α-Asarone (trans-1-Propenyl-2,4,5-trimethoxybenzene, Fig. 1 ) and lipopolysaccharide (from Escherichia coli O55:B5) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mouse anti-TNF-α antibody, goat anti-rabbit and goat anti-mouse IgG horseradish peroxidase (HRP) conjugated secondary antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse anti-amyloid precursor protein (APP) antibody was purchased from Millipore (Billerica, MA, USA). Rabbit anti-IL-1β and rabbit anti-β-site APP-converting enzyme 1 (BACE1) antibodies were purchased from Abcam (Cambridge, UK). Rabbit anti-ionized calcium binding adaptor molecule 1 (Iba1) antibodies (#016-20001, #019-19741) were purchased from Wako Pure Chemical Industries (Osaka, Japan). Mouse anti-actin antibody was purchased from Chemicon International (Temecula, CA, USA). The other chemicals and reagents used were of high quality and obtained from various commercial sources.
Goat anti mouse igg horseradish peroxidase hrp conjugated secondary antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Goat anti-mouse IgG horseradish peroxidase (HRP)-conjugated secondary antibody is a laboratory reagent used in immunoassays and immunohistochemistry. It is a secondary antibody that binds to mouse primary antibodies, with HRP enzyme conjugated to it for signal amplification and detection.
Automatically generated - may contain errors
Lab products found in correlation
Mirna mimics and inhibitor, by GenePharma (2 mentions)
P53 antibody, by Beyotime (2 mentions)
Sybr green polymerase chain reaction kit, by Selleck Chemicals (2 mentions)
Triton x, by Vetec (2 mentions)
Dualluciferase reporter vector plasmid, by GenePharma (2 mentions)
Dual luciferase reporter gene uorescence detection kit, by Promega (2 mentions)
3 4 5dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt kit, by Beyotime (2 mentions)
Dulbecco s modi ed eagle medium dmem f12, by Thermo Fisher Scientific (2 mentions)
Revertaid first strand complementary dna cdna synthesis kit, by Thermo Fisher Scientific (2 mentions)
Lipofectamine 3000 transfection reagent, by Thermo Fisher Scientific (2 mentions)
Trizol, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Sartorius (2 mentions)
Glyceraldehyde 3 phosphate dehydrogenase gapdh antibody, by Signalway Antibody (2 mentions)
Crystal violet, by Beyotime (2 mentions)
Cell counting kit 8 (cck8), by Beyotime (2 mentions)
Trypsin, by Beyotime (2 mentions)
Mouse anti tnfα antibody, by Santa Cruz Biotechnology (1 mentions)
α asarone, by Merck Group (1 mentions)
Mouse anti actin antibody, by Merck Group (1 mentions)
Lipopolysaccharide from escherichia coli o55 b5, by Merck Group (1 mentions)
3 protocols using goat anti mouse igg horseradish peroxidase hrp conjugated secondary antibody
1
Neuroinflammation Modulation by α-Asarone
2
Evaluating miRNA Regulatory Mechanisms
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PTX was purchased from Aladdin. Dulbecco's Modi ed Eagle Medium (DMEM)/F12 was purchased from Gibco. Fetal bovine serum (FBS) was purchased from Biological Industries. Trypsin, crystal violet, 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) kit, Cell Counting Kit-8, and P53 antibody were purchased from Beyotime Biotechnology. Trizol was purchased from Ambion. RevertAid First Strand complementary DNA (cDNA) Synthesis Kit was purchased from Thermo. SYBR Green Polymerase Chain Reaction (PCR) kit was purchased from Bimake. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was purchased from Signalway Antibody. Goat anti-mouse IgG horseradish peroxidase (HRP)conjugated secondary antibody was purchased from Santa Cruz Biotechnology. MDM2 antibody was purchased from A nity. Polyvinylidene di uoride (PVDF) membrane was purchased from Immobilon. 0.3% triton-X was purchased from Vetec. Lipofectamine®3000 transfection reagent was purchased from Invitrogen. MiRNA mimics and inhibitors were synthesized by GenePharma (Shanghai, China). Dualluciferase reporter vector plasmid was purchased by GenePharma (Shanghai, China). Dual-luciferase reporter gene uorescence detection kit was purchased by Promega.
3
Evaluating miRNA Regulatory Mechanisms
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PTX was purchased from Aladdin. Dulbecco's Modi ed Eagle Medium (DMEM)/F12 was purchased from Gibco. Fetal bovine serum (FBS) was purchased from Biological Industries. Trypsin, crystal violet, 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) kit, Cell Counting Kit-8, and P53 antibody were purchased from Beyotime Biotechnology. Trizol was purchased from Ambion. RevertAid First Strand complementary DNA (cDNA) Synthesis Kit was purchased from Thermo. SYBR Green Polymerase Chain Reaction (PCR) kit was purchased from Bimake. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was purchased from Signalway Antibody. Goat anti-mouse IgG horseradish peroxidase (HRP)conjugated secondary antibody was purchased from Santa Cruz Biotechnology. MDM2 antibody was purchased from A nity. Polyvinylidene di uoride (PVDF) membrane was purchased from Immobilon. 0.3% triton-X was purchased from Vetec. Lipofectamine®3000 transfection reagent was purchased from Invitrogen. MiRNA mimics and inhibitors were synthesized by GenePharma (Shanghai, China). Dualluciferase reporter vector plasmid was purchased by GenePharma (Shanghai, China). Dual-luciferase reporter gene uorescence detection kit was purchased by Promega.
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