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X cite power meter model xr2100

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The X-Cite Power Meter Model XR2100 is a compact, handheld device designed to measure the optical power output of light sources. It features a wide measurement range and supports a variety of detector heads to accommodate different wavelength ranges and power levels.

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Lab products found in correlation

Sp5 2 confocal microscope, by Leica (3 mentions) Ms 222 anesthetic, by Merck Group (2 mentions) Sp5 2, by Leica (2 mentions) Heated stage, by Warner Instruments (2 mentions) Hyd detectors, by Warner Instruments (2 mentions) Doxycycline, by Merck Group (2 mentions) Sp8 confocal microscope, by Leica (2 mentions) Dmem lacking phenol red, by Thermo Fisher Scientific (2 mentions) Hc pl apo 40 1.10 w corr cs2, by Leica (1 mentions)

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X-cite (19 citations)

5 protocols using x cite power meter model xr2100

1

Confocal Imaging of Zebrafish Embryos

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All confocal images were collected in photon counting mode on a Leica SP5 II or Leica SP8 confocal microscope with HyD detectors and a heated stage (Warner Instruments LLC, USA). The laser power was calibrated before each imaging session using an X-Cite Power Meter Model XR2100 (Lumen Dynamics), which measures the power of laser light emitted from the objective onto the stage. All embryos were mounted in 0.5 % low-melt agarose dissolved in fish water supplemented with 0.4 mg/ml MS-222 anesthetic (Sigma, catalogue number E10521) and imaged around 36 hpf unless otherwise noted.
Lau S., Feitzinger A., Venkiteswaran G., Wang J., Lewellis S.W., Koplinski C.A., Peterson F.C., Volkman B.F., Meier-Schellersheim M, & Knaut H. (2020). A negative feedback loop maintains optimal chemokine concentrations for directional cell migration. Nature cell biology, 22(3), 266-273.
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2

Confocal Imaging of Zebrafish Embryos

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All confocal images were collected in photon counting mode on a Leica SP5 II or Leica SP8 confocal microscope with HyD detectors and a heated stage (Warner Instruments LLC, USA). The laser power was calibrated before each imaging session using an X-Cite Power Meter Model XR2100 (Lumen Dynamics), which measures the power of laser light emitted from the objective onto the stage. All embryos were mounted in 0.5 % low-melt agarose dissolved in fish water supplemented with 0.4 mg/ml MS-222 anesthetic (Sigma, catalogue number E10521) and imaged around 36 hpf unless otherwise noted.
Lau S., Feitzinger A., Venkiteswaran G., Wang J., Lewellis S.W., Koplinski C.A., Peterson F.C., Volkman B.F., Meier-Schellersheim M, & Knaut H. (2020). A negative feedback loop maintains optimal chemokine concentrations for directional cell migration. Nature cell biology, 22(3), 266-273.
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3

Zebrafish Fluorescent Imaging Protocol

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All embryos were mounted in 0.5 % low melt agar dissolved in fish water and supplemented with 0.4 mg/ml MS-222 anesthetic.
The Tcf/Lef-miniP:dGFP Wnt reporter line was imaged using the Leica SP5 II confocal microscope with hybrid detectors set to photon counting mode and a Leica 40× NA 0.8 water dipping objective (HCX APO L 40×/0.80 W U-V-I). The pinhole was set to 1 airy unit. The 488 nm laser was set to 300 μW and the 561 nm laser was set to 57 μW.
The dusp6:GFP, fgf10a:fgf10a-GFP, fgf10a:secGFP, and anos1a:anos1a-GFP transgenic lines were imaged using the Leica SP5 II confocal microscope with hybrid detectors set to photon counting mode and a 40× NA 1.1 water immersion objective (HC PL APO 40×/1.10 W CORR CS2). The pinhole was set to 2 airy units (150 μm). The 488 nm laser was set to 300 μW and the 561 nm laser to 57 μW.
The laser power was calibrated before each imaging session using an X-Cite Power Meter Model XR2100 (Lumen Dynamics), which measures the power of laser light emitted from the objective onto the stage.
Wang J., Yin Y., Lau S., Sankaran J., Rothenberg E., Wohland T., Meier-Schellersheim M, & Knaut H. (2018). Anosmin1 shuttles Fgf to facilitate its diffusion, increase its local concentration and induce sensory organs. Developmental cell, 46(6), 751-766.e12.
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4

