Microalgae cells were immobilized between two filter membranes. Lower filter membrane faced with oxygen sensor array should insulate light in order to protect fluorescence dye from light for photosynthesis. Therefore, we used black MCE filter membrane. Upper filter should pass light in order to accelerate photosynthesis. We employed Omnipore® filter membrane which become half-transparent in wet condition.
The illustration inFigure 2 shows the orderly procedure of microalgae immobilization.
The immobilization procedure was very simple, that is, onto an MCE membrane (diameter 25 mm, pore size 0.45 μm, black type, A045N025A, Advantec, Tokyo, Japan), a space (5 × 5 mm2) was created by attaching double adhesive tape, and around 20 μL cell suspension (3 mg cells) was dripped into the space, aiming for an equal cell density in all parts of the space. After that, an Omnipore® membrane (diameter 13 mm, pore size 1.0 μm, white type, 2-305201, Millipore, Burlington, MA, USA) was placed just above the cell layer and attached closely to the double adhesive tape. The cells were trapped between MCE and Omnipore® filter membranes and this is referred to as the biofilm.
The illustration in
The immobilization procedure was very simple, that is, onto an MCE membrane (diameter 25 mm, pore size 0.45 μm, black type, A045N025A, Advantec, Tokyo, Japan), a space (5 × 5 mm2) was created by attaching double adhesive tape, and around 20 μL cell suspension (3 mg cells) was dripped into the space, aiming for an equal cell density in all parts of the space. After that, an Omnipore® membrane (diameter 13 mm, pore size 1.0 μm, white type, 2-305201, Millipore, Burlington, MA, USA) was placed just above the cell layer and attached closely to the double adhesive tape. The cells were trapped between MCE and Omnipore® filter membranes and this is referred to as the biofilm.