TCT (99%) was purchased from Shanghai McLean Biochemical Technology Co., Ltd.; EDA, tetrahydrofuran (THF, 99%), and potassium dichromate were provided by Chengdu Cologne Chemical Co., Ltd. (Chengdu, China); HAuCl4·4H2O was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China); and other metal salt solutions purchased fromTianjin Fengchuan Chemical Reagent Technology Co., Ltd. (Tianjin, China). All reagents were used as received without further purification. The experimental water was ultrapure water (18.25 MΩ).
Haucl4 4h2o
HAuCl4·4H2O is a chemical compound that consists of gold (Au) and chlorine (Cl) in a hydrated form. It is a yellow, crystalline powder that is commonly used as a precursor in the synthesis of various gold nanoparticles and other gold-based materials.
Lab products found in correlation
25 protocols using haucl4 4h2o
Synthesis and Characterization of Nanomaterials
TCT (99%) was purchased from Shanghai McLean Biochemical Technology Co., Ltd.; EDA, tetrahydrofuran (THF, 99%), and potassium dichromate were provided by Chengdu Cologne Chemical Co., Ltd. (Chengdu, China); HAuCl4·4H2O was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China); and other metal salt solutions purchased fromTianjin Fengchuan Chemical Reagent Technology Co., Ltd. (Tianjin, China). All reagents were used as received without further purification. The experimental water was ultrapure water (18.25 MΩ).
Multifunctional PAMAM Dendrimer Synthesis
Coumarin-Based Gold Nanoparticle Synthesis
OTA Detection Using Aptamer-Based Biosensor
The single-strand DNA (ssDNA) aptamer used in the present study was synthesized by Sangong Biotech Co., Ltd. (Shanghai, China). The sequence of the ochratoxin A (OTA) aptamer was 5′-GAT CGG GTG TGG GTG GCG TAA AGG GAG CAT CGG ACA-3′ (Fig. 1).
Synthesis of Gold Nanoparticles
Synthesis of Gold and Platinum Nanoparticles
Luminol-Based Chemiluminescence Detection Protocol
Electrochemical Immunosensor for IgG Detection
HAuCl4·4H2O was purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). FeCl3∙6H2O was purchased from Damao Chemical Reagent Co., Ltd (Tianjin, China). 3-aminopropyl triethoxysilane (APTES) was purchased from XiBao biological technology co., Ltd. (Shanghai, China). Human immunoglobulin G (IgG), goat Anti-Human IgG and bovine serum albumin (BSA, 96–99%) were obtained from Dingguo Biochemical Reagents (Beijing, China). All other reagents were at analytical grade and ultrapure water was used throughout the study.
Ultrasensitive HCG Detection Assay
gonadotropin (HCG,
Beijing Boosen Biotechnology Co., Ltd.) freeze-dried powder was dissolved
in 1.0 mL of water and then diluted to 10 mL to obtain a 50 μg/mL
HCG standard solution. The solution was diluted to a solution of 1
μg/mL before use. A 0.1 mg/mL rabbit antibody of HCG (RHCG,
Beijing Boosen Biotechnology Co., Ltd.), 84 μmoL/L (1%) HAuCl4·4H2O (Sinopharm Chemical Reagent Co., Ltd.),
0.1 moL/L H2O2, 1% trisodium citrate (TCA, Guangdong
Shantou Xilong Chemical Factory), 0.5 moL/L glucose (GS), 0.1 moL/L
HCl, 0.3 mol/L CH3COOH, and 0.1 mmoL/L Victoria blue 4R
(VB4r) were prepared. Graphene oxide (GO) was prepared by the Hummer
procedure,30 (link) and 1 mg of GO was dissolved
in 100 mL of water by means of ultrasound to obtain a concentration
of 10 μg/mL of GO. AuNPs with size of 8 nm were prepared by
the NaBH4 procedure. All reagents are analytically pure,
and the water was double-distilled.
Synthesis of Gold Nanoparticles
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