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5 protocols using dodecanthiol

1

Synthesis and Characterization of Magnetic Nanoparticles

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Methacrylic acid (MAA, 99%), 2,2′-azobisisobutyronitrile (AIBN, 98%), anhydrous ethanol, Ferric chloride (FeCl3·6H2O, 99%), ferrous sulfate (FeSO4·7H2O, 99%), hydrochloric acid (HCl, 38%), ammonium hydroxide (NH4OH, 28%), anhydrous acetone, and anhydrous diethyl ether were purchased from National Medicines Corporation Ltd. of P. R. China (Beijing, China). Dodecanthiol (DDT, 99%) was purchased from Sigma-Aldrich (Shanghai, China). RPMI Medium 1640, Dulbecco’s modified eagle’s medium (DMEM), and phosphate buffer saline (PBS) were purchased from HyClone (Logan, UT, USA). Fetal bovine serum (FBS) was purchased from Gibco (Basel, Switzerland). Trypsin-EDTA (0.25%) phenol red, Penicillin-Streptomycin Solution, Cell Counting Kit (CCK-8) were purchased from Yeasen (Shang Hai, China).
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2

Synthesis of Gold Nanoparticles

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Hydrogen tetrachloroaurate hydrate, 99.999%, (trace metal basis) Acros Organics™ was purchased from Fisher Scientific Italia (Rodano, Italy). Meo-PEG-SH (2000 Da) was purchased from RappPolymere GmbH (Tuebingen, Germany). Boric acid, CHCl3, dodecylamine (99%), dodecanthiol, HCl, MeOH, NaCl, NaOH, pentane, poly(isobutylene-alt-maleic anhydride) (molecular weight (Mw) ~6000), sodium borohydride (≥99%), sodium citrate dihydrate (99%), tetrahydrofuran, tetra-n-octylammonium bromide (98%), and toluene were purchased from Sigma Aldrich (Saint Louis, MO, USA).
Water used in all procedures was purified by passing through a MilliQ Millipore system.
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3

Synthesis of Colloidal Metal Nanoparticles

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Manganese(II) chloride (reagent grade, ≥99%), tetramethylammonium hydroxide pentahydrate, sulfur powder (99.98%), dimanganese decacarbonyl (98%), oleic acid (90%), stearic acid (99%), oleylamine (technical grade, 70%), dodecylamine (98%), hexadecylamine (90%), octadecylamine (≥99%), dodecanthiol (98%), oleylalcohol (≥85%), tryoctylphosphine (97%), and octadecene (purum, ≥95.0%) were purchased from Sigma-Aldrich. stearic acid (99%) was purchased from Calbiochem. All chemicals were used as received without further purification.
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4

Synthesis and Aging of In2S3 Quantum Dots

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Indium Chloride (99.9%, Sigma Aldrich), Elemental Sulfur (99.9%, Sigma Aldrich) were used as a source material for In3+ and S2− ions respectively. Dodecanthiol (99%, Sigma Aldrich) and Oleylamine (98%, Aldrich) were used for the capping purpose. Acetone (99%, Loba Chemicals) was used for aging study. A 0.001 mol of Indium Chloride was dissolved in 1-Dodecanthiol and stirred under Argon atmosphere at 110 °C for half an hour. Elemental Sulfur was dissolved in Oleylamine and stirred under vacuum at 90 °C for half an hour. After that Elemental Sulfur solution was quickly injected into Indium Chloride solution and mixed under Argon atmosphere for 20 min to grow indium sulfide quantum dots. As obtained quantum dots were purified by means of using equimolar ratio of Hexane (Solvent) and Methanol (Antisolvent) under centrifugation at 5000 rpm for 30 min. As prepared Oleylamine and 1-Dodecanthiol capped blue emitting In2S3 quantum dots were dissolved in Acetone and kept under aging for 1 month.
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5

Cardiac Biomarker Immunoassay Development

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Hydrogen tetrachloroaurate trihydrate (HAuCl4; 99%), sodium borohydride (NaBH4; 99%), Cetyltrimethylammoniumbromide (CTAB), L-ascorbic acid (AA), silver nitrate (AgNO3; 99%), dodecanthiol (DDT), 11-mercaptoundecanoic acid (MUDA; >95%), N-hydroxysulfosuccinimide (Sulfo-NHS), 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimidehydrochloride (EDC), human plasma, and fibrinogen were obtained from Sigma-Aldrich (St. Louis, MO). Highly purified human cardiac troponin I (cTnI), myoglobin (MG), and their respective murine monoclonal antibodies were from Fitzgerald Industries (Acton, MA).
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