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Triple quadrupole 5500

Manufactured by AB Sciex
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The AB Sciex Triple Quadrupole 5500 is a mass spectrometry instrument that utilizes triple quadrupole technology to perform sensitive and selective quantitative analysis. It is designed to provide reliable and accurate results for a wide range of applications.

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Lab products found in correlation

Cobas 6000, by Roche (2 mentions) Qtrap 6500, by AB Sciex (2 mentions) 1100 hplc system, by Agilent Technologies (1 mentions) 1200 hplc system, by Agilent Technologies (1 mentions) Api 4000, by AB Sciex (1 mentions) Automatic analyzer 7600 210, by Hitachi (1 mentions) Api 3000, by Thermo Fisher Scientific (1 mentions) Api 5500, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

QTRAP 5500 MS/MS hybrid system triple quadrupole/linear ion trap mass spectrometer 5500 QTRAP triple quadrupole mass spectrometer 5500 QTrap triple-quadrupole linear ion trap mass spectrometer 5500 triple quadrupole mass spectrometer 5500 QTrap triple-quadrupole linear ion API 5500 triple quadrupole 5500 QTRAP® instrument triple quadrupole mass spectrometer 5500 QTrap triple quadrupole MS/MS system Sciex 5500 QTRAP triple quadrupole mass spectrometer API 5500 QTRAP triple quadrupole mass spectrometer

6 protocols using triple quadrupole 5500

1

Sensitive Quantification of EOAs in Biological Matrices

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A significant number of EOAs are present in pg/mL to ng/mL concentrations in biological matrices. Therefore, we compared the sensitivity of the LC-QTOF/MS method to that of LC-MS/MS, the current gold standard for quantitative analysis of most environmental chemicals. Specifically, we spiked known concentration ranges (0.001 – 100 ng/mL) of bisphenol A-d16, bisphenol A, methyl paraben, monobutyl phthalate, perfluorooctanoic acid, and 4-amino-2-nitrophenol into double charcoal stripped, drug-free serum and injected these samples into the LC-QTOF/MS. We assessed signals obtained for each of the six compounds and we established the LOD of each compound as the lowest concentration of the compound that has a signal-to-noise ratio of ≥ 3. We then compared these LODs to those obtained using the AB Sciex Triple Quadrupole 5500, one of the most sensitive LC-MS/MS platforms at the time we conducted this analysis.
Gerona R.R., Schwartz J.M., Pan J., Friesen M.M., Lin T, & Woodruff T.J. (2017). Suspect Screening of Maternal Serum to Identify New Environmental Chemical Biomonitoring Targets using Liquid Chromatography-Quadrupole Time-Of-Flight Mass Spectrometry. Journal of exposure science & environmental epidemiology, 28(2), 101-108.
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2

Sensitive Quantification of EOAs in Biological Matrices

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A significant number of EOAs are present in pg/mL to ng/mL concentrations in biological matrices. Therefore, we compared the sensitivity of the LC-QTOF/MS method to that of LC-MS/MS, the current gold standard for quantitative analysis of most environmental chemicals. Specifically, we spiked known concentration ranges (0.001 – 100 ng/mL) of bisphenol A-d16, bisphenol A, methyl paraben, monobutyl phthalate, perfluorooctanoic acid, and 4-amino-2-nitrophenol into double charcoal stripped, drug-free serum and injected these samples into the LC-QTOF/MS. We assessed signals obtained for each of the six compounds and we established the LOD of each compound as the lowest concentration of the compound that has a signal-to-noise ratio of ≥ 3. We then compared these LODs to those obtained using the AB Sciex Triple Quadrupole 5500, one of the most sensitive LC-MS/MS platforms at the time we conducted this analysis.
Gerona R.R., Schwartz J.M., Pan J., Friesen M.M., Lin T, & Woodruff T.J. (2017). Suspect Screening of Maternal Serum to Identify New Environmental Chemical Biomonitoring Targets using Liquid Chromatography-Quadrupole Time-Of-Flight Mass Spectrometry. Journal of exposure science & environmental epidemiology, 28(2), 101-108.
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3

