The B-cell neoplastic cell lines MEC1 and JVM2 were acquired from DSMZ (Braunschweig, Germany) and ATCC (Manassas, VA), respectively. GM12865 and GM12891 are lymphoblastoïd cell lines from normal B-cells of the NIGMS Human Genetic Cell Repository. They were obtained from the Coriell Institute for medical Research (Camden, NJ, USA). Cell lines were cultured in RPMI-1640 supplemented with 10% of FBS, 1% sodium-pyruvate, 1% L-glutamine and 1% penicillin/streptomycin at 37 °C and 5% CO2. All culture reagents were from Wisent, St-Bruno, QC, Canada. Cell models were not passaged for more than 2 months and were regularly checked for mycoplasms.
Jvm 2
Manufactured by Leibniz Institute DSMZ
Sourced in Germany
The JVM-2 is a laboratory instrument designed for precise mass analysis. It utilizes advanced ion trap technology to perform accurate measurements of molecular masses. The core function of the JVM-2 is to provide high-resolution mass spectrometry capabilities for scientific research and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin edta, by Thermo Fisher Scientific (3 mentions)
Granta 519, by Leibniz Institute DSMZ (2 mentions)
Jeko 1, by Leibniz Institute DSMZ (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Mec 1, by Leibniz Institute DSMZ (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Rec 1, by Leibniz Institute DSMZ (1 mentions)
Diclofenac, by MP Biomedicals (1 mentions)
Lipofectamine ltx, by Thermo Fisher Scientific (1 mentions)
Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions)
Gentamicin, by Thermo Fisher Scientific (1 mentions)
Glutamax supplement, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Jurkat, by Leibniz Institute DSMZ (1 mentions)
Namalwa, by Leibniz Institute DSMZ (1 mentions)
5 protocols using jvm 2
1
Culturing Neoplastic and Normal B-cell Lines
2
Evaluating CDKN2A Deletion in FFPE Mantle Cell Lymphoma
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To obtain insight into the reliability of testing numerical genetic alterations (especially deletions) in FFPE tissues, mantle cell lymphoma cell lines REC1 and JVM2 (DSMZ, Braunschweig, Germany) were utilized. These cell lines are well-characterized on the genetic level [19] (link). With special regard to the 9p21 locus, REC1 was shown to harbor a homozygous deletion of CDKN2A, while JVM2 retains two copies of CDKN2A[19] (link). Cell lines were cultured in RPMI-1640 medium (Life Technologies, Darmstadt, Germany), supplemented with 10% heat inactivated fetal bovine serum, 50 µg/ml streptomycin and penicillin (Life Technologies) and maintained in a humidified 95% air – 5% CO2 atmosphere at 37°C. In an experimental setting, REC1 cells were serially spiked into JVM2 cell suspensions to obtain suspensions with a defined content of CDKN2A-deleted cells (0%, 25%, 50%, 75%, 100% cells with deletion of CDKN2A). For comparison with the routine setting, 4×106 cells of the cellular suspensions were washed with 100% isopropanol, fixed with buffered formalin and embedded into paraffin. Subsequent FISH analyses were performed on whole tissue sections.
3
MCL Cell Line Protocol and Therapeutic Evaluation
4
Cell Line Maintenance and MCL/CLL Sample Collection
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The MCL cell lines JeKo-1, Granta519, JVM-2 and the T-cell line Jurkat were obtained from DSMZ (Braunschweig, Germany). All cell lines were maintained in culture in RPMI 1640 medium, GlutaMAX supplement, and HEPES (RPMI-GM, Gibco, UK), supplemented with 50 µg/mL of gentamicin and 10% fetal bovine serum (FBS) (Gibco) under the conditions of 5% CO2 at 37 °C. The glioblastoma cell line U-251 was purchased from ECACC and was kept in culture in MEM complete medium supplemented with 10% FBS (Sigma-Aldrich/Merck, Darmstadt, Germany) at 5% CO2 at 37 °C, until confluency before splitting them using trypsin-EDTA (0.25%, ThermoFisher Scientific, Paisley, UK) to detach the cells. MCL and CLL blood samples were collected at Karolinska University Hospital, Huddinge. The study was approved by the Regional Central Ethical Review Board at Karolinska Institutet and patients gave informed consent, in compliance with the Declaration of Helsinki.
5
Relapsed Multiple Myeloma Therapy Comparison
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We conducted a prospective randomized phase 3 trial in MM at first relapse comparing the activity of bortezomib, cyclophosphamide, and dexamethasone versus lenalidomide, cyclophosphamide, and dexamethasone as secondline therapy. Within this study, 25 patients who achieved complete response during therapy (International Myeloma Working Group guidelines) 9 and had longitudinal biological samples collected for disease monitoring were analyzed. Our institutional review board approved this study (INT 57/ 10), and patients provided informed consent.
BM and PB samples were obtained during routine clinical evaluations at study entry; after 3, 6, and 9 cycles of therapy; and at follow-up time points. Plasma was obtained processing PB samples collected in K2-EDTA tubes (BD Vacutainer, Becton Dickinson, Franklin Lakes, NJ) within 3 hours, with a first centrifugation at 1500 Â g for 10 minutes and a second high-speed centrifugation at 16,000 Â g for 10 minutes at 4 C. Plasma samples were stored at À80 C until extraction. 10 PB of 10 healthy donors was also collected. Namalwa (human Burkitt lymphoma; ACC 24) and JVM-2 (human chronic B-cell leukemia; ACC 12) cell lines were from DSMZ (Braunschweig, Germany).
BM and PB samples were obtained during routine clinical evaluations at study entry; after 3, 6, and 9 cycles of therapy; and at follow-up time points. Plasma was obtained processing PB samples collected in K2-EDTA tubes (BD Vacutainer, Becton Dickinson, Franklin Lakes, NJ) within 3 hours, with a first centrifugation at 1500 Â g for 10 minutes and a second high-speed centrifugation at 16,000 Â g for 10 minutes at 4 C. Plasma samples were stored at À80 C until extraction. 10 PB of 10 healthy donors was also collected. Namalwa (human Burkitt lymphoma; ACC 24) and JVM-2 (human chronic B-cell leukemia; ACC 12) cell lines were from DSMZ (Braunschweig, Germany).
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