2487 dual absorbance detector system

Manufactured by Waters Corporation

The 2487 dual-absorbance detector system is a laboratory instrument designed for the detection and measurement of absorbance in liquid chromatography applications. The system features two independent absorbance detection channels, allowing for the simultaneous monitoring of two wavelengths. The 2487 provides accurate and reliable absorbance data to support analytical methods and workflows.

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Lab products found in correlation

1525 binary hplc pump, by Waters Corporation (2 mentions) Sunfire c18 obd 5 μm 19 150 mm column, by Waters Corporation (1 mentions) Symmetry c18 5 μm 4.6 150 mm column, by Waters Corporation (1 mentions) Microflex, by Bruker (1 mentions) 600 mhz nmr, by Bruker (1 mentions) 400 mhz nmr, by Bruker (1 mentions)

Close spelling variants of this product

2487 Dual Absorbance Detector (16 citations) Waters 2487 dual λ absorbance detector system (4 citations)

2 protocols using 2487 dual absorbance detector system

Preparative and Analytical HPLC Purification

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Preparative HPLC was performed using a Waters 1525 Binary HPLC pump equipped with a Waters 2487 dual-absorbance detector system and a Waters Sunfire C18 OBD 5-μm 19 × 150 mm column. Absorbance was monitored at 220 and 345 nm. A gradient of 20–100% methanol in H₂O with 0.1% trifluoroacetic acid (TFA) over 60 min was used for compound purification. Analytical HPLC was performed using a Waters Symmetry C18 5 μm 4.6 × 150 mm column. Compounds were analysed using a gradient of 20–60% MeOH in H₂O with 0.1% TFA over 30 min. All compounds evaluated had ≥95% purity as determined by analytical HPLC. Mass spectrometry was performed with an Applied Biosystems MALDI ToF/ToF Analyzer 4800 Plus and Microflex (Bruker) using an α-hydroxycinnamic acid matrix. See the Supplementary Information for details of compound synthesis and compound characterization.

Yang W.Y., Gao R., Southern M., Sarkar P.S, & Disney M.D. (2016). Design of a bioactive small molecule that targets r(AUUCU) repeats in spinocerebellar ataxia 10. Nature Communications, 7, 11647.

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Purification and Characterization of Small Molecules

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Microwave reactions were carried out using a Biotage Initiator+ SP Wave microwave. Compounds were purified by preparative reverse phase HPLC using a Waters 1525 Binary HPLC pump equipped with a Waters 2487 dual absorbance detector system and a Waters Sunfire C18 OBD 5 μm 19 x 150 mm column. Absorbance was monitored at 345 and 220 nm. A gradient of 0-100% MeOH in H₂O with 0.1% TFA over 60 min was used for compound purification. Purity was assessed by analytical HPLC using a Waters Symmetry C18 5 μm 4.6 x 150 mm column. Small molecules were analyzed using a gradient of 0-100% methanol in water with 0.1% TFA over 60 min. ¹H NMR spectra were collected using a Bruker 400 MHz NMR, while ¹³C NMR spectra were collected on a Bruker 600 MHz NMR. High resolution mass spectrometry (HR-MS) was completed on an Applied Biosystems MALDI ToF Analyzer 4800 Plus using an α-cyano-4-hydroxycinnamic acid matrix with calibration standards. All compounds evaluated had ≥95% purity.

Angelbello A.J., Benhamou R.I., Rzuczek S.G., Choudhary S., Tang Z., Chen J.L., Roy M., Wang K.W., Yildirim I., Jun A.S., Thornton C.A, & Disney M.D. (2020). A small molecule that binds an RNA repeat expansion stimulates its decay via the exosome complex. Cell chemical biology, 28(1), 34-45.e6.

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