Analytical grade solvents

Manufactured by Avantor

Sourced in Italy, Germany

Analytical-grade solvents are high-purity organic liquids used in various laboratory applications, such as sample preparation, extraction, and analysis. They are designed to meet rigorous standards for chemical composition, purity, and consistency, ensuring reliable and accurate results in scientific research and testing.

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Lab products found in correlation

7 protocols using analytical grade solvents

Enzymatic Carbohydrate Hydrolysis Protocol

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Reagents were from Sigma-Aldrich (Milan, Italy), whereas analytical grade solvents were from VWR International (Milan, Italy). Acarbose (from Actinoplanes sp.) and Novozym®435 (Lipase B from Candida antarctica) were obtained from SERVA (Heidelberg, Germany) and Novozymes (Bagsværd, Denmark), respectively.

Leporini M., Loizzo M.R., Tundis R., La Torre C., Fazio A, & Plastina P. (2019). Non-Pungent n-3 Polyunsaturated Fatty Acid (PUFA)-Derived Capsaicin Analogues as Potential Functional Ingredients with Antioxidant and Carbohydrate-Hydrolysing Enzyme Inhibitory Activities. Antioxidants, 8(6), 162.

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Isolation and Characterization of Senna Compounds

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Dried and cut leaflets (Lot 26094) and pods (Lot 22421) were obtained from Alfred Galke GmbH (Bad Grund, Germany). Retard tablets (Sennalax^®, Lot 2014211) were bought in a local pharmacy. SPE cartridges (Chromabond HR-XA, 85 µm, 3 mL/500 mg) and a 70 mL reservoir column were obtained from Macherey-Nagel GmbH (Düren, Germany), and cartridges for flash chromatography (Flashpure Ecoflex Silica SL 50 μm irregular 10 g, Lot 150317B) were ordered from Büchi (Flawil, Switzerland). Dimethyl sulfoxide-d₆ (99.80%, lot S1051, batch 0119E) for NMR spectroscopy was purchased from Eurisotop GmbH, Saarbrücken, Germany, and conventional 5 mm sample tubes were obtained from Rototec-Spintec GmbH, Griesheim, Germany. LCMS-grade acetonitrile, gradient-grade methanol, analytical-grade solvents, and TLC plates (silica gel 60 F254) were purchased from VWR International GmbH, Darmstadt, Germany. Hydrochloric acid (34%) and trifluoro acetic acid (TFA) were obtained from Carl Roth GmbH (Karlsruhe, Germany), acetic acid was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA), and LCMS-grade formic acid was purchased from Merck (Darmstadt, Germany). Water was doubly distilled in-house.

Çiçek S.S., Moreno Cardenas C, & Girreser U. (2022). Determination of Total Sennosides and Sennosides A, B, and A1 in Senna Leaflets, Pods, and Tablets by Two-Dimensional qNMR. Molecules, 27(21), 7349.

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Analytical Solvent Procurement for Research

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Analytical-grade solvents were obtained from VWR International S.r.l., whereas chemicals and reagents were bought from Sigma-Aldrich S.p.a. (Milan, Italy).

Loizzo M.R., Sicari V., Spizzirri U.G., Romeo R., Tundis R., Mincione A., Nicoletta F.P, & Restuccia D. (2022). Evaluation of Selected Quality Parameters of “Agristigna” Monovarietal Extra Virgin Olive Oil and Its Apple Vinegar-Based Dressing during Storage. Foods, 11(8), 1113.

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Ascorbic Acid Purity Determination

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All chemicals and reagents, including a 99.7% purity ascorbic acid standard, were purchased from Sigma-Aldrich S.p.a. (Milan, Italy). Analytical grade solvents were bought from VWR International S.r.l. (Milan, Italy).

Fazio A., La Torre C., Caroleo M.C., Caputo P., Cannataro R., Plastina P, & Cione E. (2020). Effect of Addition of Pectins from Jujubes (Ziziphus jujuba Mill.) on Vitamin C Production during Heterolactic Fermentation. Molecules, 25(11), 2706.

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Reagents and Media Procurement Protocol

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All reagents were purchased from Sigma-Aldrich S.p.a. (Milano, Italy), whereas analytical-grade solvents were obtained from VWR International s.r.l. (Milan, Italy). The microbiological reagent Plate Count Agar medium was purchased from Conda-Pronadisa, (Spain) and the Dichloran Rose-Bengal Chloramphenicol agar medium was obtained from VWR Chemicals s.r.l. (Milan, Italy).

Sicari V., Leporini M., Romeo R., Poiana M., Tundis R, & Loizzo M.R. (2021). Shelf-Life Evaluation of “San Marzano” Dried Tomato Slices Preserved in Extra Virgin Olive Oil. Foods, 10(8), 1706.

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Phytochemical and Cytotoxic Screening of Plant Extracts

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UHPLC-grade n-hexane, acetonitrile, methanol, water, and formic acid Supelco^® were purchased from Merck KGaA (Darmstadt, Germany). All analytical-grade solvents were purchased from VWR (Milano, Italy). Standards of rutin, hesperidin, and gallic acid were purchased from Merck KGaA (Darmstadt, Germany), cyanidin 3-O-glucoside chloride was purchased from Extrasynthese (Extrasynthese, France), and luteolin-4′-O-neohesperidoside was previously obtained in our laboratory by isolation from plant materials and characterised by 1D- and 2D-NMR techniques. Folin–Ciocâlteu reagent was purchased from Merck KGaA (Darmstadt, Germany). The Balb/3T3 clone A31 mouse embryo fibroblast cell line was purchased from the American Type Culture Collection LGC standards (ATCC CCL163, Milan, Italy) and propagated as indicated by the supplier. Dulbecco’s Modified Eagle’s medium (DMEM), 0.01 M pH 7.4 phosphate buffer saline without Ca²⁺ and Mg²⁺ (PBS), bovine calf serum (BCS), glutamine, and antibiotics (penicillin/streptomycin) were obtained from Merck KGaA (Darmstadt, Germany). Cell proliferation reagent WST-1 was provided by Roche Diagnostic (Milan, Italy).

Cioni E., Migone C., Ascrizzi R., Muscatello B., De Leo M., Piras A.M., Zambito Y., Flamini G, & Pistelli L. (2022). Comparing Metabolomic and Essential Oil Fingerprints of Citrus australasica F. Muell (Finger Lime) Varieties and Their In Vitro Antioxidant Activity. Antioxidants, 11(10), 2047.

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Lipid Standards and Solvents Identification

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C 17 -Ceramide was purchased from Avanti-Polar Lipids Inc. (Alabaster, AL, USA). Other lipid standards, as well as fused silica SP 2340 capillary column for chromatography were from Sigma-Aldrich (Taufkirchen, Germany). Analytical grade solvents were obtained from VWR (Bruchsal, Germany). Thin-Layer Chromatography plates (Silica gel G60) and fused silica FFAP capillary column were purchased from Macherey and Nagel (Düren, Germany) and Sigma-Aldrich (Taufkirchen, Germany).

Makdessi S.A., Sweidan H., Schmid E., Weimar U., Gulbins E, & Lang F. (2016). Quantitative Determination of Ceramide Molecular Species in Dendritic Cells. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 39(4).

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