Hcc1419

MCF10A, ZR75-30, MDA-MB-453, BT20, JIMT-1, MDA-MB-231, and HEK293T cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). MCF7, T47D, ZR75-1, SKBR3, BT474, HCC-1954, HCC1419, and HS578T cells were purchased from the Korean Cell Line Bank. MCF7, T47D, MDA-MB-231, and HEK293T cells were cultured in DMEM (Welgene, Daegu, Republic of Korea) supplemented with 10% FBS, and SKBR3, BT474, HCC-1954, HCC-1419, JIMT-1, and HS5788T cells were cultured in RPMI (Welgene) supplemented with 10% FBS at 37 °C in a 5% CO2 atmosphere. Trastuzumab was obtained from Roche (Basel, Switzerland). The β-Catenin/TCF inhibitor FH-535 was purchased from Sigma-Aldrich (St. Louis, MO, USA).

The human normal breast cell line, MCF10A, was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). These cells were incubated and cultured in Dulbecco’s modified Eagle medium nutrient mixture F-12 (DMEM/F12, Gibco, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS, Welgene, Gyeongsan, Republic of Korea), epidermal growth factor (EGF; 20 ng/mL, Invitrogen, Waltham, MA, USA), hydrocortisone (0.5 μg/mL, Sigma), insulin (0.01 mg/mL, Sigma) 100 units/mL penicillin (Gibco), and 100 mg/mL streptomycin (Gibco). Human breast cancer cell lines (MCF7, SK-BR-3, T47D, HCC1419, HT20, and MDAMB231) were purchased from the Korean Cell Line Bank (Cancer Research Center, Seoul National University, Seoul, Republic of Korea). These cells were cultured in DMEM (Gibco, Waltham, MA, USA) supplemented with 10% FBS (Welgene, Gyeongsan, Republic of Korea), 100 units/mL penicillin (Gibco), and 100 mg/mL streptomycin (Gibco). The cells were incubated in a humidified incubator with 5% CO₂ at 37 °C.