Goat anti mouse igg3

Manufactured by Southern Biotech

Sourced in United States

Goat anti-mouse IgG3 is a secondary antibody that binds to the IgG3 subclass of mouse immunoglobulins. It is used in various immunological techniques, such as ELISA, Western blotting, and immunohistochemistry, to detect and quantify the presence of mouse IgG3 in samples.

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Anti-IgG3 (8 citations) Anti-mouse IgG3 (7 citations) Goat anti-mouse IgG/IgG1/IgG2a/IgG2b/IgG3 antibodies labeled with Horseradish peroxidase (3 citations) Goat anti-mouse IgG3-HRP (3 citations)

2 protocols using goat anti mouse igg3

Mouse IgG Subclass ELISA

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Plates were coated with mouse IgE F127, IgE(PNG), or Fel d 1. Serial dilution of sera (1:25, then serially 1:3) was added. HRP-labeled goat anti-mouse IgG (The Jackson Laboratory) antibodies were used to detect IgG (1:2’000). To detect IgG subclasses, HRP labeled rat anti-mouse IgG1 (BD Pharmingen), goat anti-mouse IgG2a (Bio-Rad), goat anti-mouse IgG2b (Invitrogen, Carlsbad, CA, USA), and goat anti-mouse IgG3 (SouthernBiotech, Birmingham, AL, USA) were used in a 1:2’000 dilution.

Plattner K., Gharailoo Z., Zinkhan S., Engeroff P., Bachmann M.F, & Vogel M. (2022). IgE glycans promote anti-IgE IgG autoantibodies that facilitate IgE serum clearance via Fc Receptors. Frontiers in Immunology, 13, 1069100.

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Serum Antibody Measurement Protocol

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96-well microplate was coated with goat anti-mouse IgM (Jackson ImmunoResearch) or goat anti-mouse Ig (Southern Biotech) and blocked with 2% BSA. Mouse sera was added in 2-fold serial dilutions and incubated for 1 h at room temperature. Detection was achieved with biotinylated rat anti-mouse IgM (Biolegend, RMM-1), goat anti-mouse IgA, goat anti-mouse IgG1, goat anti-mouse IgG2b, goat anti-mouse IgG2c, goat anti-mouse IgG3, goat anti-mouse Kappa/Lambda (all Southern Biotech) or H-2D(d) monomer (folded with RGPGRAFVTI peptide, NIH Tetramer Facility). Biotinylated antibodies were then detected with streptavidin-horseradish peroxidase reagent (BD Biosciences), followed by development with TMB substrate. The developing reaction was stopped with 1M phosphoric acid and read at 450 nM.
Human protective antibodies were measured using the following kits according to the manufacturer’s instructions: anti-measles IgG (Serion Immunodiagnostica GmbH), anti-mumps IgG (Calbiotech), anti-rubella IgG (Phoenix Pharmaceuticals Inc), anti-tetanus toxoid IgG (Serion Immunodiagnostica GmbH). Total IgM, IgA, and IgG were measured by nephelometry by the clinical laboratory at the Hospital of the University of Pennsylvania.

Zhang Z., Markmann C., Yu M., Agarwal D., Rostami S., Wang W., Liu C., Zhao H., Ochoa T., Parvathaneni K., Xu X., Li E., Gonzalez V., Khadka R., Hoffmann J., Knox J.J., Scholler J., Marcellus B., Allman D., Fraietta J.A., Samelson-Jones B., Milone M.C., Monos D., Garfall A.L., Naji A, & Bhoj V.G. (2023). Immunotherapy targeting B cells and long-lived plasma cells effectively eliminates pre-existing donor-specific allo-antibodies. Cell Reports Medicine, 4(12), 101336.

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