Cd15 mma

The biopsied materials were fixed in 10% neutralbuffered formalin, embedded in paraffin, cut into 4-mm-thick sections, and stained with hematoxylin and eosin for routine histopathological evaluation. All specimens were diagnosed by 2 pathologists (AMM and HT) according to the World Health Organization Classification of Tumours of Haematopoietic and Lymphoid Tissues published in 2008. 12 Immunohistochemical analysis of formalin-fixed, paraffinembedded tissues was performed by standard procedures. Antibodies against the following antigens were used: CD3 (PS1, 1:25; Novocastra, Newcastle, UK), CD5 (4C7, 1:100; Novocastra), CD10 (56C6, 1:100; Novocastra), CD15 (MMA, 1:100; Becton Dickinson, Tokyo, Japan), CD20 (L26, 1:200; Dako, Glostrup, Denmark), CD23 (1B12, 1:100; Novocastra), CD30 (Ber-H2, 1:100; Dako), CD56 (1B6, 1: 100; Novocastra), CD138 (MI15, ready for use; Leica, Newcastle, UK), ALK (ALK1, 1:200; Dako), Bcl-2 (124, 1: 100; Dako), cyclin D1 (SP4, ready for use; Nichirei, Tokyo, Japan), granzyme B (GrB-7, 1:200; Dako), TIA-1 (26gA10F5, 1:1,000; Immunotech, Marseille, France), TdT (poly, 1:20; Dako), k (poly, 1:20,000; Dako), and l (poly, 1: 40,000; Dako). In situ hybridization with an EBV-encoded small nuclear early region (EBER)-1 probe (Dako) was performed to detect possible EBV infection.