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Rpmi 1640 modified media

Manufactured by Merck Group
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RPMI-1640 modified media is a commonly used cell culture medium. It provides a balanced salt solution and nutrients to support the growth and maintenance of a variety of cell types in vitro.

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Lab products found in correlation

Mitosox red, by Thermo Fisher Scientific (2 mentions) Acetaminophen, by Merck Group (2 mentions) Collagenase a from clostridium histolyticum, by Roche (2 mentions) Glutathione (gsh), by Merck Group (2 mentions) Trypan blue solution, by Merck Group (2 mentions) 4 amino 5 methylamino 2 7 difluorofluorescein daf fm diacetate, by Thermo Fisher Scientific (2 mentions) Lactate dehydrogenase ldh cytotoxicity detection kit, by Roche (2 mentions) Atp bioluminescent assay kit, by Merck Group (2 mentions) S nitrosoglutathione, by Merck Group (2 mentions) Penicillin g, by Merck Group (2 mentions)

2 protocols using rpmi 1640 modified media

1

Oxidative Stress and Mitochondrial Dysfunction

Check if the same lab product or an alternative is used in the 5 most similar protocols
Acetaminophen (APAP; 4-acetamidophenol), Percoll, Hepes, (heparin sodium salt grade I-A from porcine intestinal mucosa), penicillin G (sodium salt), RPMI-1640 modified media (with L-glutamine and without sodium bicarbonate and phenol red), 0.4% trypan blue solution, GSH, GSSG, S-nitrosoglutathione (GSNO), 3NT, NADH, NAD, and trifluoperazine (TFP; 10-[3-(4-methylpiperazin-1-yl)propyl]-2-(trifluoromethyl)-10H-phenothiazine), ATP Bioluminescent Assay kit, were products of Sigma Chemical Company (St Louis, MO). Collagenase A from Clostridium histolyticum was purchased from Roche Diagnostics (Indianapolis, IN). MitoSOX Red, 4-Amino-5-methylamino-2′,7′-difluorofluorescein diacetate (DAF-FM), and JC1 were obtained from Life technologies (Eugene, OR). LDH (lactate dehydrogenase) cytotoxicity detection kit was a product of from Roche Diagnostic Corporation (Indianapolis).
Banerjee S., Melnyk S.B., Krager K.J., Aykin-Burns N., McCullough S.S., James L.P, & Hinson J.A. (2017). Trifluoperazine inhibits acetaminophen-induced hepatotoxicity and hepatic reactive nitrogen formation in mice and in freshly isolated hepatocytes. Toxicology Reports, 4, 134-142.
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2

Oxidative Stress and Mitochondrial Dysfunction

Check if the same lab product or an alternative is used in the 5 most similar protocols
Acetaminophen (APAP; 4-acetamidophenol), Percoll, Hepes, (heparin sodium salt grade I-A from porcine intestinal mucosa), penicillin G (sodium salt), RPMI-1640 modified media (with L-glutamine and without sodium bicarbonate and phenol red), 0.4% trypan blue solution, GSH, GSSG, S-nitrosoglutathione (GSNO), 3NT, NADH, NAD, and trifluoperazine (TFP; 10-[3-(4-methylpiperazin-1-yl)propyl]-2-(trifluoromethyl)-10H-phenothiazine), ATP Bioluminescent Assay kit, were products of Sigma Chemical Company (St Louis, MO). Collagenase A from Clostridium histolyticum was purchased from Roche Diagnostics (Indianapolis, IN). MitoSOX Red, 4-Amino-5-methylamino-2′,7′-difluorofluorescein diacetate (DAF-FM), and JC1 were obtained from Life technologies (Eugene, OR). LDH (lactate dehydrogenase) cytotoxicity detection kit was a product of from Roche Diagnostic Corporation (Indianapolis).
Banerjee S., Melnyk S.B., Krager K.J., Aykin-Burns N., McCullough S.S., James L.P, & Hinson J.A. (2017). Trifluoperazine inhibits acetaminophen-induced hepatotoxicity and hepatic reactive nitrogen formation in mice and in freshly isolated hepatocytes. Toxicology Reports, 4, 134-142.
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