Pcdna3.1 neo expression vector

Manufactured by Thermo Fisher Scientific

The PCDNA3.1-Neo(+) expression vector is a plasmid designed for the expression of recombinant proteins in mammalian cell lines. It contains a multiple cloning site for the insertion of the gene of interest, as well as a neomycin resistance gene for selection of transfected cells.

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Lab products found in correlation

Quikchange 2 xl, by Agilent Technologies (1 mentions)

Close spelling variants of this product

PcDNA3.1 expression vector PcDNA3.1(+) (pcDNA3.1) expression vector PcDNA3 expression vector PcDNA3.1(+)neo PcDNA3.1 vector (vector) PcDNA3.1 vector PcDNA3 vector Expression vectors PcDNA3.1 (þ) PcDNA3.1

2 protocols using pcdna3.1 neo expression vector

Transient Overexpression of GSTM1, MX2, and MxB

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For transient overexpression experiments, full-length GSTM1 and MX2 cDNAs plus the cDNA fragment encoding the short isoform of MxB (MxB Δ1–25, base pairs 76–2148 of MX2 cDNA) were cloned into pCDNA3.1-Neo(+) expression vector (Invitrogen) using the NotI and XbaI restriction sites. The MX2(G51Q) and MX2(T151A) mutant vectors were generated by site-directed mutagenesis (QuikChange II XL, Agilent Technologies) according to the manufacturer’s instructions. The herpesvirus-specific luciferase reporter plasmid pGL-T9G has previously been described^{60 (link)}.

Crameri M., Bauer M., Caduff N., Walker R., Steiner F., Franzoso F.D., Gujer C., Boucke K., Kucera T., Zbinden A., Münz C., Fraefel C., Greber U.F, & Pavlovic J. (2018). MxB is an interferon-induced restriction factor of human herpesviruses. Nature Communications, 9, 1980.

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Engineered Fusion Proteins for Cell Targeting

Cited 1 time

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Fusion constructs were cloned through overlap extension PCR (32 (link)) using wild type human CD137 (UniProt: Q07011; NCBI ref: NM_001561), human CD52 (Uniprot: P31358; NCBI ref: NM_001803.2) or the Rp3/Cp11 epitope (kind gift of Prof Martin Pule and Dr Brian Philip, UCL (33 (link), 34 (link))). Each fusion protein consisted of the CD52 leader sequence, Rp3/Cp11, CD137 (with domains removed based on the annotations provided with Uniprot reference sequence). These gene constructs were cloned into pcDNA3.1/- (neo) expression vector (Invitrogen).

Cleary K.L., Chan H.T., James S., Glennie M.J, & Cragg M.S. (2017). Antibody distance from the cell membrane regulates antibody effector mechanisms. Journal of immunology (Baltimore, Md. : 1950), 198(10), 3999-4011.

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