The maze was elevated 40 cm above the floor and consisted of two open arms without walls and two closed arms with 10-cm tall walls. Each arm was 30 × 8 cm, connected by an 8 × 8 cm center area. Mice were habituated in the testing room 1 hour before testing. Mice were placed in the center zone of the EPM facing one of the open arms and were allowed to explore freely for 10 minutes. Locomotion was video recorded and analyzed using Viewer III tracking system (Biobserve). Total distance traveled, time and distance spent in the closed and open arms were quantified. Data were analyzed with Student’s t-test.
Viewer 3 tracking system
Manufactured by Biobserve
Sourced in Germany
The Viewer III tracking system is a laboratory equipment designed for precision tracking and monitoring of animal movements and behaviors. It utilizes advanced optical sensors and software algorithms to accurately record and analyze the position and locomotion of research subjects within a controlled environment. The core function of the Viewer III is to provide researchers with reliable and detailed data for various experimental applications.
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Lab products found in correlation
3 protocols using viewer 3 tracking system
1
Elevated Plus-Maze Exploration Behavior
2
Mouse Elevated Plus Maze Exploration
3
Spatial Working Memory Evaluation in Y-Maze
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Spatial working memory in the Y-maze was measured under indirect red light conditions, using a modified protocol [46 (link)]. A gray plastic Y-maze was used to evaluate spontaneous alternations reflecting spatial working memory. The maze consisted of three arms that were spaced 120° angle from each other (dimensions of each arm 40 × 10 × 17 cm). Mice were individually placed in the distal end of one arm and allowed to freely explore the entire maze for 10 min. A completed arm entry was defined as the entering of the mouse with all four limbs. The sequence of arm entries was recorded and analyzed using the Viewer III tracking system (Biobserve, Bonn, Germany). Visiting all three different arms consecutively was termed a ‘correct’ triad, and visiting one arm twice in three consecutive entries was termed a ‘wrong’ triad. Correct alternation percentage was calculated using the following formula: %Alternation = (Number of Alternations/[Total number of arm entries − 2]) × 100. The Y-maze was thoroughly cleaned between each mouse using 70% ethanol to avoid olfactory cues.
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