Curcumin, copper(II) sulfate pentahydrate (CuSO4·5H2O), zinc(II) chloride (ZnCl2) and iron(III) nitrate nonahydrate (Fe(NO3)3·9H2O) were purchased from Merck (Germany). Solvents with highest purity were bought from Merck (Germany) and used without further purifications. The elemental analyses (carbon, hydrogen and nitrogen) of complexes were obtained from a Carlo ERBA Model EA 1108 analyzer. The metal content of the complexes was determined by atomic absorption analysis on a Varian Spectra AA-220 equipment. Fourier transform infrared (FT-IR) spectroscopy was performed using a FT-IR Spectrometer Bruker Tensor 27 as KBr disks. Fresh stock solution of metal-CUR complexes (Cu-CUR, Zn-CUR, Fe-CUR) and free-CUR were prepared in dimethyl sulfoxide (DMSO) at the concentration of 1 mg/ml. N-Acyl-homoserine lactone (C6-HSL) (Sigma-Aldrich) was used at 20 µM in C. violaceum CV026 biosensor bioassay.
N acyl homoserine lactone c6 hsl
Manufactured by Merck Group
Sourced in Switzerland
N-Acyl-homoserine lactone (C6-HSL) is a chemical compound used in laboratory research. It is a type of quorum sensing molecule that plays a role in bacterial cell-to-cell communication. The core function of C6-HSL is to facilitate the coordination of gene expression in bacterial populations.
Automatically generated - may contain errors
Lab products found in correlation
Curcumin, by Merck Group (1 mentions)
Copper 2 sulfate pentahydrate, by Merck Group (1 mentions)
Iron 3 nitrate nonahydrate, by Merck Group (1 mentions)
Zinc 2 chloride, by Merck Group (1 mentions)
Model ea 1108, by Carlo Erba (1 mentions)
Ciprofloxacin, by Merck Group (1 mentions)
Spectra aa220, by Agilent Technologies (1 mentions)
Copper ii sulfate pentahydrate cuso4 5h2o, by Merck Group (1 mentions)
3 protocols using n acyl homoserine lactone c6 hsl
1
Synthesis and Characterization of Metal-Curcumin Complexes
2
Synthesis and Characterization of Copper-Ciprofloxacin Complex
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Copper(II) sulfate pentahydrate (CuSO4·5H2O) and ciprofloxacin were purchased from Sigma-Aldrich (Buchs, Switzerland) and solvents were bought from Merck (Germany). The elemental analyses (carbon, hydrogen and nitrogen) of the complex were obtained from a Carlo ERBA Model EA 1108 analyzer. The content of copper in the complex was determined by atomic absorption analysis on a Varian Spectra AA-220 equipment. Fourier transform infrared (FT-IR) spectroscopy was performed using a FT-IR Spectrometer Bruker Tensor 27 as KBr disks. Fresh stock solutions of Cu-CIP and free-CIP were prepared in distilled water at the concentration of 1 mg/ml. N-Acyl-homoserine lactone (C6-HSL) (Sigma-Aldrich) was used at 20 µM.
3
Chromobacterium violaceum Bacterial Strains Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bacterial strains used in this study, Chromobacterium violaceum CV026 and Chromobacterium violaceum ATCC 31532 (McClean et al., 1997 (link)), were kindly provided by Professor Robert J.C. McLean, Texas State University, USA. N-Acyl-homoserine lactone (C6-HSL) was purchased from Sigma (Buchs, Switzerland), aliquoted and used at 15 μM in C. violaceum CV026 cultures to induce violacein production. The bacterium was cultivated aerobically and maintained in Luria Bertani (LB) medium at 30 °C; the turbidity was measured at 620 nm and adjusted to match a 0.5 Mc-Farland density standard, which produces approximately 1 × 108 cfu/mL according to the Clinical and Laboratory Standards Institute (CLSI) (Choo et al., 2006 (link); CLSI, 2009 ).
Bacterial strains were reactivated and plated onto LB agar, and then incubated during 18 to 24 h at 30 °C. Colonies were checked by macroscopic (morphology, color and consistency) and microscopic characteristics (Gram staining), and also phenotipically identified. BBL CRYSTAL Enteric/Nonfermenter (E/NF) Identification (ID) kit, from Becton & Dickinson, was used to identify nonfermenting Gram-negative bacteria (Holmes et al., 1994 (link)). Strains were stored at −70 °C with 80% glycerol and subcultured twice on Sabouraud agar (Difco) prior to testing anti-QS to confirm its purity and viability.
Bacterial strains were reactivated and plated onto LB agar, and then incubated during 18 to 24 h at 30 °C. Colonies were checked by macroscopic (morphology, color and consistency) and microscopic characteristics (Gram staining), and also phenotipically identified. BBL CRYSTAL Enteric/Nonfermenter (E/NF) Identification (ID) kit, from Becton & Dickinson, was used to identify nonfermenting Gram-negative bacteria (Holmes et al., 1994 (link)). Strains were stored at −70 °C with 80% glycerol and subcultured twice on Sabouraud agar (Difco) prior to testing anti-QS to confirm its purity and viability.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!