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Cgp 55845

Manufactured by Hello Bio
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Sourced in United Kingdom

The CGP 55845 is a potent and selective GABA(B) receptor antagonist. It is commonly used in scientific research applications to investigate the role of GABA(B) receptors in various biological processes.

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Lab products found in correlation

Nbqx disodium salt, by Hello Bio (2 mentions) Dopamine, by Merck Group (2 mentions) Kynurenic acid, by Merck Group (2 mentions) Yohimbine, by Abcam (2 mentions) Citalopram, by Abcam (2 mentions) Sch 23390, by Abcam (2 mentions) Gr 127935, by Abcam (2 mentions) Kynurenic acid, by Hello Bio (2 mentions) Methysergide, by Merck Group (2 mentions) Gabazine, by Abcam (2 mentions) L dopa, by Merck Group (1 mentions) Sulpiride, by Merck Group (1 mentions) Picrotoxin, by Hello Bio (1 mentions) Forskolin, by Hello Bio (1 mentions) Mk 801, by Hello Bio (1 mentions) Dl propranolol hydrochloride, by Merck Group (1 mentions) Picrotoxin, by Merck Group (1 mentions) Dl noradrenaline hydrochloride, by Merck Group (1 mentions) Diazepam, by Bio-Techne (1 mentions) Gabazine, by Hello Bio (1 mentions)

5 protocols using cgp 55845

1

Pharmacological Modulation of Neuronal Signaling

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Drugs were bath applied. MK-801, forskolin, CGP55845, and picrotoxin were acquired from Hello Bio Princeton, NJ. CNQX, sulpiride, L-DOPA, were acquired from Sigma-Aldrich. Amphetamine was acquired from NIH NIDA and sumatriptan was acquired from Glaxo Wellcome Inc.
Robinson B.G., Cai X., Wang J., Bunzow J.R., Williams J.T, & Kaeser P.S. (2019). RIM is essential for stimulated but not spontaneous somatodendritic dopamine release in the midbrain. eLife, 8, e47972.
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2

Preparation of Neurotransmitter Compounds

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DL-noradrenaline hydrochloride and DL-propranolol hydrochloride were purchased from Sigma-Aldrich, fresh stock solutions (50 mM) were made up in dH2O each day and stored on ice. Picrotoxin (Sigma-Aldrich), CGP-55845 (HelloBio) and NBQX di-sodium salt (HelloBio) stock solutions (5 mM, 1 mM, and 20 μM, respectively) were made up in dH2O and stored at −20 °C.
Bacon T.J., Pickering A.E, & Mellor J.R. (2020). Noradrenaline Release from Locus Coeruleus Terminals in the Hippocampus Enhances Excitation-Spike Coupling in CA1 Pyramidal Neurons Via β-Adrenoceptors. Cerebral Cortex (New York, NY), 30(12), 6135-6151.
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3

Glutamatergic and GABAergic Receptor Modulation

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Drugs acting at glutamatergic and GABAergic receptors were used at the following concentrations: 50 µmol/L D‐2‐amino‐5‐phosphonovalerate (AP5) (Hello Bio, Bristol, UK); 20 µmol/L NBQX disodium salt (Hello Bio, Bristol, UK); 10 μmol/L Gabazine (Hello Bio, Bristol, UK); 3 µmol/L Diazepam (Tocris, Abingdon, UK); 5 μmol/L CGP‐55845 (Hello Bio, Bristol, UK).
Codadu N.K., Graham R.T., Burman R.J., Jackson‐Taylor R.T., Raimondo J.V., Trevelyan A.J, & Parrish R.R. (2019). Divergent paths to seizure‐like events. Physiological Reports, 7(19), e14226.
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4

Dopamine Solution Preparation and Compound Sourcing

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Dopamine solutions were made fresh daily and kept covered in aluminum foil and on ice to minimize oxidation. All other agonist and antagonists were prepared as stock solutions (1:1000-1:10,000) in DI water or dimethylsulfoxide (DMSO) and diluted into ACSF prior to experiments. The bath concentration of Rubi-GABA perfusion was 10 μM. Cocaine HCl was obtained from the National Institute on Drug Abuse. Dopamine, NEM, kynurenic acid, and methysergide were obtained from Sigma. CPP, gabazine, SCH-23390, yohimbine, citalopram, and GR 127935 were obtained from Abcam. CGP 55845 was obtained from Hello Bio Inc. NBQX was obtained from Hello Bio Inc. and Abcam. kynurenic acid was obtained from Hello Bio and Sigma. All other drugs were obtained from Tocris.
Burke D.A, & Alvarez V.A. (2022). Serotonin receptors contribute to dopamine depression of lateral inhibition in the nucleus accumbens. Cell reports, 39(6), 110795.
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5

Dopamine Solution Preparation and Compound Sourcing

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Dopamine solutions were made fresh daily and kept covered in aluminum foil and on ice to minimize oxidation. All other agonist and antagonists were prepared as stock solutions (1:1000-1:10,000) in DI water or dimethylsulfoxide (DMSO) and diluted into ACSF prior to experiments. The bath concentration of Rubi-GABA perfusion was 10 μM. Cocaine HCl was obtained from the National Institute on Drug Abuse. Dopamine, NEM, kynurenic acid, and methysergide were obtained from Sigma. CPP, gabazine, SCH-23390, yohimbine, citalopram, and GR 127935 were obtained from Abcam. CGP 55845 was obtained from Hello Bio Inc. NBQX was obtained from Hello Bio Inc. and Abcam. kynurenic acid was obtained from Hello Bio and Sigma. All other drugs were obtained from Tocris.
Burke D.A, & Alvarez V.A. (2022). Serotonin receptors contribute to dopamine depression of lateral inhibition in the nucleus accumbens. Cell reports, 39(6), 110795.
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