Urinary Cotinine and NNAL Quantification

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A high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) system comprising a 1100 HPLC system (Agilent, Santa Clara, CA, USA) and API 4000 (AB Sciex, Redwood City, CA, USA) was used to measure the urinary cotinine levels. An HPLC-MS/MS system comprising a 1200 HPLC system (Agilent, Santa Clara, CA, USA) and a Triple Quadrupole 5500 (AB Sciex, Redwood City, CA, USA) was used to measure urinary NNAL concentrations. The limits of detection (LoD) of urinary cotinine and NNAL were 0.2740 µg/L and 0.1006 ng/L, respectively.
, & Lee H.S. (2022). Diagnostic Performance Evaluation of the Novel Index Combining Urinary Cotinine and 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol in Smoking Status Verification and Usefulness for Trend Monitoring of Tobacco Smoking Exposure. International Journal of Environmental Research and Public Health, 19(19), 12147.
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4

Anthropometric, Biomarker, and Toxicant Measurements

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Well-trained staff followed standard procedures to obtain anthropometric measurements. Trained staff measured the participants’ weight and height to the nearest 0.1 kg and 0.1 cm, respectively, while wearing light clothing but no shoes. Body mass index (BMI, kg/m2) was calculated as weight (kg) divided by squared height (m2). Blood pressure was obtained using a standard mercury sphygmomanometer with participants in a seated position and measured by a specialized investigator. Participants' blood samples were obtained from the antecubital vein in the morning after an overnight fast. Serum cholesterol and fasting plasma glucose (FPG) levels were measured enzymatically using a Hitachi Automatic Analyzer 7600-210 (Hitachi, Tokyo, Japan). Urine NNAL concentrations were measured by the HPLC-MS/MS method using an Agilent 1200 Series and Triple Quadrupole 5500 (AB Sciex, Framingham, MA, USA).
Lee J.W, & Kim S. (2023). Comparison of a Tobacco-Specific Carcinogen in Tobacco Cigarette, Electronic Cigarette, and Dual Users. Journal of Korean Medical Science, 38(19), e140.
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5

Vitamin D and Calcium Metabolites Measurement

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Serum 25-OH vitamin D2 + D3 was analyzed using high-performance liquid chromatography-tandem mass spectrometry on mass spectrometers API3000™ or API5500™ (Applied Biosystems, Lincoln, OR, USA). An amount of 1,25(OH)2 vitamin D3 was measured using liquid chromatography–mass spectrometry and detected by Electrospray Ionization Tandem Mass Spectrometry. The 5500 Triple Quadrupole or 6500 QTRAP (AB Sciex, Framingham, MA, USA) were used for these analyses. Serum ionized calcium was measured by a potentiometric method on a Nova CRT 8 electrolyte analyzer (Diamond Diagnostics Inc., Holliston, MA, USA). Plasma PTH was measured by an immunometric assay and plasma phosphate by absorption photometry, both on a Cobas® 6000 (Roche Diagnostics, Indianapolis, IN, USA).
Bendix M., Dige A., Jørgensen S.P., Dahlerup J.F., Bibby B.M., Deleuran B, & Agnholt J. (2020). Decrease in Mucosal IL17A, IFNγ and IL10 Expressions in Active Crohn’s Disease Patients Treated with High-Dose Vitamin D Alone or Combined with Infliximab. Nutrients, 12(12), 3699.
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6

Vitamin D and Mineral Metabolism Analysis

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Serum 25-OH vitamin D2 + D3 was analysed using high-performance liquid chromatography-tandem mass spectrometry on mass spectrometers API3000 TM or API5500 TM (Applied Biosystems, Lincoln, OR, USA). 1.25-dihydroxyvitamin D3 was measured using liquid chromatography mass spectrometry and detected by Electrospray Ionization Tandem Mass Spectrometry. The 5500 Triple Quadrupole or 6500 QTRAP (AB Sciex, Framingham, MA, USA) were used for these analyses. Serum ionised calcium was measured by a potentiometric method on a Nova CRT 8 electrolyte analyser (Diamond Diagnostics Inc., Holliston, MA, USA). Plasma PTH was measured by an immunometric assay and plasma phosphate by absorption photometry, both on a Cobas® 6000 (Roche Diagnostics, IN, USA).
Bendix M., Dige A., Jørgensen S.P., Dahlerup J.F., Bibby B.M., Deleuran B, & Agnholt J. (2021). Seven Weeks of High-Dose Vitamin D Treatment Reduces the Need for Infliximab Dose-Escalation and Decreases Inflammatory Markers in Crohn’s Disease during One-Year Follow-Up. Nutrients, 13(4), 1083.
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