Mouse mammary cancer cell line E0771 was provided by Dr. Barbara Fingleton (Vanderbilt University) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Corning #10-013-CV) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. MMTV-PyMT cells were first isolated (18 (link)) and provided by Dr. Rebecca Cook (Vanderbilt University) and were cultured in the same condition as above. All tumor cells were maintained at low passages after thaw and cells identities were confirmed by morphology, growth rate, cell signaling, in comparison with phenotypes described in the literature(15 ,19 (link)). Mycoplasma testing was performed on cultured cells every 6 months, most recently in March 2022, using the MycoStrip-Mycoplasma Detection kit (rep-mys-10, Invitrogen).
Mycostrip mycoplasma detection kit rep mys 10
Manufactured by Thermo Fisher Scientific
The MycoStrip-Mycoplasma Detection kit (rep-mys-10) is a rapid and reliable tool for the detection of mycoplasma contamination in cell cultures. The kit utilizes a strip-based format to perform mycoplasma testing.
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Lab products found in correlation
2 protocols using mycostrip mycoplasma detection kit rep mys 10
1
Characterization of Mouse Mammary Cancer Cell Lines
2
Culturing Mouse Mammary Cancer Cells
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Mouse mammary cancer cell line E0771 was provided by Dr. Barbara Fingleton (Vanderbilt University, Nashville, TN) and cultured in DMEM (Corning #10-013-CV) supplemented with 10% FBS and 100 U/ml penicillin and streptomycin. Mouse mammary tumor virus–driven polyoma virus middle T antigen (MMTV-PyMT) cells were first isolated (18 (link)) and provided by Dr. Rebecca Cook (Vanderbilt University, Nashville, TN) and were cultured in the same condition as above. All tumor cells were maintained at low passages after thaw and cells identities were confirmed by morphology, growth rate, cell signaling, in comparison with phenotypes described in the literature (15, 19 (link)). Mycoplasma testing was performed on cultured cells every 6 months, most recently in March 2022, using the MycoStrip-Mycoplasma Detection kit (rep-mys-10, Invitrogen).
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