The adipose levels of NF-κB, TNF- α and IL-6 were determined by quantitative standard sandwich ELISA technique using a monoclonal antibody specific for these parameters using rat kits with cat number E-EL-R0674, E-EL-R0019 and E-EL-R0015 respectively obtained from Elabscience Biotechnology Inc. (Wuhan, Hubei, P.R.C., China).
E el r0015
Manufactured by Elabscience
Sourced in China
The E-EL-R0015 is a laboratory equipment designed for use in various scientific applications. It serves as a versatile and reliable tool for researchers and scientists. The core function of this product is to provide a stable and controlled environment for various experiments and analyses.
Automatically generated - may contain errors
8 protocols using e el r0015
1
Quantitative ELISA Analysis of NF-κB, TNF-α, and IL-6
2
Assessment of Serum Inflammatory Markers
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Serum levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were assessed biochemically using rat-specific ELISA kits (Cat. No. E-EL-R2856 and E-EL-R0015, respectively, Elabscience, Wuhan, China). Moreover, the C-reactive protein (CRP) was assessed using an ELISA kit from Elabscience (Cat. No. E-EL-R0506, Elabscience, Wuhan, China) following the guidelines of the manufacturer.
3
Serum Biomarker Analysis in Rats
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After experiment completion, rats of each group were euthanized by cervical decapitation. Blood samples and paw tissues were collected. Blood was collected in a vacuum vessel containing no anticoagulant, centrifuged it at 3000 rpm for 4 min (min), and a serum sample was obtained. Further, serum samples were analyzed by an automatic hemocytometer to examine suppression of biochemical biomarkers C-reactive protein (CRP) and rheumatoid factor (RF). The ELISA (Enzyme-Linked Immunosorbent Assay) was also performed using a kit protocol (Elabscience, E-EL-R0019, and E-EL-R0015 catalog numbers) to calculate tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) concentration in blood serum [49 (link)].
4
Quantifying Inflammatory Cytokines in Serum
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ELISA was performed to determine the concentrations of tumor necrosis factor and interleukin 6 in serum using kit protocols (Elabscience, Catalog Number E-EL-R0019 and E-EL-R0015) (Cheng et al., 2015 (link)).
5
Measuring Serum IL-6 Levels
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The serum level
of IL-6 was measured by using an IL-6 ELISA kit (E-EL-R0015, Elabscience)
through a microplate ELISA reader.
of IL-6 was measured by using an IL-6 ELISA kit (E-EL-R0015, Elabscience)
through a microplate ELISA reader.
6
Measuring Serum IL-6 in Neuroinflamm
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Measuring the serum level of IL-6 in neuroinflammation and cardiometabolic disturbance is essential because IL-6 is a key pro-inflammatory cytokine that plays a crucial role in the immune response and inflammatory processes. Elevated levels of IL-6 can indicate the presence and severity of inflammation, and its measurement helps in understanding the immune and inflammatory status in these conditions. The serum level of IL-6 was measured using an IL-6 ELISA kit (E-ELR0015, Elabscience) with a microplate ELISA reader.
7
Serum Biomarkers of Inflammation and Adiposity
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The serum concentrations
of inflammatory biomarkers, namely, interleukin-6 (IL-6) (Catalog
Number: E-EL-R0015, Elabscience) and tumor necrosis factor-α
(TNF-α) (Catalog Number: E-EL-R0019, Elabscience), as well as
adipokines, specifically, adiponectin (Catalog Number: E-BC-K013-S,
Elabscience) and leptin (Catalog Number: 201905, Elabscience), were
quantified using commercially available ELISA kits. These measurements
were conducted with the aid of microplate ELISA.
of inflammatory biomarkers, namely, interleukin-6 (IL-6) (Catalog
Number: E-EL-R0015, Elabscience) and tumor necrosis factor-α
(TNF-α) (Catalog Number: E-EL-R0019, Elabscience), as well as
adipokines, specifically, adiponectin (Catalog Number: E-BC-K013-S,
Elabscience) and leptin (Catalog Number: 201905, Elabscience), were
quantified using commercially available ELISA kits. These measurements
were conducted with the aid of microplate ELISA.
8
Measuring Serum Cytokine Levels
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ELISA was
performed to measure the serum concentrations of TNF-α
and IL-6 utilizing kit protocols (Elabscience, catalog numbers E-EL-R0019
and E-EL-R0015).51a
performed to measure the serum concentrations of TNF-α
and IL-6 utilizing kit protocols (Elabscience, catalog numbers E-EL-R0019
and E-EL-R0015).51a
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