8-week-old mice received an intraperitoneal injection of a mixture of ketamine/xylazine (87.5 mg/kg Ketamine (VetaKet®, AKORN, Lake Forest, IL)/12.5 mg/kg Xylazine (Anased, Lloyd Laboratories®, Shenandoah, IA). Upon anesthesia, eyes were hydrated with balanced salt solution (BSS; Alcon Laboratories, Fort Worth, TX). To obtain retinal images, the tear film was wicked away and eyes were imaged with a Bioptigen spectral domain optical coherence tomographer (SD-OCT; Bioptigen, USA) using the mouse retinal bore. The volume intensity projection was centered on the optic nerve. Following imaging, eyes were hydrated with lubricant ophthalmic ointment (Artificial Tears, AKORN, Lake Forest, IL) and mice were provided supplemental indirect warmth for anesthesia recovery. Retinal ganglion cell complex (RGCC; inner limiting membrane to the innermost border of the inner nuclear layer) thickness was measured from retinal images within the Bioptigen InVivoVue Clinic software four times per eye using vertical angle-locked B-scan calipers.
Vetaket
Manufactured by Akorn
Sourced in United States
VetaKet is a laboratory equipment product. It is designed to perform specific functions in a laboratory setting.
Automatically generated - may contain errors
4 protocols using vetaket
1
Quantifying Retinal Ganglion Cell Complex in Mouse
2
Ketamine Anesthesia and Heparin Administration in Rats
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All rats were intraperitoneally injected with 30 mg kg−1 ketamine hydrochloride (VetaKet, Akorn, Inc., Lake Forest, IL, USA), and anesthesia was evaluated after 10 min with the toe pinch method. With no reaction to the toe pinch, the animals were intraperitoneally injected with 1000 units kg−1 heparin (West-Ward Pharmaceuticals, Eatontown, NJ, USA). The animals were euthanized by decapitation 5 min after heparin administration.
3
Anesthesia Protocol for Percutaneous Disc Injections
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A standard anesthesia protocol was used for percutaneous intradiscal injections and MRI. Sheep were premedicated with xylazine (AnaSed; Akorn, Lake Forest, IL, USA; 0.03 mg/kg IV) and butorphanol (Torbugesic; Zoetis, Kalamazoo, MI USA, 0.01 mg/kg IV) and induced with a combination of ketamine (VetaKet; Akorn, 2.2 mg/kg IV) and diazepam (Hospira, Lake Forest, IL USA, 0.06 mg/kg IV). After local application of a few drops of a 2% lidocaine (AnaSed; Akorn) solution on the vocal folds, sheep were intubated and anesthesia was maintained using isoflurane. Throughout anesthesia, lactated Ringer’s solution was administered (10 mL/kg/hr). Post-operatively, buprenorphine (Par Pharmaceutical, Chestnut Ridge, NY USA, 0.005 mg/k IV) and flunixin meglumine (Merck & Co., Kenilworth, NJ, USA, 1.1 mg/kg IM) were administered for analgesia.
4
Multimodal Cortical Neurophysiology
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Each animal was anesthetized with an intramuscular injection of a mixture of ketamine (40 mg/kg; VetaKet, NDC 59399-114-10, Akorn, Lake Forest, IL) and xylazine (10 mg/kg; XylaMed, ANADA 200-529, Bimeda, Le Sueur, MN) with supplements every 45-60 min to maintain an areflexive state. Heart rate and blood oxygenation was continuously monitored using a pulse oximeter (H100, Edan Instruments, Shenzhen, China), and body temperature was maintained at 38.0 ± 0.5 C using a heating blanket and rectal thermometer. Each animal was fixed in a stereotaxic frame (1430, David Kopf Instruments, Tujunga, CA), and a craniotomy was performed to expose the right auditory, visual, and somatosensory cortices.
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