Visualizing Cxcl12a-Cxcr4b Signaling in Zebrafish

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Wild-type or cxcl12a mutant embryos transgenic for cxcr4b:cxcr4b-Kate2-IRES-EGFP-CaaX were mounted together in a 35 mm dish. Similarly, cxcr4b:cxcr4b-Kate2-IRES-EGFP-CaaX transgenic embryos with or without hsp70:cxcl12a transgene were mounted together in a 35 mm dish. For embryos overexpressing Cxcl12a, embryos were heat shocked for 30 min at 39.5 degrees Celsius before imaging. For cultured cells, cells were induced with 1 μg/mL doxycycline (Sigma Aldrich, catalogue number D9891) for 24 hours before addition of purified Cxcl12a. Cells were grown on single or 6-well 35 × 10 mm plates to about 50–90 % confluency at time of imaging. One hour before imaging, the media was replaced with dilutions of Cxcl12a in DMEM lacking phenol red (Gibco, catalogue number 21063–029). Images were acquired using the Leica SP5 II confocal microscope or a Leica SP8 confocal microscope and a Leica 40x NA 0.8 water dipping objective (HCX APO L 40x/0.80 W U-V-I). Four frame accumulations and Z-stacks of 1.05 μm section thickness were collected. The 488 nm laser power was set at 300 μW and 561nm laser power set to 1mW using an X-Cite Power Meter Model XR2100 (Lumen Dynamics).
Lau S., Feitzinger A., Venkiteswaran G., Wang J., Lewellis S.W., Koplinski C.A., Peterson F.C., Volkman B.F., Meier-Schellersheim M, & Knaut H. (2020). A negative feedback loop maintains optimal chemokine concentrations for directional cell migration. Nature cell biology, 22(3), 266-273.
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5

Visualizing Cxcl12a-Cxcr4b Signaling in Zebrafish

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Wild-type or cxcl12a mutant embryos transgenic for cxcr4b:cxcr4b-Kate2-IRES-EGFP-CaaX were mounted together in a 35 mm dish. Similarly, cxcr4b:cxcr4b-Kate2-IRES-EGFP-CaaX transgenic embryos with or without hsp70:cxcl12a transgene were mounted together in a 35 mm dish. For embryos overexpressing Cxcl12a, embryos were heat shocked for 30 min at 39.5 degrees Celsius before imaging. For cultured cells, cells were induced with 1 μg/mL doxycycline (Sigma Aldrich, catalogue number D9891) for 24 hours before addition of purified Cxcl12a. Cells were grown on single or 6-well 35 × 10 mm plates to about 50–90 % confluency at time of imaging. One hour before imaging, the media was replaced with dilutions of Cxcl12a in DMEM lacking phenol red (Gibco, catalogue number 21063–029). Images were acquired using the Leica SP5 II confocal microscope or a Leica SP8 confocal microscope and a Leica 40x NA 0.8 water dipping objective (HCX APO L 40x/0.80 W U-V-I). Four frame accumulations and Z-stacks of 1.05 μm section thickness were collected. The 488 nm laser power was set at 300 μW and 561nm laser power set to 1mW using an X-Cite Power Meter Model XR2100 (Lumen Dynamics).
Lau S., Feitzinger A., Venkiteswaran G., Wang J., Lewellis S.W., Koplinski C.A., Peterson F.C., Volkman B.F., Meier-Schellersheim M, & Knaut H. (2020). A negative feedback loop maintains optimal chemokine concentrations for directional cell migration. Nature cell biology, 22(3), 266-273.
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