Total protein

Manufactured by Biodiagnostic

Sourced in Egypt

The total protein assay is a laboratory test used to measure the total amount of proteins present in a biological sample, such as blood or urine. This test provides a general assessment of the overall protein levels in the body and can be used as a screening tool for various medical conditions.

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Lab products found in correlation

4 protocols using total protein

Hepatoprotective and Antioxidant Evaluation

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Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein, albumin, creatinine, urea, and uric acids were purchased from Biodiagnostics Company (Dokki, Giza, Egypt). The RNA extraction Kit was purchased from Introbio Company, Cat.No. 17063. Reverse transcriptase Polymerase chain reaction (RT-PCR) sensiFAST™ cDNA synthesis kit (Bioline, United Kingdom). All used primers are commercially based. Aflatoxin from the Mycology and Mycotoxins Department, Animal Health Research Institute (ARC), Dokki, Egypt. Curcumin Nanoparticles (NT-Cur-NPs) were purchased from Nanotech Egypt for photo- electronics at the city of 6 October, Al Giza. Saccharomyces cerevisiae cell wall extract (100%, Beta-glucan: 20% minimum &Manan Oligosaccharides: 20% minimum.) was obtained from IBEX Group for animal nutrition and health products and produced by Shandong Bio Sunkeen Co., Ltd (a state-owned high-tech enterprise that integrates R&D, production and sales with rich experience of yeast and nature yeast products.

Ashry A., Taha N.M., Lebda M.A., Abdo W., El-Diasty E.M., Fadl S.E, & Morsi Elkamshishi M. (2022). Ameliorative effect of nanocurcumin and Saccharomyces cell wall alone and in combination against aflatoxicosis in broilers. BMC Veterinary Research, 18, 178.

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Biochemical Evaluation of Cypermethrin Exposure

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The assay kits used for biochemical measurements of aspartate aminotransferases (AST; EC 2.6.1.1.), alanine aminotransferases (ALT; EC 2.6.1.2), alkaline phosphatase (ALP; EC 3.1.3.1), lactate dehydrogenase (LDH; EC 1.1.1.27), catalase (CAT; EC 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1), glutathione-s-transferase (GST; EC 2.5.1.18), glutathione reduced (GSH), malondialdehyde (MDA) and total protein were purchased from Biodiagnostic Company, Dokki, Giza, Egypt. Cypermethrin (95.1 %) was obtained from Jiangsu Yangnong Chemical Co., Ltd, China. All other chemicals used were of analytical reagent grade. All reagents were purchased from Sigma–Aldrich–Fluka (Saint-Quentin France).

Mossa A.T., Heikal T.M., Belaiba M., Raoelison E.G., Ferhout H, & Bouajila J. (2015). Antioxidant activity and hepatoprotective potential of Cedrelopsis grevei on cypermethrin induced oxidative stress and liver damage in male mice. BMC Complementary and Alternative Medicine, 15, 251.

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Biochemical Assays for Oxidative Stress

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All kits used for biochemical studies such as catalase (EC 1.11.1.6), superoxide dismutase (EC 1.15.1.1), glutathione peroxidase (EC 1.11.1.9), glutathione reduced, lipid peroxidation, total protein, uric acid and creatinine were purchased from Biodiagnostic Company, Dokki, Giza, Egypt. All other chemicals were of reagent grades and obtained from Sigma-Aldrich and local scientific distributors in Egypt.

Gad M., Abdel-Rahman H., & Tawfeek E.E. (2020). Gene expression, DNA and kidney damage induced by pirimiphos-methyl in male mice: Chemical composition and ameliorative role of Origanum majorana leaves extract. Egyptian Journal of Chemistry, 0(0), 0-0.

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Biosafety Evaluation of Silver Nanoparticles

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Silver nanoparticles (AgNPs) with average size (15 ± 3 nm) were purchased from Nanotech Egypt for Photo-Electronics (El-Wahaat Road, Dream Land City, Entrance 3, City of 6 October, Giza, Egypt. Vials were diluted by phosphate buffer saline (PBS) and the concentration was adjusted to 0.25 mg/kg, in 200 µl (Hemen Moradi-Sardareha et al., 2018) . Aspartate amino transferase (AST), alanine aminotransferase (ALT), urea, creatinine, albumin, total protein, superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH) and malondialdehyde (MDA) kits were purchased from Bio diagnostic company, Egypt.

El-Naggar S., Ayyad E.k., Khandil R., & Salem M.A. (2021). Phoenix dactylifera seed extract ameliorates toxicity induced in mice by silver nanoparticles through antioxidant effects. International Journal of Cancer and Biomedical Research, 0(0), 0-0.

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