pCMV-myc-MST1 and kinase-dead (MST1-K59; dnMST1) was kindly provided by Dr. Junichi Sadoshima and Dr. Yasuhiro Maejima (UMDNJ, New Jersey Medical School) 30 (link). Mouse pB.RSV.PDX1-GFP plasmid was kindly provided by Dr. Ingo Leibiger (Karolinska University, Stockholm). pcDNA3 Myr-HA Akt1, HA-Ubiquitin and pCDNA3 Jnk1a1 (apf)(dn-JNK) plasmids were obtained from Addgene (Cambridge, MA). Mouse PDX1 mutants (T11, T126, T152, T155, T214 and T231) in pCGIG5 vector were generated by site-directed mutagenesis as described previously 38 (link). All mutations were verified by sequencing. To make bacterial expression plasmids for PDX1 mutants, the complete mouse PDX1 CDS (wild type and mutants) has been amplified by PCR using a specific set of primers from pCGIG5 plasmids and cloned into a pGEX-6P-1 bacterial expression vector (kindly provided by Dr. Reinhard Walther, University of Greifswald). The rat insulin driven luciferase vector (RIP-Luc) was constructed by subcloning a 700bp fragment containing -660bp of the rat 2 insulin promoter (kindly provided by Dr. Rolf Zinkernagel, University of Zurich) into a pMCS-Green-Renilla-Luc vector (Thermo Scientific). pCMV-Red firefly Luc vector was obtained from Thermo Scientific.
Pmcs green renilla luc vector
Manufactured by Thermo Fisher Scientific
The PMCS-Green-Renilla-Luc vector is a plasmid designed for gene expression studies. It contains a multiple cloning site, a green fluorescent protein (GFP) reporter gene, and a Renilla luciferase reporter gene. The vector allows for the simultaneous monitoring of gene expression using both fluorescence and bioluminescence detection methods.
Automatically generated - may contain errors
2 protocols using pmcs green renilla luc vector
1
Plasmid Constructs for Diabetes Research
2
Plasmid Constructs for Diabetes Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
pCMV-myc-MST1 and kinase-dead (MST1-K59; dnMST1) was kindly provided by Dr. Junichi Sadoshima and Dr. Yasuhiro Maejima (UMDNJ, New Jersey Medical School) 30 (link). Mouse pB.RSV.PDX1-GFP plasmid was kindly provided by Dr. Ingo Leibiger (Karolinska University, Stockholm). pcDNA3 Myr-HA Akt1, HA-Ubiquitin and pCDNA3 Jnk1a1 (apf)(dn-JNK) plasmids were obtained from Addgene (Cambridge, MA). Mouse PDX1 mutants (T11, T126, T152, T155, T214 and T231) in pCGIG5 vector were generated by site-directed mutagenesis as described previously 38 (link). All mutations were verified by sequencing. To make bacterial expression plasmids for PDX1 mutants, the complete mouse PDX1 CDS (wild type and mutants) has been amplified by PCR using a specific set of primers from pCGIG5 plasmids and cloned into a pGEX-6P-1 bacterial expression vector (kindly provided by Dr. Reinhard Walther, University of Greifswald). The rat insulin driven luciferase vector (RIP-Luc) was constructed by subcloning a 700bp fragment containing -660bp of the rat 2 insulin promoter (kindly provided by Dr. Rolf Zinkernagel, University of Zurich) into a pMCS-Green-Renilla-Luc vector (Thermo Scientific). pCMV-Red firefly Luc vector was obtained from Thermo Scientific.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!