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7 amino actinomycin 7 aad apoptosis detection kit

Manufactured by BD
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Sourced in Belgium

The 7-amino-actinomycin (7-AAD) apoptosis detection kit is a laboratory tool used to detect apoptosis, a form of programmed cell death. The kit provides a reliable and objective method for quantifying apoptotic cells through the binding of 7-AAD, a fluorescent DNA-binding dye, to the DNA of cells undergoing apoptosis.

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Lab products found in correlation

Version 7, by FlowJo (1 mentions) Annexin 5 phycoerythrin pe, by BD (1 mentions) Accuri c6 plus flow cytometer, by BD (1 mentions) Colorimetric tunel apoptosis assay kit, by Beyotime (1 mentions)

Close spelling variants of this product

7-AAD (1194 citations) Apoptosis detection kit (367 citations) 7-amino-actinomycin (7-AAD) (65 citations) 7-amino-actinomycin (25 citations) 7-amino-actinomycin D (7-AAD) apoptosis detection kit (5 citations) Apoptosis kits (4 citations) Annexin V/7-amino-actinomycin D (7-AAD) apoptosis detection kit (4 citations) Annexin V-PE/7-Amino-Actinomycin (7-AAD) Apoptosis Detection Kit (3 citations) PE-AnnexinV and 7-amino-actinomycin D (7-AAD) Apoptosis Detection Kit I (3 citations) PE-Annexin V / 7-amino-actinomycin D (7-AAD) apoptosis detection kit (2 citations)

2 protocols using 7 amino actinomycin 7 aad apoptosis detection kit

1

Apoptosis Analysis of AZD1775 and Cisplatin

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Cells exposed to AZD1775 with/without cisplatin for 48 h were collected, washed in phosphate-buffered saline (PBS), and double-stained using Annexin V-Phycoerythrin (PE) and 7-amino-actinomycin (7-AAD) apoptosis detection kit (BD Biosciences, Erembodegem, Belgium) following the vendor's protocol. Samples were detected by flow cytometry within 1 h (BD Biosciences) and proportions of apoptotic cells were analyzed using the FlowJo version 7.6.1 software (FlowJo, Oregon).
Chen D., Lin X., Gao J., Shen L., Li Z., Dong B., Zhang C, & Zhang X. (2018). Wee1 Inhibitor AZD1775 Combined with Cisplatin Potentiates Anticancer Activity against Gastric Cancer by Increasing DNA Damage and Cell Apoptosis. BioMed Research International, 2018, 5813292.
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2

Apoptosis Analysis of miPS-CSCs Cells

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In vitro cell apoptosis was analyzed by flow cytometry (AccuriTM C6 Plus flow cytometer, BD Biosciences) using a Annexin V-Phycoerythrin (PE) and 7-amino-actinomycin (7-AAD) Apoptosis Detection Kit (BD Biosciences, 559763) after three kinds of miPS-CSCs cells treated with inhibitors for 48 h, we analyzed proportions of apoptotic cells using FlowJo Software (Treestar Inc., San Carlos, CA), whereas in vivo apoptotic tumor cells were detected using a Colorimetric TUNEL Apoptosis Assay Kit (Beyotime, C1091).
Xu Y., Afify S.M., Du J., Liu B., Hassan G., Wang Q., Li H., Liu Y., Fu X., Zhu Z., Chen L, & Seno M. (2022). The efficacy of PI3Kγ and EGFR inhibitors on the suppression of the characteristics of cancer stem cells. Scientific Reports, 12, 347.
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