Human tgf β1 duo set

To measure IgA levels in colon tissues, feces and mucus were flushed and homogenized with endotoxin-free PBS. The supernatants were harvested after centrifugation (800G, 15 min) and stored at −80°C for later analysis. The cell supernatants of sort-purified B cells that underwent in vitro culture were also collected for cytokine and antibody measurements. The concentrations of IgA, IL-10, IL-6, and IgM were measured using the Mouse Ready-SET-Go! ELISA kits (eBioscience) according to the manufacturer’s instructions. The levels of active TGF-β were measured using a human TGF-β1 duo-set (DY240) or mouse TGF-β1 duo-set (DY1679) with a substrate reagent pack (DY999) according to the manufacturer’s instructions (R&D Systems Europe, Abingdon, UK) (25 (link), 26 (link)).

Four PRP hyaluronan collagen composite matrix constructs of each of the 4 volunteers were cultured in vitro over a period of 8 days in 1 mL autologous plasma.
Concentrations of the growth factors PDGF, TGFβ1, and VEGF were measured by ELISA technique at 0 h, 8 h, 12 h, 24 h, 48 h, and 192 h (8 days) using kits from R&D Systems: Human PDGF-AB DuoSet (DY222), Human TGFβ1 DuoSet (DY240), and Human VEGF DuoSet (DY293B). Results of cultured empty control scaffolds were subtracted from the growth factor concentrations obtained from the cultured PRP loaded scaffolds in order to exclude an influence of the remaining small growth factor activity in the autologous plasma.