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7 protocols using cobalt 2 sulfate heptahydrate

1

Metal Ion Supplementation Protocol

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Puromycin Dihydrochloride (>99%) was purchased from P212121. Zinc Sulfate was purchased as a 100 mM aq. Soln. from Alfa Aesar. Cobalt(II) sulfate heptahydrate (>99%) was purchased from Sigma-Aldrich: SIAL. Magnesium(II) chloride hexahydrate (ACS grade, >99%) was purchased from Amresco. Ferrous ammonium sulfate (99%) was purchased from Santa Cruz Biotechnology. Sodium chloride (99.5%) was purchased from Fisher Scientific. Nickel(II) sulfate (99.99%), Fe(II) bromide (98%), Fe(III) sulfate hydrate (97%), copper(II) chloride (99.999%), copper(I) chloride (>99.995%), calcium chloride (>97%), potassium chloride (>99%), and manganese(II) chloride (98%) were all purchased from Sigma Aldrich. N-acetyl cysteine (NAC) was purchased from TCI America (>98%). Ascorbic Acid was purchased from Chem Impex Int’l (99.8%). Buthionine sulphoximine (BSO) (>98%) was purchased from Cayman Chemical and β-mercaptoethanol (BME) (>98%) was purchased from BioRad. Hepcidin was purchased from abcam (1 mg/mL, 70–90%).
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2

Synthesis of Cobalt Nanoparticles

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Cobalt (II) sulfate heptahydrate (CoSO4·7H2O), DA hydrochloride, trizma hydrochloride (Tris-HCl), terephthalic acid (TA), sodium acetate (NaAc), sodium borohydride (NaBH4), and sodium sulfide (Na2S) were purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.). Hydrogen peroxide was obtained from Samchun Chemical (Seoul, Korea). All solutions were prepared in deionized water purified using a Milli-Q Purification System (Millipore, Billerica, MA, U.S.A.).
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3

Synthesis of Metal-Organic Complexes

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The following reagents were purchased from Sigma-Aldrich: nickel(II) sulfate hexahydrate (≥98%), cobalt(II) sulfate heptahydrate (≥99%), adenosine 5’-diphosphat sodium salt (ADP, ≥95%, bacterial, HPLC), sodium carbonate (≥99.5%), potassium acetate (98%), potassium nitrate (99%), potassium iodide (99%), calcium dichloride hexahydrate (98%), sodium citrate tribasic dihydrate, L-cysteine (97%), ethylenediaminetetraacetic acid tetrasodium salt hydrate (EDTA), sodium pyrophosphate (98%), DOC (BioXtra, ≥98.0%), SDS (≥99.0%), sodium cholate (SC, ≥99.0%), sodium hypochlorite (NaClO, 10–15% active chlorine), tetrafluoroboronic acid (48 wt.%), tert-butyl nitrite (90%), 4-ethynylaniline (97%), 2-ethynylaniline (98%), aniline (≥99.5%), CuSO4(H2O)5 (≥99.9%). The following reagents were purchased from TCI: adenosine 5’-triphosphate disodium hydrate (ATP, >98%, HPLC), dextran (Mw = 70 kg mol−1), THPTA (>97%). Poly(ethylene glycol) (PEG, Mw = 6,000 g mol−1) was purchased from Alfa Aesar. PL-PEG5000 (18:0 PEG5000PE, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)−5000], Mw = 5801.071 g mol−1) was purchased from Avanti Polar Lipids, Inc.
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4

Metal Ion Supplementation Protocol

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Puromycin Dihydrochloride (>99%) was purchased from P212121. Zinc Sulfate was purchased as a 100 mM aq. Soln. from Alfa Aesar. Cobalt(II) sulfate heptahydrate (>99%) was purchased from Sigma-Aldrich: SIAL. Magnesium(II) chloride hexahydrate (ACS grade, >99%) was purchased from Amresco. Ferrous ammonium sulfate (99%) was purchased from Santa Cruz Biotechnology. Sodium chloride (99.5%) was purchased from Fisher Scientific. Nickel(II) sulfate (99.99%), Fe(II) bromide (98%), Fe(III) sulfate hydrate (97%), copper(II) chloride (99.999%), copper(I) chloride (>99.995%), calcium chloride (>97%), potassium chloride (>99%), and manganese(II) chloride (98%) were all purchased from Sigma Aldrich. N-acetyl cysteine (NAC) was purchased from TCI America (>98%). Ascorbic Acid was purchased from Chem Impex Int’l (99.8%). Buthionine sulphoximine (BSO) (>98%) was purchased from Cayman Chemical and β-mercaptoethanol (BME) (>98%) was purchased from BioRad. Hepcidin was purchased from abcam (1 mg/mL, 70–90%).
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5

