Whole-mount mRNA in situ hybridization (WMISH) was carried out as previously reported [42 (link)]. Three-color fluorescent in situ hybridization was performed as described previously [43 (link)]. Images shown are representative of three independent hybridization experiments. Multicolor WMISH contained a SpGalnt7-2 probe labeled with digoxigenin and detected with Cy5-TSA, a Gcm probe labeled with dNP and detected with fluorescein-TSA, and a Endo16 probe labeled with fluorescein and detected with Cy3-TSA. WMISH panel images were taken with a Nikon Eclipse Ti-E inverted microscope with a DS-Ri2 color CMOS camera at 20X. Nikon NIS Elements was used to acquire and crop images. Triply labeled images were taken with a Zeiss Axiovert 200M microscope, a 25x objective (Zeiss) and the Apotome system. Optical sections were stacked and cropped with Adobe Photoshop.
Ds ri2 color cmos camera
Manufactured by Nikon
The DS-Ri2 is a color CMOS camera designed for microscopy applications. It features a high-resolution image sensor and supports a variety of image capture modes. The camera is compatible with Nikon's digital microscope systems.
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Lab products found in correlation
2 protocols using ds ri2 color cmos camera
1
Fluorescent Whole-Mount In Situ Hybridization
2
Quantification of Cell Proliferation and Apoptosis
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After staining, sections were visualized with an Eclipse Ti2 microscope (Nikon Instruments Inc., Melville, NY). Representative images were obtained with a Nikon DS-Qi2 monochrome CMOS camera or a DS-Ri2 color CMOS camera using Nikon NIS-Elements imaging software version 5.2. Cells positive for BrdU, Ki-67, and TUNEL were quantified with digital images from 3 to 5 random microscopic fields per tissue.
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