Equal amounts of protein (30 μg) were separated by electrophoresis in precast 4-12% Bis-Tris Gels (Bio-Rad) and transferred to activated/pre-wetted PVDF membranes. The membranes were hybridized with the following primary antibodies as indicated: AT8 anti-P-tau pSer202/Thr205 (1:500, Thermo Scientific, #MN1020); anti-P-tau pS396 (1:1000, Abcam, #ab109390); anti-total tau T46 (1:1000, Thermo Scientific, #13-6400); anti-GAPDH (1:2000, Santa Cruz, #sc-32233); anti=P-CaMKII (1:1000, Abcam, #ab32678); anti-PSD-95 (1:1000, Millipore, #7E3-1B8), C1q (1:1000, Abcam, #ab182451). Secondary antibodies included: peroxidase labeled anti-mouse IgG (1:2000, Vector Laboratories); peroxidase labeled anti-rabbit IgG (1:2000, Vector Laboratories); peroxidase labeled anti-rat IgG (1/2000, Vector Laboratories). ECL (Pierce®) was used to reveal the immunoreactive proteins, and images were acquired using a Fujifilm ImageReader LAS-4000. Membranes were stripped using a stripping buffer (Thermo Scientific) when required. Luminescent immunoreactive protein bands were quantified using Fiji software (ImageJ).
Anti total tau t46
Manufactured by Thermo Fisher Scientific
The Anti-total tau T46 is a laboratory reagent produced by Thermo Fisher Scientific. It is used to detect and quantify the total tau protein in biological samples. The core function of this product is to facilitate the analysis and measurement of tau proteins, which are important biomarkers for various neurological conditions.
Automatically generated - may contain errors
Lab products found in correlation
Stripping buffer, by Thermo Fisher Scientific (2 mentions)
Image reader las 4000, by Fujifilm (2 mentions)
Anti gapdh, by Santa Cruz Biotechnology (2 mentions)
Bis tris gel, by Bio-Rad (2 mentions)
Anti psd95, by Merck Group (2 mentions)
Anti p camkii, by Abcam (2 mentions)
Peroxidase labeled anti rabbit igg, by Vector Laboratories (2 mentions)
Anti p tau ps396, by Abcam (2 mentions)
Peroxidase labeled anti mouse igg, by Vector Laboratories (2 mentions)
Peroxidase labeled anti rat igg, by Vector Laboratories (2 mentions)
At8 anti p tau pser202 thr205, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti total tau t46
1
Quantitative Protein Analysis in Tau Pathology
2
Western Blot Analysis of Tau and Synaptic Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Equal amounts of protein (30 μg) were separated by electrophoresis in precast 4–12% Bis-Tris Gels (Bio-Rad) and transferred to activated/pre-wetted polyvinylidene difluoride membranes. The membranes were hybridized with the following primary antibodies as indicated: AT8 anti-P-tau pSer202/Thr205 (1:500, Thermo Scientific, #MN1020); anti-P-tau pS396 (1:1000, Abcam, #ab109390); anti-total tau T46 (1:1000, Thermo Scientific, #13-6400); anti-GAPDH (1:2000, Santa Cruz, #sc-32233); anti-P-CaMKII (1:1000, Abcam, #ab32678); anti-PSD-95 (1:1000, Millipore, #7E3-1B8), C1q (1:1000, Abcam, #ab182451). Secondary antibodies included: peroxidase-labeled anti-mouse IgG (1:2000, Vector Laboratories); peroxidase-labeled anti-rabbit IgG (1:2000, Vector Laboratories); peroxidase-labeled anti-rat IgG (1:2000, Vector Laboratories). ECL (Pierce®) was used to reveal the immunoreactive proteins, and images were acquired using a Fujifilm ImageReader LAS-4000. Membranes were stripped using a stripping buffer (Thermo Scientific) when required. Luminescent immunoreactive protein bands were quantified using Fiji software (ImageJ).
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