Preparation and Characterization of Metal Salt Solutions

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Iron (II) sulfate heptahydrate (FeSO4·7H2O or FeSO4 (aq)) and iron (III) nitrate nonahydrate (Fe(NO3)3·9H2O) were purchased from Carl Roth GmbH + Co. KG, Karlsruhe, Germany. Nickel(II) sulfate hexahydrate (NiSO4·6H2O), copper (II) sulfate (CuSO4), cobalt (II) sulfate heptahydrate (CoSO4·7H2O), iron (II)-chloride (FeCl2), copper (II) chloride dihydrate (CuCl2·2H2O), copper (II) nitrate hemi(pentahydrate) (Cu(NO3)2·2.5H2O), zinc (II) nitrate hydrate (Zn(NO3)2·H2O), nickel (II) chloride hexahydrate (NiCl2·6H2O), nickel (II) nitrate hexahydrate (Ni(NO3)2·6H2O), cobalt (II) chloride hexahydrate (CoCl2·6H2O), cobalt (II) nitrate hexahydrate (Co(NO3)2·6H2O), manganese (II) chloride (MnCl2), manganese (II) nitrate tetrahydrate (Mn(NO3)2·4H2O), and zinc(II) sulfate monohydrate (ZnSO4·H2O) were purchased from Sigma-Aldrich Chemie GmbH, Steinheim, Germany. Manganese (II) sulfate monohydrate (MnSO4·H2O) and zinc (II) chloride (ZnCl2) were purchased from Merck KGaA, Darmstadt, Germany. By default, all solutions and any flushing processes were made with deionized water from a Milli-Q-Advantage A10 from Merck Millipore (Darmstadt, Germany). The produced deionized water is ultrapure water from purity degree of type 1, which was added with pretreated water. It is characterized by a constant conductivity of 0.055 µS·cm−1, respectively 18.2 MΩ·cm, at a temperature of 298 K.
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6

Spectrophotometric Determination of Antioxidant Capacity

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The
instrumental and equipment resources are as follows: a Varian CARY
100 UV–vis spectrophotometer (Mulgrave, Victoria, Australia)
equipped with matched HELLMA quartz cuvettes with a 10 mm light path,
a Radwag AS 220/C/2 analytical balance (Radom, Poland), a Sonorex
ultrasonic bath (Bandelin, Germany), a Velp Scientifica ZX3 Advanced
Vortex Mixer (Usmate, Italy), a Memmert water bath WNB 7-45 (Schwabach,
Germany), and a HANNA HI 221 pH meter (Woonsocket, RI-USA).
Chemicals used in this study were of analytical grade purity and
were purchased from different sources. Cobalt(II) sulfate heptahydrate,
sodium sulfite, disodium hydrogen phosphate dihydrate, sodium dihydrogen
phosphate dihydrate, and ammonium chloride were supplied by Merck;
acetic acid, sodium acetate, ammonia, ethanol (EtOH), methanol (MeOH),
acetonitrile, acetone, tert-butanol, 3,3′,5,5′-tetramethylbenzidine
(TMB), gallic acid (GA), ferulic acid (FA), catechin (CAT), epicatechin
(EC), ascorbic acid (AA), l-cysteine (CYS), caffeic acid
(CFA), glutathione (GSH), NAC, quercetin (QR), p-coumaric
acid (p-CUM), and naringenin (NG) were from Sigma-Aldrich;
and rhodamine B was from Sigma.
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7

Synthesis of Transition Metal Oxides

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Cobalt(II) sulfate heptahydrate (CoSO4·7H2O; ≥99% purity, CAS No: 10026-24-1, EC number: 233-334-2), nickel sulfate hexahydrate (NiSO4·6H2O; CAS No: 1010-97-0, EC number: 232-104-9), vanadium pentoxide (V2O5; 99.95%, CAS No: 1314-62-1, EC number: 215-239-8), sodium hydroxide (NaOH; CAS No: 1310-73-2, EC number: 215-185-5), sodium nitrate (NaNO3; 99.99% Suprapur, CAS No: 7631-99-4, EC number: 231-554-3), propanone (CH3COCH3; ≥99.5%, CAS No: 67-64-1, EC number: 200-662-2), potassium hydroxide (KOH; CAS No: 1310-58-3, EC number: 215-181-3), and hydrochloric acid (HCl; 37%) were purchased from Merck (Darmstadt, Germany). The Ni Foams (100 × 100 mm2; thickness 1.6 mm; pore size 200–300 μm) were purchased from MTI (Seoul, South Korea). All deionized water used in the synthesis procedure was obtained from our in-house Merck Millipore water purification system (Darmstadt, Germany).
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