2
HPLC Analysis of Radioactive Compounds
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HPLC analysis was performed in a Shimadzu LCsolution system equipped with a photodiode array detector (UV-Visible) connected in series with a gamma detector (Lablogic). As the stationary phase a C18 column, 5 μm, 11 nm pore size, 150 x 4.6 mm, Macherey-Nagel (Nucleodur 100-5 C18 ec) was used. It was eluted with a solvent system consisting of 0.1% TFA in water (A) and 0.1% TFA in acetonitrile (B) with the following optimized gradient: 0-18 min (20-38% B), 18-20 min (38-20% B), 20-25 min (20% B) at a flow rate of 1 mL.min-1. The activity percentage of each peak was calculated as the percentage of the area present under each peak relative to the total integrated area (all combined radioactive peaks plus any segments of elevated baseline).
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