Poly u

Manufactured by InvivoGen

Sourced in United States

Poly(U) is a synthetic homopolymer of uridine. It is a laboratory research reagent used in various biochemical and molecular biology applications.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Odn2216, by InvivoGen (1 mentions) Poly 1 c, by InvivoGen (1 mentions) Z ietd fmk, by Enzo Life Sciences (1 mentions) Peptidoglycan, by InvivoGen (1 mentions) Pam3csk4, by Merck Group (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) R848 resiquimod, by Enzo Life Sciences (1 mentions) Tpca 1, by Merck Group (1 mentions) Dotap liposomal transfection reagent, by Roche (1 mentions) Dna loading dye, by Thermo Fisher Scientific (1 mentions) Acrylic acid n hydroxysuccinimide ester, by Merck Group (1 mentions) Agarose multi purpose, by Roche (1 mentions) Dulbecco s phosphate buffered saline dpbs, by Thermo Fisher Scientific (1 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions) Sybr safe dna gel stain, by Thermo Fisher Scientific (1 mentions) Ovalbumin (ova), by InvivoGen (1 mentions)

Spelling variants of this product

Poly(A:U) (9 citations) Polyadenylic-polyuridylic acid (poly A:U) (2 citations)

4 protocols using poly u

pDC Stimulation and Cytokine Assay

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Isolated spleen or BM pDCs (100k) were resuspended in 100 μL of complete 1640 medium and then stimulated in a 96-well U-bottom plate with 1-μM ODN2216 or 10-μg/mL poly U (Invivogen, San Diego, CA, USA) supplemented with Lipofectamine 2000 (ThermoFisher Scientific) (1.5 μL: 1000 μL) for 48 h. The supernatants were collected and stored at −80 °C for enzyme-linked immunosorbent assay (ELISA). Each group consisted of at least 3 mice, and each experiment was repeated at least 3 times.

Zhou Z., Ma J., Xiao C., Han X., Qiu R., Wang Y., Zhou Y., Wu L., Huang X, & Shen N. (2016). Phenotypic and functional alterations of pDCs in lupus-prone mice. Scientific Reports, 6, 20373.

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Investigating HIV Infection and Immune Response in Memory CD4 T Cells

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Most experiments involved culture of uninfected or HIV-infected memory CD4 T cells in IL2 (20ng/ml) medium for up to 1 week, followed by data collection. Cells were cultured in 48-well plates (5x10⁵ cells in 1ml medium) with appropriate reagents. The HIV antiviral inhibitor, Saquinavir, was acquired from NIHAIDS Reagent Program and used at 1–10μg/ml. The GrzB/caspase inhibitor, Z-IETD-FMK (Enzo Life Sciences, Farmingdale, NY USA), was used at 10μM. Experiments involving TLR stimulation of memory CD4 T cells utilized synthetic TLR ligands LPS (Sigma, St. Louis, MO USA), Pam3CSK4 (EMD Millipore, Billerica, MA USA), peptidoglycan, Poly(I:C), and Poly(U) (InvivoGen, San Diego, CA USA), and were used at 2–25μg/ml +/− CD3 activation (clone UCHT1 coated at 0.1μg/ml).

Couturier J., Hutchison A.T., Medina M.A., Gingaras C., Urvil P., Yu X., Nguyen C., Mahale P., Lin L., Kozinetz C.A., Schmitz J.E., Kimata J.T., Savidge T.C, & Lewis D.E. (2014). HIV replication in conjunction with granzyme B production by CCR5+ memory CD4 T cells: implications for bystander cell and tissue pathologies. Virology, 0, 175-188.

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Immunological Response Activation Assay

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Mycophenolic acid, the active moiety of MMF, was purchased from Tokyo Chemical Industry. PSL, HCQ, and TPCA-1 were bought from Sigma-Aldrich. Poly(U) (InvivoGen) was complexed with the DOTAP Liposomal Transfection Reagent (Roche) at a ratio of 1 μg of Poly(U) per 6 μl of DOTAP and was used for stimulation. R-848 (resiquimod) was acquired from Enzo Life Sciences. CpG-B ODN 1668 (5′-T*C*C*A*T*G*A*C*G*T*T*C*C*T*G*A*T*G*C*T-3′) and CpG-A ODN 2216 (5′-G*G*GGGACGATCGTCG*G*G*G*G*G-3′) were synthesized by Eurofins Genomics (an asterisk indicates a phosphorothioate linkage). The monoclonal antibody that specifically recognizes phosphorylated IRF5 (anti-phospho-IRF5 antibody) was generated by the antibody phage display method^{53 (link)} following the immunization of rabbits with a synthetic peptide corresponding to the region around phosphorylated serine 446 (S446) of human IRF5 isoform B (equivalent to the S462 of human IRF5 isoform D).

Ban T., Kikuchi M., Sato G.R., Manabe A., Tagata N., Harita K., Nishiyama A., Nishimura K., Yoshimi R., Kirino Y., Yanai H., Matsumoto Y., Suzuki S., Hihara H., Ito M., Tsukahara K., Yoshimatsu K., Yamamoto T., Taniguchi T., Nakajima H., Ito S, & Tamura T. (2021). Genetic and chemical inhibition of IRF5 suppresses pre-existing mouse lupus-like disease. Nature Communications, 12, 4379.

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Synthesis and Characterization of Novel Polymeric Agents

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Imidazole-1-sulfonyl azide hydrochloride [25] , N-(2-hydroxyl propyl)-methacrylamid (HPMA) and DMAE-CI [26] were synthesized as previously described. Azobis(isobutyronitrile) (AIBN), 4-cyano-4-[(dodecylsulfanylthiocarbonyl)sulfanyl]pentanoic acid (CDTPA), azidoacetic acid N-hydroxysuccinimide ester, acrylic acid N-hydroxysuccinimide ester (NAS) and all other reagents and solvents used were obtained from Sigma (Zwijndrecht, the Netherlands) and were used without further purification. 2,5-Dioxopyrrolidin-1-yl 4-((((1R,8S,9s)-bicyclo [6.1.0] non-4-yn-9-ylmethoxy)carbonyl)amino) butanoate (BCN, SX-A1036) was purchased from Synaffix BV (Oss, the Netherlands). Ovalbumin (OVA) and PolyU were purchased from In-vivoGen (Toulouse, France). Cy5-mRNA_luc was from Teu-bio (TRiLink biotechnologies, San Diego, USA). Agarose multi-purpose was purchased from Roche Molecular Biochemicals (Mannheim, Germany). 6× DNA Loading Dye was purchased from Fermentas (St. Leon-Roth, Germany). SYBR Safe DNA gel stain, Dulbecco's phosphate buffered saline (DPBS), RPMI-1640/IMDM medium, Opti-MEM, and heat inactivated fetal bovine serum (HI-FBS) were purchased from Life Technologies (Breda, the Netherlands).

Lou B., De Beuckelaer A., Boonstra E., Li D., De Geest B.G., De Koker S., Mastrobattista E, & Hennink W.E. (2019). Modular core-shell polymeric nanoparticles mimicking viral structures for vaccination. Journal of controlled release : official journal of the Controlled Release Society, 293.

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