Anti ccr7 af700

Manufactured by BD

Anti-CCR7-AF700 is a fluorescent-labeled antibody targeting the CCR7 receptor. It is designed for use in flow cytometry applications to identify and analyze CCR7-expressing cells.

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Lab products found in correlation

Anti cd3 bv605, by BioLegend (2 mentions) Anti pd 1 fitc, by BD (2 mentions) Anti cd95 pe, by BD (2 mentions) Anti cd19 pacific blue, by Thermo Fisher Scientific (2 mentions) Anti cd27 pc5, by Beckman Coulter (2 mentions) Anti cd45ra pe cy7, by BD (2 mentions) Anti cd14 pacific blue, by Thermo Fisher Scientific (2 mentions) Anti cd4 v500, by BD (2 mentions) Anti cd8 apc h7, by BD (2 mentions) Anti cd45ro apc, by BD (2 mentions) Anti pe and anti apc microbeads, by Miltenyi Biotec (1 mentions) Anti cd14 apc h7, by BD (1 mentions) Anti cd19 apc h7, by BD (1 mentions) Anti cd38 bv786, by BD (1 mentions) Anti cd71 bv421, by BD (1 mentions) Anti cd27 bv711, by BD (1 mentions) Anti hla dr bv605, by BioLegend (1 mentions) Anti cd4 bv650, by BD (1 mentions) Anti pd 1 pe cy7, by BD (1 mentions) Anti cd45ra fitc, by BD (1 mentions)

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Anti-CCR7 (10 citations)

3 protocols using anti ccr7 af700

Tetramer-Based Enrichment of Antigen-Specific T Cells

Cited 1 time

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PBMCs (2.4–10 × 10⁶) were stained with WT HLA-A2^S269–277-PE and variant HLA-A2^S269–277-APC tetramers at room temperature for 1 h in MACS buffer (PBS with 0.5% BSA and 2 mM EDTA). Cells were then incubated with anti-PE and anti-APC microbeads (Miltenyi Biotec), and tetramer⁺ cells were enriched using magnetic separation (9 (link)). Lymphocytes were stained with anti-CD71-BV421 (#562995), anti-CD4-BV650 (#563875), anti-CD27-BV711 (#563167), anti-CD38-BV786 (#563964), anti-CCR7-AF700 (#561143), anti-CD14-APC-H7 (#560180), anti-CD19-APC-H7 (#560177), anti-CD45RA-FITC (#555488), anti-CD8-PerCP-Cy5.5 (#565310), anti-CD95-PE-CF594 (#562395), anti-PD1-PE-Cy7 (#561272) (BD Biosciences), anti-CD3-BV510 (#317332), anti-HLA-DR-BV605 (#307640) (BioLegend), and LIVE/DEAD near-infrared stain (#L10119, Invitrogen) for 30 min, fixed with 1% PFA before acquiring data on an LSRII Fortessa (BD Bioscience). FCS files were analyzed using FlowJo v10 software.

Chaurasia P., Nguyen T.H., Rowntree L.C., Juno J.A., Wheatley A.K., Kent S.J., Kedzierska K., Rossjohn J, & Petersen J. (2021). Structural basis of biased T cell receptor recognition of an immunodominant HLA-A2 epitope of the SARS-CoV-2 spike protein. The Journal of Biological Chemistry, 297(3), 101065.

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Multiparametric Flow Cytometry for Immune Profiling

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The following fluorochrome-conjugated monoclonal antibodies (mAbs) were purchased from commercial vendors and used for surface staining: anti-CD4-V500, anti-CD8-APC-H7, anti-CD45RA-PE-Cy7, anti-CD45RO-APC, anti-CCR7-AF700, anti-CD95-PE, anti-CD160-FITC, anti-CD244-FITC, and anti-PD-1-FITC (BD Biosciences, San Jose, CA); anti-CD3-BV605 (Biolegend, San Diego, CA); anti-CD14-Pacific Blue and anti-CD19-Pacific Blue (Life Technologies, Carlsbad, CA); and anti-CD27-PC5 (Beckman Coulter, Indianapolis, IN). The fixable violet amine reactive dye (ViViD; Invitrogen/Molecular Probes, Eugene, OR) was used to eliminate dead cells by flow cytometry. For intracellular cytokine staining, the following mAbs were used: anti-granzyme B (GZMB)-FITC, anti-IL-2-FITC, and anti-IFN-γ-FITC (BD Biosciences).

Hosokawa K., Muranski P., Feng X., Townsley D.M., Liu B., Knickelbein J., Keyvanfar K., Dumitriu B., Ito S., Kajigaya S., Taylor JG V.I., Kaplan M.J., Nussenblatt R.B., Barrett A.J., O’Shea J, & Young N.S. (2016). Memory Stem T Cells in Autoimmune Disease: High Frequency of Circulating CD8+ Memory Stem Cells in Acquired Aplastic Anemia. Journal of immunology (Baltimore, Md. : 1950), 196(4), 1568-1578.

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Multiparametric Flow Cytometry Analysis

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The following fluorochrome-conjugated monoclonal antibodies (mAbs) were purchased from commercial vendors and used for surface staining: anti-CD4-V500, anti-CD8-APC-H7, anti-CD45RA-PE-Cy7, anti-CD45RO-APC, anti-CCR7-AF700, anti-CD95-PE, anti-PD-1-FITC and anti-CD69-FITC (BD Biosciences, San Jose, CA); anti-CD3-BV605 (Biolegend, San Diego, CA); anti-CD14-Pacific Blue and anti-CD19-Pacific Blue (Life Technologies, Carlsbad, CA); anti-CD30 Ligand /TNFSF8-AF488 and anti- A20/TNFAIP3- AF488 (Novus Biologicals, Littleton, CO) and anti-CD27-PC5 (Beckman Coulter, Indianapolis, IN). The fixable violet amine reactive dye (ViViD; Invitrogen/Molecular Probes, Eugene, OR) was used to eliminate dead cells by flow cytometry.

Hosokawa K., Kajigaya S., Keyvanfar K., Qiao W., Xie Y., Townsley D.M., Feng X, & Young N.S. (2017). T Cell Transcriptomes from Paroxysmal Nocturnal Hemoglobinuria Patients Reveal Novel Signaling Pathways. Journal of immunology (Baltimore, Md. : 1950), 199(2), 477-488.

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